Product Info Summary
SKU: | M03824 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-AFF4 Picoband™ Antibody (monoclonal, 8G12)
SKU/Catalog Number
M03824
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AFF4 Picoband™ Antibody (monoclonal, 8G12) catalog # M03824. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AFF4 Picoband™ Antibody (monoclonal, 8G12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03824)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
8G12
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human AFF4, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03824 is reactive to AFF4 in Human
Applications
M03824 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
150 kDa
Calculated molecular weight
127.459kDa
Background of AFF4
The AFF4 gene encodes a scaffold protein that functions as a core component of the super elongation complex (SEC), which is involved in transcriptional regulation during embryogenesis. The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. This gene is mapped to chromosome 5q31.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AFF4 using anti-AFF4 antibody (M03824).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human HEPG2 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human HEK293 whole cell lysates,
Lane 5: human MDA-MB-453 whole cell lysates,
Lane 6: human PANC-1 whole cell lysates,
Lane 7: human SW620 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AFF4 antigen affinity purified monoclonal antibody (Catalog # M03824) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for AFF4 at approximately 150KD. The expected band size for AFF4 is at 150KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of 293T cells using anti-AFF4 antibody (M03824).
Overlay histogram showing 293T cells stained with M03824 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AFF4 Antibody (M03824, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For AFF4 (Source: Uniprot.org, NCBI)
Gene Name
AFF4
Full Name
AF4/FMR2 family member 4
Weight
127.459kDa
Superfamily
AF4 family
Alternative Names
AF4/FMR2 family, member 4; AF5Q31AF4/FMR2 family member 4; ALL1-fused gene from chromosome 5q31 protein; Major CDK9 elongation factor-associated protein; MCEFALL1 fused gene from 5q31; MGC75036; Protein AF-5q31 AFF4 AF5Q31, CHOPS, MCEF AF4/FMR2 family member 4 AF4/FMR2 family member 4|ALL1-fused gene from chromosome 5q31 protein|major CDK9 elongation factor-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AFF4, check out the AFF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AFF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AFF4 Picoband™ Antibody (monoclonal, 8G12) (M03824)
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