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- Table of Contents
Facts about Oxysterol-binding protein 1.
Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase that dephosphorylates ERK1/2, whereas 25-hydroxycholesterol induces its disassembly (PubMed:15746430). Regulates cholesterol efflux by decreasing ABCA1 equilibrium (PubMed:18450749).
Human | |
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Gene Name: | OSBP |
Uniprot: | P22059 |
Entrez: | 5007 |
Belongs to: |
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OSBP family |
OSBP1oxysterol-binding protein 1; oxysterol binding protein
Mass (kDA):
89.421 kDA
Human | |
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Location: | 11q12.1 |
Sequence: | 11; NC_000011.10 (59574398..59615774, complement) |
Widely expressed.
Cytoplasm, cytosol. Cytoplasm, perinuclear region. Golgi apparatus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network. Predominantly cytosolic.
Oxysterol-binding protein (OSBP) is a lipid transporter in mammals that exchanges sterol for phosphatidyl 4-phosphate. This protein is also involved with cholesterol metabolism, binding to the oxysterols. Biological assays employ monoclonal and polyclonal antibodies against OSBP. Boster Bio uses mouse (rat) and rabbit (mice) as models organisms for OSBP antibody research.
The sterol binding protein family includes oxygenysterol binding protein (OSBP). It is believed to regulate a range of sterol dependent cellular processes, including cholesterol metabolic. It is still not clear what it does inside the body. OSBP is not only involved in cholesterol metabolism but may also play an important part in the development Alzheimer's.
Oxysterol-binding protein (OSBP) is a gene that shuttles lipids between intracellular membranes. It is also involved in the regulation of cholesterol and oxysterol signals. It is essential for understanding cellular homeostasis. Boster Bio's Oxysterol binding protein marker could help in these determinations.
The Oxysterol-binding protein marker is made from a DNA sequence and expressed in E. coli. It is purified to greater than 90% purity. It is a product for life science research and is guaranteed for 2 years if stored at -70C. Boster Bio's Oxysterolbinding protein marker makes lipid binding enzyme research easier.
Flow cytometry is a powerful technique that analyzes cellular interactions. It has many applications in science and medicine. The sample can consist of cells or particles. Boster Bio offers high-affinity primary antibodies. They can detect both monoclonal (and polyclonal) proteins in flow cytometry. They are well-known and have been used for over 25 years.
High-affinity antibody are required to improve sensitivity and detect the target correctly. Boster Bio high-affinity antibodies have been developed in an innovative plasma-cell discovery process. In this process, individual cells treated with proprietary chemistry are screened. These cells stop B cells from secreting antibody and keep antibodies on cells membranes. Then, these cells are incubated with flurochrome-conjugated antigens and plasma cells to detect the antibodies.
Polyclonal antibodies are acceptable for research and can be used to guide the development of monoclonal. Polyclonal antibodies can be used to detect secretive proteins and biomarkers, as most antigens produce both types of antibodies. Additionally, polyclonal antibody are inexpensive, simple to make, and can often be produced quickly. For researchers, this is a great option because Boster Bio can produce polyclonal antibodies for under $600. The downside to polyclonal antibody is that they can vary greatly from lot-to-lot and are therefore not suitable for clinical assays.
Boster Bio high affinity primary antibodies provide specificity for protein identification. Researchers can also use multiple controls to validate their research. This method is known as immunoassay, and uses antibodies with varying degrees of specificity to detect proteins. Scientists can thus determine if their primary antibody is specific to a target protein. Multiple bands are a sign that the antibody has been compared against that protein and is a good match in a specific assay.
The OSBP Marker is a new gene that interacts genetically with the fan, the precursor of ceramide transport. It recruits proteins to ER-Golgi. This article will cover the function and applications OSBP in cells. We will also discuss how OSBP interacts to the fan. This work is in its initial stages, but it will grow over time. Here are some examples.
The OSBP Marker has five functional regions: the N-terminal intrinsically disordered region, the PH domain, the putative central CC domain, and the FFAT motif. These five domains comprise the protein. They bridge the ER, the TGN. The NPH-FFAT construct resembles this tethering function, however it lacks ORD. OSBP is the acronym for the purified protein.
The OSBP Marker allows us to examine the role of OSBP during membrane remodeling. OSBP facilitates two lipid-transfer regions between membranes by acting as a mediator of sterol transportation. To function properly, membrane remodeling requires multiple OSBP molecular interactions. The OSBP Marker is crucial in determining whether these molecules are involved in a specific pathway. OSBP Marker can be used in many cellular processes. It has many medical, pharmaceutical, and biological applications.
OSBP has been proven to be an effective marker of ER–Golgi contact points. The loss or reduction of the protein causes an increase in PI-4P, which is primarily catabolic. Sac1 reduces the PI-4P amount in cells without OSBP. It is possible that residual OSBP could function to catabolize Pi-4P.
The OSBP Marker is able to reduce intermembrane differences by facilitating transfer of OSBP molecular between two membranes. OSBPs can facilitate cross-border activities which improve security and reduce corruption. This marker can be used to improve the service provided by border officials. It will result in increased security, safety and efficiency. Passenger dwell time will also be reduced. It will also speed the clearance process.
The history of Steven Boster begins in 1993, when he developed his first product. The company quickly became China's largest catalog anti-body company and Steve was known as "the lavatory genius". He was responsible for the development of hundreds of primary antibodies for use in immunohistochemistry, molecular biology, and immunohistochemistry. Boster later invented PicoKine(tm), a proprietary ELISA platform that allowed for the creation of high-sensitivity ELISA kits.
Steve Boster, a Joliet, IL native, died June 6, 2022 following a battle with COVID-19. He was the father to Donald, Sr., David Boster, Nina Mae Hall. His mother Frances Boster of Verona, WI and his sisters Kimberly Blanton & Tammy Boster, along with his son Jonathan in Herrin IL, are his survivors.
PMID: 1970801 by Levanon D., et al. cDNA cloning of human oxysterol-binding protein and localization of the gene to human chromosome 11 and mouse chromosome 19.
PMID: 11278871 by Moreira E.F., et al. Molecular and biochemical characterization of a novel oxysterol- binding protein (OSBP2) highly expressed in retina.