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Product Info Summary
|Size:||For 5 plates, 96 wells each|
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Human Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Human Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human LGALS1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0762)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: A2-D135
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EZ0762 is reactive to LGALS1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
EZ0762 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EZ0762-CA||Rabbit anti- human LGALS1 polyclonal antibody (Capture Antibody)||500 µl, 0.4 mg/mL (recommended dilution 1:100)||0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol|
|EZ0762-DA||Biotinylated goat anti- human LGALS1 polyclonal antibody (Detection Antibody)||500 µl, 5 µg/mL (recommended dilution 1:100)||0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol|
|EZ0762-ST||Lyophilized recombinant human LGALS1 standard||10 ng/tube×2|
|AR1103||Avidin-Biotin-Peroxidase Complex (ABC)||500 µl (recommended dilution 1:100)|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. 96 well microplate (Cat# AR1100)
- 6. Plate Sealers.
- 7. Capture Antibody Diluent: PBS.
- 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 11. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Galectin-1 EZ Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
Gene/Protein Information For LGALS1 (Source: Uniprot.Org, NCBI)
14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LGALS1, check out the LGALS1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LGALS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EZ0762 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of immune checkpoint molecules in endometrial carcinoma
MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma
Tang D, Yuan Z, Xue X, Lu Z, Zhang Y, Wang H, Chen M, An Y, Wei J, Zhu Y, Miao Y, Jiang K. Int J Cancer. 2012 May 15;130(10):2337-48. Doi: 10.1002/Ijc.26290. Epub 2011 Nov 28. High Expression Of Galectin-1 In Pancreatic Stellate Cells Plays A Role...
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