Rabbit IgG polyclonal antibody for Nuclease-sensitive element-binding protein 1(YBX1) detection. Tested with WB in Human;Mouse;Rat.
Anti-YB1/YBX1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-YB1/YBX1 Antibody
See all YB1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for YB 1/YBX1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. YB 1/YBX1 information: Molecular Weight: 35924 MW; Subcellular Localization: Cytoplasm. Nucleus. Cytoplasmic granule . Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.|
|Cite This Product||Anti-YB1/YBX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1758)|
|Similar Products From
|Anti-YB1/YBX1 Antibody may replace the following items: sc 86712.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human YB1(137-152aa KYAADRNHYRRYPRRR), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For YBX1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||4904|
|Species Of This Entry||Human|
|Protein Name||Y-box-binding protein 1|
|Alternative Names||YB1|BP-8; CBF-A; class II, Y box-binding protein I; CSDA2; CSDB; DBPB CCAAT-binding transcription factor I subunit A; EFI-A; Enhancer factor I subunit A; MDR-NF1; MGC104858; MGC110976; MGC117250; NSEP1 Y-box-binding protein 1; nuclease-sensitive element-binding protein 1; Y box binding protein 1; YB1 DNA-binding protein B; YB-1 Y-box transcription factor; YBX1|
|Post Tranlational Modifications||Phosphorylation; Methylation; Cleavage; Acetylation; Reduction; Glycosylation; Oxidation; Demethylation; Farnesylation; Dephosphorylation; Ubiquitination|
|Subcellular Localization||Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Secreted, extracellular exosome. Predominantly cytoplasmic in proliferating cells (PubMed:12604611). Cytotoxic stress and DNA damage enhance translocation to the nucleus (PubMed:14718551). Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:25229427). Shuttles between nucleus and cytoplasm (PubMed:25229427). Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress (PubMed:18335541). Secreted by mesangial and monocytic cells after inflammatory challenges (PubMed:19483673).|
|Gene Location||1p34.2, on Chromasome 1, gene sequence: NC_000001.11 (42682418..42703805)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For YBX1 (Source: Uniprot.org, NCBI)
|Protein Function||Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro.|
|Interacting Proteins||C1QBP; DHX9; EP300; FBXO33; LSM3; PITX2; PPM1G; TPT1|
|Research Areas||Breast Cancer; Cancer; DNA Repair; Epigenetics; Ovarian Carcinoma Cell Markers; Stem Cell Markers; Transcription Factors and Regulators; Translation Control|
|Related Diseases||Neoplasms; Malignant Neoplasms; Mammary Neoplasms; Neurofibromatosis 1; Malignant Neoplasm Of Breast; Carcinoma; Malignant Paraganglionic Neoplasm; Cell Transformation, Neoplastic; Liver Carcinoma; Neoplasm Metastasis; Cell Invasion; Leukemia; Genotoxic Stress; Glioma; Colonic Neoplasms; Sarcoma; Adenocarcinoma; Leukoencephalopathies; Malignant Neoplasm Of Lung; Nervousness|
|Related Pathways||Dna Replication; Translation; Localization; Drug Resistance; Cell Proliferation; Methylation; Dna Repair; Cell Cycle; Cell Growth; Pathogenesis; Regulation Of Gene Expression; Transport; Rna Interference; Viral Replication; Chromatin Remodeling; Gene Silencing; Rna Splicing; Single-stranded Dna Binding; Rna Processing; Lactation|
|Background||YBX1(Y box binding protein 1), commonly referred to as "YB-1" by researchers, is a human protein. YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414 and contains 18% basic residues and putative nuclear localization signals. The YBX1 gene is mapped on 1p34.2. Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1(EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation.|
|Scientific References||PMID: 2977358 by Sakura H., et al. Two human genes isolated by a novel method encode DNA-binding proteins containing a common region of homology.|
PMID: 3174636 by Didier D.K., et al. Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box.
Our Boster Quality Guarantee for Anti-YB1/YBX1 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti YBX1 (PA1758) at 0.5ug/ml
WB: HT1080Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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