Acot1 Antibodies

Acyl-coenzyme A thioesterase 1 (Acot1)

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. True acyl-CoA thioesterase being highly specific for saturated C12-C20 acyl-CoAs, with only a very low action with decanoyl-CoA as substrate.

Most active on myristoyl- and - palmitoyl-CoA. Introduction of a single or two double bonds decreases the activity to approximately half.

Gene Information

Mouse
Gene Name:	Acot1
Uniprot:	O55137
Entrez:	26897

Family

Belongs to:
C/M/P thioester hydrolase family

Alternative Names

ACH2; acyl-CoA thioesterase 1Long chain acyl-CoA thioester hydrolase; acyl-coenzyme A thioesterase 1; CTE1; CTE-1; CTE-I; CTE-Ib; EC 3.1.2.2; Inducible cytosolic acyl-coenzyme A thioester hydrolase; LACH2; Long chain acyl-CoA hydrolase

Molecular Weight

Mass (kDA):
46.136 kDA

Genomic Context

Mouse
Location:	12 D1|12 39.0 cM
Sequence:	12;

Tissue Specificity

Expressed in heart, kidney, brown adipose tissue, white adipose tissue, adrenal gland and muscle.

Diseases

• Cell Transformation, Neoplastic
• Cardiomyopathies
• Maternal Exposure
• Obesity
• Malignant Paraganglionic Neoplasm
• Diabetes Mellitus
• Hypoglycemia

• Diabetic Cardiomyopathies
• Prostatic Diseases

Pathways

• Fatty Acid Oxidation
• Lactation
• Insulin Secretion
• Cell Differentiation
• Fatty Acid Beta-oxidation
• Autophagy

• Secretion

PTMs

• Oxidation

• Carboxylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Acot1

Boster Bio - Best Uses Of The ACOT1 Marker

This article introduces the ACOT1 marker and its target and functions. It was created to help researchers understand ACOT1 as well as the various ways it is used. You can also use the gene search bar in order to find any gene you are interested. Boster Bio: The ACOT1 Marker's Best Uses

Acyl-Coenzyme A thioesterase 1 (Acot1)

The ACOT1 marker has been used in many studies to detect antioxidant status and protect cells against cytotoxic agents. Many people are unaware of the many uses this gene has. This article discusses the various uses of the ACOT1 gene marker. Continue reading to learn about the many uses of the ACOT1 gene marker. Listed below are the best uses of the ACOT1 gene marker. This is an important protein to protect cells from oxidative Stress.

The ACOT1 marker can be used to detect the AC-ATPase enzyme. It is involved in fatty acid metabolic, converting fattyacyl-CoAs and CoA-SH to free fatty acids. It is essential for the efficient functioning cardiac myocytes, as they have large mitochondria that can be oxidized. Acot1 is believed that it reduces oxidative stresses in cardiomyocytes. This protects heart function in many diseases.

Dox treatment significantly changed gene expression in the murine's heart. A change in Acot1 following DOX treatment was confirmed by Western Blot. The researchers then did a Protein-Protein Interaction, (PPI), network analysis to determine which proteins are involved within the DOX-related pathways. KEGG pathway enrichment analysis revealed that Acot1 has a role in the biosynthesis of unsaturated fatty acids.

Function

ACOT1/2 & ACOT11 markers were expressed in lower amounts in ccRCC compared to matched normal renal samples. These markers were identified as a promising diagnostic biomarker in ccRCC. This research raised concerns about their possible roles in ccRCC progression and tumorigenesis. This study investigated the possible roles of these genes as well as their expression in ccRCC utilizing the TCGA database.

The TCGA KIRC dataset was used to analyze the ACOT8 network of co-expression genes. The ACOT8 gene cluster exhibited increased expression compared to the other two markers. The analysis also revealed a possible role for ACOT1 as a lung cancer treatment. Although ACOT1/2/11/13 expression was not associated with ccRCC growth, ACOT8 expression correlated with tumor growth.

The ACOT1 Gene may also play an important role in lipid metabolism. Studies have shown ACOT1 is linked to oxidative capacity as well as PPARa activities. Its role is unclear in lipid metabolism. It is known to be involved PPARa signaling as well as FA flux, which are critical for the regulation PPARa. Poor ACOT1 function is known to cause inflammation.

In a TCGA KIRC dataset, ACOT1/2-8, 13 and 13 expression levels of ccRCC genes were also identified. ACOT1 was significantly associated to overall survival, but ACOT2/11/13 showed no correlation with the development of the tumor. The data suggest that ACOT8/ACOT11 play discordant roles during ccRCC tumorigenesis, progression.

Several tissues express Acot1 gene, which is highly regulated at both the mRNA and protein levels. It is found in many human tissues, including brain and heart, lung, kidney, and kidney. The Acot1 gene can be quickly controlled at both the protein level and the mRNA level. It is important to examine the role of Acot1 gene in various diseases. It is an essential gene that regulates cell growth and development.

ACOT1 is a central regulator in fatty acid metabolic. Similarly, it controls intracellular levels of PPARa and HNF4a ligands. The PPARa gene could play a positive role in regulating ACOT1, and upregulation may provide the ligand necessary for PPARa signaling. ACOT1 may also be a mechanism that induces PPARa to continue upregulation.

Target

Recently, researchers discovered a drug which targets the ACOT1 marker in Boster Bio. The compound inhibits PPARa and promotes oxidative potential in a cell experiment. Although ACOT1's function is still not fully understood, it may play a role in FA trafficking during an increase in hepatic FA Infusion. It is possible to target this marker in a cell model that mimics inflammation for therapeutic purposes.

Hypotheses have been made regarding the mechanism of ACOT1's effect on PPARa expression in cancer cells. ACOT1 may be a cytosolic proteins with multiple PKA-phosphorylation sites. Therefore, its nuclear localization might result from elevated cAMP/PKA signaling during fasting. To test this hypothesis we treated AML12 with a PKA inhibition and 8Br-cAMP. The H89 PKA inhibitor blocked the cAMP-induced translocation ACOT1 from cytosol to nucleus. As a consequence, nuclear ACOT1 levels were reduced.

After transfection with an anti-ACOT1 polyclonal antibody, the protein was mixed with a buffer containing 8%, 6 mM blue bromophenol, and 4.3 mg glycerol. After this, 30ug of protein were transferred onto Mini-PROTEANTM TGX stain-free precast gels. The gels were stained by an HRP conjugated secondary antibody. The secondary antibodies were then used to detect the primary antibodies.

The results also show that knocking out ACOT1 regulates FA flux in fasted hepatocytes. Acot1 knockdown increases FA oxidation within hepatic cells. These results indicate that ACOT1 knockdown increases oxidative capacity. Boster bio has been identified as the ACOT1 target. Once again, we've found a drug that targets the ACOT1 marker in Boster bio and its metabolite.

Applications

The human ACOT1 gene can be found in the genome at the UCSC Genome Browser. UniProtQ86TX2. The gene can also been found at PDBeKB. This article is based on text from the United States National Library of Medicine, which is publicly available. It also includes the ACOT1 marker. ACOTs play a role in familial hypercholanemia inheritance, as well as in the metabolism of acyl-CoAs.

ACOT1 is one of several human genes that participate in lipid metabolism. It is found in a group of genes on human 14q24.3. Its genes are called ACOT1,ACOT2,ACOT4,and ACOT6. ACOT1 is present in the cytosol of human skin fibroblasts. It is essential to peroxisomes' function in cellular metabolism.

The short form of ACOT6 is found in the human ACOT1 gene. The human ACOT1 gene contains the short form of the enzyme, which is then converted to an active enzyme. The ACOT1 human short form has a lower specificity.

The human ACOT1 gene is composed of two distinct domains - the N-terminal domain and the C-terminal domain. The N-terminal domain contains a seven-stranded b-sandwitch with three short antiparallel strands and four longer ones. The central b-strands contain a catalytic triad - Ser232, His360, and Asp326 - located near the C-terminal end of the b-strands.

The ACOT1 gene is responsible for long-chain acylcoA thioesterase activity within human peroxisomes. The thioesterase has been shown that it can influence FA oxidation. ACOT1 expression in high levels increases PPARa reporter activities in human cells. The ACOT1 genes were also important in the regulation of liver disease-related hepatic metabolism.

ACOT8 is a vital auxiliary enzyme found within peroxisomes. Human peroxisomes contain two ACOT1s and mice have six. They also contain one N-acyltransferase of bile acid (BAAT), as well as three acyltransferases. These genes are not necessary for human health, but they play a part in the metabolism fatty acids.