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Product Info Summary
| SKU: | A07719-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZIP7 SLC39A7 Antibody
SKU/Catalog Number
A07719-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZIP7 SLC39A7 Antibody (Catalog # A07719-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZIP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZIP7 SLC39A7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07719-2)
Host
Rabbit
Contents
ZIP7 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-ZIP7 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human ZIP7. The immunogen is located within amino acids 20-70 of ZIP7.
Reactive Species
A07719-2 is reactive to SLC39A7 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
50.1 kDa
Background of SLC39A7
The zinc transporter ZIP7, also known as SLC39A7, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP7 was initially identified while characterizing genes in the major histocompatibility complex on chromosome 17. ZIP7 mRNA is abundantly and widely expressed and the protein localizes to the Golgi apparatus. It functions to transport intracellular zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 expression is expressed by zinc. ZIP7 has been suggested to act a hub for tyrosine kinase activation and may thus be a potential therapeutic target for diseases such as cancer where prevention of tyrosine kinase activation would be advantageous.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07719-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-1 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: ZIP7, A07719-2 (1 μg/mL), ZIP7, 24-029 (4 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: ZIP7, A07719-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: ZIP7, A07719-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse Brain Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: ZIP7, A07719-2 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Rat Brain Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: ZIP7, A07719-2 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of ZIP7 in Human Brain Tissue
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ZIP7 antibody (A07719-2) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of ZIP7 in Human Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling ZIP7 with A07719-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Specific Publications For Anti-ZIP7 SLC39A7 Antibody (A07719-2)
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