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Product Info Summary
| SKU: | M00548 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ZEB1 Monoclonal Antibody
SKU/Catalog Number
M00548
BM5462 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ZEB1 Monoclonal Antibody catalog # M00548. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZEB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00548)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADIO-26
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AREB6
Reactive Species
M00548 is reactive to ZEB1 in Human
Observed Molecular Weight
200 kDa
Calculated molecular weight
124.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00548 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: HEK293 cell
Validation Images & Assay Conditions
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of AREB6 expression in HEK293 cell lysate.
Specific Publications For Anti-ZEB1 Monoclonal Antibody (M00548)
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4 Customer Q&As for Anti-ZEB1 Monoclonal Antibody
Question
We have seen staining in human leukemic t-cell. Are there any suggestions? Is anti-ZEB1 Monoclonal antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
From literature leukemic t-cell does express ZEB1. From Uniprot.org, ZEB1 is expressed in myometrium, brain thalamus, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have ZEB1 expression, here are a few articles citing expression in various tissues:
Brain, and Thalamus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2020-04-22
Question
We bought anti-ZEB1 Monoclonal antibody for WB on cervix carcinoma erythroleukemia in the past. I am using human, and We are going to use the antibody for IF next. I am interested in examining cervix carcinoma erythroleukemia as well as myometrium in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
I have checked the website and datasheets of our anti-ZEB1 Monoclonal antibody and I see that M00548 has been validated on human in both WB and IF. Thus M00548 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-11
Question
We are currently using anti-ZEB1 Monoclonal antibody M00548 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The anti-ZEB1 Monoclonal antibody (M00548) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-11
Question
Our lab were content with the WB result of your anti-ZEB1 Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is ZEB1 supposed to be expressed?
S. Banerjee
Verified customer
Asked: 2013-02-28
Answer
Based on literature, cervix carcinoma erythroleukemia does express ZEB1. Generally ZEB1 expresses in nucleus. Regarding which tissues have ZEB1 expression, here are a few articles citing expression in various tissues:
Brain, and Thalamus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2013-02-28
