Product Info Summary
| SKU: | M00026-4 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: OTI1A6]
SKU/Catalog Number
M00026-4
Size
100 µl
Description
Boster Bio c-Myc (c-Myc ) mouse monoclonal antibody, clone OTI1A6 (formerly 1A6). Catalog# M00026-4. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: OTI1A6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00026-4)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1A6
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human Myc (NP_002458) produced in E.coli.
Reactive Species
M00026-4 is reactive to MYC in Human, Mouse, Rat
Calculated molecular weight
48.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00026-4 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:50
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYC.
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Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-MYC monoclonal antibody at 1:200 dilution.
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-MYC monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either MYC (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYC antibody (M00026-4)
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Anti-MYC mouse monoclonal antibody (M00026-4) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MYC.
Specific Publications For Anti-c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: OTI1A6] (M00026-4)
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