Cbx2 Antibodies

Chromobox protein homolog 2 (Cbx2)

Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to keep the transcriptionally repressive state of many genes, including Hox genes, during development (By similarity). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A'Lys-119', rendering chromatin heritably changed in its expressibility (By similarity).

Binds to histone H3 trimethylated in'Lys-9' (H3K9me3) or at'Lys- 27' (H3K27me3) (PubMed:16537902). Plays a role in the lineage differentiation of the germ layers in embryonic development (PubMed:22226355).

Gene Information

Mouse
Gene Name:	Cbx2
Uniprot:	P30658
Entrez:	12416

Family

Belongs to:
No superfamily

Alternative Names

Cbx2; CDCA6; cell division cycle associated 6; chromobox homolog 2 (Drosophila Pc class); chromobox homolog 2 (Pc class homolog, Drosophila); chromobox homolog 2; chromobox protein homolog 2; M33; MGC10561; modifier 3; Pc class homolog; SRXY5

Molecular Weight

Mass (kDA):
54.918 kDA

Genomic Context

Mouse
Location:	11 E2|11 83.33 cM
Sequence:	11;

Tissue Specificity

Expressed in embryoid bodies.

Diseases

• Malignant Neoplasms
• Carcinoma
• Embryonal Carcinoma
• Disorders Of Sex Development
• Neoplasms
• Tumor Progression
• Malignant Neoplasm Of Breast
• Teratogenic Effect
• Osteosarcoma
• Nervousness
• Mammary Neoplasms
• Chimera Disorder

• Bipolar Disorder
• Liver Regeneration Disorder
• Mammary Neoplasms, Experimental
• Cell Transformation, Neoplastic
• Anemia
• Genomic Instability
• Malignant Paraganglionic Neoplasm

Pathways

• Localization
• Metaphase
• Gene Silencing
• Sex Determination
• Interphase
• Cell Proliferation
• Heterochromatin Assembly
• Protein Targeting
• Histone Modification
• Nuclear Export
• Leydig Cell Differentiation
• Cell Maturation
• Cell Cycle

• Prophase
• Cell Differentiation
• Chromatin Organization
• Dna Methylation
• Segment Specification
• Larval Development
• Methylation

PTMs

• Methylation

• Phosphorylation

• Dephosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Cbx2

Best Uses of the CBX2 Marker in Boster Bio

The CBX2 gene has many applications. Boster scientists may submit results for specific species, applications, or special samples. Researchers who submit the results of a specific species or for an application can earn product credits. This program is open to scientists from all over the world. Listed below are some of the best uses for the CBX2 marker. For more information, please visit Boster Bio's website.

Positive

There are many positive applications of the CBX2 gene marker, but they are not widely known. The marker has been shown in tumorigenesis. It is also known that it promotes invasion and growth of glioma cellular cells via the Akt/PI3K pathway. However, more research is needed in order to understand the mechanism. Here are the positive uses of the CBX2 marker.

It is known that the expression of CBX2 is correlated with the protein levels in TCGA breast tumor samples. It is associated with an over- or under-expressed proportion of tumors. This marker appears to be linked with metabolic functions in breast tumour cells. This could make it a diagnostic tool. CBX2 could be used in aggressive breast carcinoma treatment, as well as its usefulness in identifying cancerous tumors.

In cell proliferation experiments, the positive effects knockdown CBX2 had also been shown to be effective. CBX2-transfected cells were then seeded in triplicate in six-well plates and incubated at 37°C for 2 weeks. The colonies were then cleaned, fixed with 4% Paraformaldehyde for 20 minutes, and stained with 0.2% Crystal Violet for 30 min. Positive colonies were those with at least 50 cells. They were then counted using a microscope.

Other researchers have confirmed that the CBX2 protein is related to EZH2; it has an oncogenic role, which is associated with a poor prognosis when it comes to breast cancer. CRC may be prognosticated by the CBX2 Gene. The Hippo pathway regulates CBX2 gene expression. This pathway controls genes related to proliferation, apoptosis, and other functions.

Negative

Previous studies have demonstrated positive and adverse effects of the CBX2 gene in boster Bio. This marker has been found in both boster and in human cells. It is useful in diagnosing cancer. Some methods use a monoubiquitination test of H3K4me3 whereas others use a more thorough chromatin modification test. The presence or absence of methylated or non-methylated forms of the gene can help identify the PRC1 version.

The complex consists of the CBX2 heterodimer and the RAWUL box of PCGF2. CBX2 also includes the RING1B/PCGF2 heteromer and CBX2. Similar activity is found in RING1B–PCGF2 heteromer and CBX2. This interaction could be useful for the treatment or prevention of prostate tumors.

Optimized

The versatile CBX2 genetic marker can be used in gene therapy and cell-based cell development. The CBX2 gene has many uses, but it is rarely used for a specific purpose. This marker can be used in cell culture studies to help distinguish between different types of cancer. The CBX2 gene is located in the cleft palate, the region between the two halves of the brain.

Primary antibody with high affinity

CBX2 was ubiquitinated using a high affinity primary antibody. This protein is essential to cell signalling in multiple tissues and acts as a scaffold of complex RNA synthesis and RNA synthesis. However, CBX2 was reduced by SUMO2/3 knockdown and promoted its destruction.

The Polycomb Group (PcG), proteins that regulate CBX2 are critical regulators in PcG-mediating Activity. Recent research has established the role of CBX2 for human cancers. CBX2 expression is high in several human cancers including prostate cancer. CBX2's Chromodomain has been associated with overall survival and tumorigenesis. Depleting CBX2 from prostate cancer cells in humans negatively impacts the proliferation of tumors.

The three K/R CBX2 mutations significantly reduce cell viability. This mutation was recognized by SAHA (a molecule that promotes CBX2 protein degradation). It also inhibits the activity Ubiquitin ligase (ubiquitin like protein-binding ligand-A).

The regulation of stemness in leukemic tissues and self-renewal is part of the CBX2 gene. CBX2 instability may also play a role, allowing for chromatin reset at certain loci. This may apply to leukemias affected by chromatin. Targeted strategies for leukemic patients with CBX2 mutants could prove to be more effective and cost-effective than current therapies.

Zeta Corporation has developed highly sensitive and specific primary antibodies using a high quality protein chip. MonoMAb(tm), FFPE tissue-section-validated antibodies. Zeta Corporation’s RAbMono(tm), Rabbit monoclonal antibodies stand out for their high level of specificity. The company is based out of Southern California and holds ISO 13485, FDA, and ISO 13485 certifications.

Applications

The CBX2 marker is a highly sensitive biomarker that is present in many cancers. The marker is expressed in many tissues, including the brain and skin. Its expression can be correlated to the presence of CBXregulated genes, which allows the precise determination of those genes. This marker has many applications in the field cancer research.

Numerous studies have shown that the CBX2 gene could have therapeutic implications for oesophageal-squamous cell carcinoma. In this study, we examined the expression of the gene using real-time quantitative reverse-transcription-PCR and immunohistochemistry. OSCC cell lines expressing CBX2 displayed co-ordinated expression with the WNT5B gene. Additionally, OSCC cells with high CBX2 levels showed shorter survival rates for disease-specific and overall conditions, as well a lower rate of hematogenous events.

Previous studies have shown CBX2 plays an important role in the development the male urogenital tract. It has also been shown that it plays a role in prostatic differentiation and proliferation. It has been shown to promote drug resistance by being involved in the aberrant PcG mediated silencing PCa-related genes. These findings are exciting news for cancer research, especially in tumorigenesis.

CBX2 was first discovered in a medaka fish. Its conservation in vertebrates was shown by phosphorylation within the protein's serinerich region. As humans evolved from fish to fish, the serine rich region in CBX2 dropped from twenty-four residues to sixteen. This protein also contains a H3K27me3 silencing marker, and recruits PRC1 for gene regulation. Its regulation of cell division is a hallmark feature of human CRPC.