Acyl-coenzyme A thioesterase 1 (Acot1)

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. True acyl-CoA thioesterase being highly specific for saturated C12-C20 acyl-CoAs, with only a very low action with decanoyl-CoA as substrate.

Most active on myristoyl- and - palmitoyl-CoA. Introduction of a single or two double bonds decreases the activity to approximately half.