Anti-ZEB1 Monoclonal Antibody

ZEB1 antibody

Boster Bio Anti-ZEB1 Monoclonal Antibody catalog # M00548. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M00548
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-ZEB1 Monoclonal Antibody

View all ZEB1 Antibodies

SKU/Catalog Number

M00548

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ZEB1 Monoclonal Antibody catalog # M00548. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ZEB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00548)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADIO-26

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human AREB6

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00548 is reactive to ZEB1 in Human

Applications

M00548 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa, 48 kDa

Calculated molecular weight

124.074kDa

Background of ZEB1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ZEB1 (Source: Uniprot.org, NCBI)

Gene Name

ZEB1

Full Name

Zinc finger E-box-binding homeobox 1

Weight

124.074kDa

Superfamily

delta-EF1/ZFH-1 C2H2-type zinc-finger family

Alternative Names

AREB6; AREB6MGC133261; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; Negative regulator of IL2; NIL-2-A zinc finger protein; NIL2A; NIL-2-A; posterior polymorphous corneal dystrophy 3; PPCD3; TCF8; TCF-8; TCF8BZP; transcription factor 8 (represses interleukin 2 expression); Transcription factor 8; ZEB; ZEB1; ZFHEP; ZFHX1A; zinc finger E-box binding homeobox 1; zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein ZEB1 AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A zinc finger E-box binding homeobox 1 zinc finger E-box-binding homeobox 1|delta-crystallin enhancer binding factor 1|negative regulator of IL2|posterior polymorphous corneal dystrophy 3|transcription factor 8 (represses interleukin 2 expression)|zinc finger homeodomain enhancer-binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ZEB1, check out the ZEB1 Infographic

ZEB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ZEB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00548 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang Q,Shang J,Zhang Y,Zhou Y,Tang L.MiR-451a restrains the growth and metastatic phenotypes of papillary thyroid carcinoma cells via inhibiting ZEB1.Biomed Pharmacother.2020 Jul;127:109901.doi:10.1016/j. biopha.2020.109901.Epub 2020 Apr 23.PMID:32335297.
Species: Human,Mouse
M00548 usage in article: APP:IF, SAMPLE:SW579 CELL AND B-CPAP CELL, DILUTION:1:500

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4 Customer Q&As for Anti-ZEB1 Monoclonal Antibody

Question

We have seen staining in human leukemic t-cell. Are there any suggestions? Is anti-ZEB1 Monoclonal antibody supposed to stain leukemic t-cell positively?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

From literature leukemic t-cell does express ZEB1. From Uniprot.org, ZEB1 is expressed in myometrium, brain thalamus, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have ZEB1 expression, here are a few articles citing expression in various tissues:
Brain, and Thalamus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2020-04-22

Question

We bought anti-ZEB1 Monoclonal antibody for WB on cervix carcinoma erythroleukemia in the past. I am using human, and We are going to use the antibody for IF next. I am interested in examining cervix carcinoma erythroleukemia as well as myometrium in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

I have checked the website and datasheets of our anti-ZEB1 Monoclonal antibody and I see that M00548 has been validated on human in both WB and IF. Thus M00548 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-11

Question

We are currently using anti-ZEB1 Monoclonal antibody M00548 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

The anti-ZEB1 Monoclonal antibody (M00548) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-11

Question

Our lab were content with the WB result of your anti-ZEB1 Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is ZEB1 supposed to be expressed?

S. Banerjee

Verified customer

Asked: 2013-02-28

Answer

Based on literature, cervix carcinoma erythroleukemia does express ZEB1. Generally ZEB1 expresses in nucleus. Regarding which tissues have ZEB1 expression, here are a few articles citing expression in various tissues:
Brain, and Thalamus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2013-02-28

Order DetailsPrice
M00548

100μL

$370
M00548-30ul

30μL sample

$99
M00548-carrier-free

Carrier Free

$370

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