|Ig Isotype||Mouse IgG2a|
|Product Name||Anti-Cyclin D2 Antibody (Monoclonal, DCS-3)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Cyclin D2, cyclin D2 (CCND2) detection. Tested with WB, ICC in Human;mouse. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cyclin D2 Antibody (Monoclonal, DCS-3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1034)|
|Immunogen||Recombinant human cyclin D2 protein.|
Assay Dilutions Overview
Western blot, 2-4μg/ml, Human, mouse
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
Protein Target Info (Source: Uniprot.org)
|Protein Name||G1/S-specific cyclin-D2|
|Alternative Names||G1/S-specific cyclin-D2;Vin-1 proto-oncogene;Ccnd2;Vin-1;|
|Subcellular Localization||Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. .|
|Molecular Weight||32826 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity). .|
|Background||Inaba et al.(1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2(CCND2) gene ia assigned to 12p13.|
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