SKU MA1034
Size 100μg/vial
Reactivity Human, Mouse
Clonality Monoclonal
Clone Number DCS-3
Host Mouse
Ig Isotype Mouse IgG2a
Applications ICC, WB

Overview

Product Name Anti-Cyclin D2 Antibody (Monoclonal, DCS-3)
SKU/Catalog Number MA1034
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Cyclin D2, cyclin D2 (CCND2) detection. Tested with WB, ICC in Human;mouse. No cross reactivity with other proteins.
Cite This Product Anti-Cyclin D2 Antibody (Monoclonal, DCS-3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1034)
Host Mouse
Immunogen Recombinant human cyclin D2 protein.
Reactivity Human, Mouse

Assay Details

Assay Dilutions Overview

Immunocytochemistry , 1μg/ml, Human, mouse, -
Western blot, 2-4μg/ml, Human, mouse

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q04827
Gene Name Ccnd2
Protein Name G1/S-specific cyclin-D2
Alternative Names G1/S-specific cyclin-D2;Vin-1 proto-oncogene;Ccnd2;Vin-1;
Subcellular Localization Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. .
Molecular Weight 32826 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity). .
Background Inaba et al.(1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2(CCND2) gene ia assigned to 12p13.

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Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240
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Publications

Sun L, Carr Al, Li P, Lee J, Mcgregor M, Li L. Biochem Biophys Res Commun. 2014 Jul 11;449(4):444-8. Doi: 10.1016/J.Bbrc.2014.05.048. Epub 2014 May 20. Characterization Of The Human Oncogene Scl/Tal1 Interrupting Locus (Stil) Mediated Sonic Hedgeh...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.