NEDD4L Antibodies

AND NEDD4L ELISA kits, NEDD4L Proteins

Many biological assays use antibodies to detect NEDD4L, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with NEDD4L in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop NEDD4L antibodies...

We validate the specificity of these antibodies to NEDD4L by testing them on tissues known to express NEDD4L positively and negatively. Browse below to find the NEDD4L antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for NEDD4L? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the NEDD4L Western Blot Guide

NEDD4L Infographic by Boster Bio

All NEDD4L Products

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Specificity

Applications

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Anti-NEDD4-2/NEDD4L Antibody Picoband®

1 Citations

Western blot analysis of NEDD4-2 using anti-NEDD4-2 antibody (A01595-1)

Flow Cytometry analysis of U20S cells using anti-NEDD4-2 antibody (A01595-1)

Human, Mouse, Rat

ELISA, Flow Cytometry, IHC, ICC, WB

$370
Cat # A01595-1
100 μg/vial

Anti-NEDD4-2 NEDD4L Rabbit Monoclonal Antibody

Western blot analysis of NEDD4-2 expression in mouse spleen tissue

Western blot analysis of NEDD4L using anti-NEDD4L antibody (M01595)

Human, Mouse, Rat

IP, WB

$370
Cat # M01595
100 μl

NEDD4L Overview

Facts about E3 ubiquitin-protein ligase NEDD4-like.

NEDD4L 3D structure. Source: alphafold

E3 ubiquitin-protein ligase NEDD4-like (NEDD4L)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation.

Promotes ubiquitination and internalization of different plasma membrane channels like ENaC, SCN2A/Nav1.2, SCN3A/Nav1.

Gene Information

Human
Gene Name:	NEDD4L
Uniprot:	Q96PU5
Entrez:	23327

Family

Belongs to:

No superfamily

Alternative Names

E3 ubiquitin-protein ligase NEDD4-like; EC 6.3.2; EC 6.3.2.-; FLJ33870; hNedd4-2; KIAA0439ubiquitin-protein ligase NEDD4-like; NEDD4.2; NEDD4-2; NEDL3; neural precursor cell expressed, developmentally down-regulated 4-like; neural precursor cell expressed, developmentally down-regulated gene 4-like; RSP5ubiquitin-protein ligase Rsp5

Molecular Weight

Mass (kDA):
111.932 kDA

Genomic Context


Human

Location:	18q21.31
Sequence:	18; NC_000018.10 (58044226..58401540)

Tissue Specificity

Ubiquitously expressed, with highest levels in prostate, pancreas and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597). Expressed in melanocytes (PubMed:23999003).

Subcellular Localizations

Cytoplasm. Golgi apparatus. Endosome, multivesicular body. May be recruited to exosomes by NDFIP1.

Diseases

Hypertensive Disease
Essential Hypertension
Malignant Neoplasms
Neoplasms
Prostatic Neoplasms
Hypotension Adverse Event
Malignant Neoplasm Of Prostate
Dysequilibrium Syndrome
Malignant Paraganglionic Neoplasm
Liddle Syndrome
Carcinogenesis
Cell Invasion
Hyperplasia

Neoplasm Metastasis
Coronary Heart Disease
Diabetic Nephropathy
Metastatic Malignant Neoplasm To The Lymph Node
Developmental Reading Disorder
Trinucleotide Repeat Expansion
Glioma

Interacting Proteins

DAZAP2
SLC11A2
SMAD7

Pathways

Pathogenesis
Transport
Cell Growth
Excretion
Intracellular Transport
Ion Transport
Endocytosis
Conjugation
Localization

Cytokinesis
Regeneration
Exocytosis
Rna Interference

PTMs

Ubiquitination

Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NEDD4L

References

PMID: 11840194 by Chen H., et al. NEDD4L on human chromosome 18q21 has multiple forms of transcripts and is a homologue of the mouse Nedd4-2 gene.

PMID: 14556380 by Malbert-Colas L., et al. Identification of new partners of the epithelial sodium channel alpha subunit.