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Product Info Summary
| SKU | AR1160 |
|---|---|
| Product name | Cell Counting Kit-8 |
| Pack size | 5 mL (for 500 assays, 10μL per well) |
Product info
Product Overview
| Form | Liquid |
|---|---|
| Size | 5 mL (for 500 assays, 10μL per well) |
| Concentration | 6mg/ml |
| Buffer | 9% NaCl |
| Storage | Upon receipt store at -4°C, protect from light. Note: Repeated thawing and freezing causes an increase in the background, which interferes with the assay. |
| Stability | When stored as directed, product should be stable for one year. Store it at -20˚C for longer storage |
| Sensitivity | CCK-8 is the highest sensitive dye for the cell based assay. |
| Equivalent | Dojindo Molecular Technologies (Product No. CK04);
Millipore Sigma (Product No. 96992) |
Introduction
Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt. WST-8 [2- (2- methoxy-4-nitrophenyl)-3- (4-nitrophenyl)-5- (2, 4-disulfophenyl)-2H- tetrazolium, monosodium salt]* produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. Cell Counting Kit-8 is a one-bottle solution; no premixing of components is required. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The cell proliferation assay using CCK-8 correlates well with the [ (3)H] thymidine incorporation assay. And the CCK-8 assay can also be substituted for the [ (3)H]thymidine incorporation assay. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.
Advantages
1. One bottle, ready to use solution.
2. No organic solvents or isotopes required
3. No harvesting, no washing and no solubilization steps.
4. No Toxicity to Cell
5. More sensitive than MTT, XTT, MTS or WST-1
Precautions
1. Run pilot test to determine the optimal cell number and incubation time.
2. Since the CCK-8 assay is based on the dehydrogenase activity detection in viable cells, conditions or chemicals that affect dehydrogenase activity in viable cells may cause discrepancy between the actual viable cell number and the cell number determined using the CCK-8 assay.
3. WST-8 may react with reducing agents to generate WST-8 formazan. Please check the background O.D. if reducing agents are used in cytotoxicity assays or cell proliferation assays.
4. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.
5. Phenol red containing culture media can be used with this kit for cell viability assays.
6. Membrane filtration is recommended for the sterilization of the CCK-8 solution, if necessary.
7.The incubation time varies by the type and number of cells in a well. Generally, leukocytes give weak coloration, thus a long incubation time (up to 4 hours) or a large number of cells (~105 cells/well) may be necessary.
8. Since the cytotoxicity of this kit is very low, further color development is possible after reading the absorbance.
9.Neutral red or crystal violet can be used after the CCK-8 assay.
10.Measure the reference wavelength at 600 nm or higher if there is a high turbidity in the cell suspension.
Additional Materials Required
10μL and 100-200μL Multichannel Pipettes
Plate reader (450 nm filter)
96-well plate
CO2 incubator
General Protocol
Cell Number Determination
1. Inoculate cell suspension (100μL/well) in a 96-well plate. Pre-incubate the plate in a humidified incubator (e.g., at 37 ˚C, 5% CO2).
2. Add 10μL of the CCK-8 solution to each well of the plate. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.
3. Incubate the plate for 1-4 hours in the incubator.
4.Measure the absorbance at 450 nm using a microplate reader. To measure the absorbance later, add 10μL of 1% w/v SDS or 0.1M HCl to each well, cover the plate and store it with protection from light at room temperature. No absorbance change should be observed for 24 hours.
Cell proliferation and Cytotoxicity Assay
1. Dispense 100μL of cell suspension (5000 cells/well) in a 96- well plate. Pre-incubate the plate for 24 hours in a humidified incubator (e.g., at 37 ˚C, 5% CO2).
2. Add 10μL of various concentrations of substances to be tested to the plate.
3. Incubate the plate for an appropriate length of time (e.g., 6, 12, 24 or 48 hours) in the incubator.
4. Add 10μL of CCK-8 solution to each well of the plate. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.
5. Incubate the plate for 1-4 hours in the incubator.
6. Measure the absorbance at 450 nm using a microplate reader. To measure the absorbance later, add 10μL of 1% w/v SDS or 0.1M HCl to each well, cover the plate and store it with protection from light at room temperature. No absorbance change should be observed for 24 hours.
Frequently Asked Questions
1. How many cells should there be in a well?
For adhesive cells, at least 1000 cells are necessary per well (100μL medium). For leukocytes, at least 2500 cells are necessary per well (100μL medium) because of low sensitivity. The recommended maximum number of cells per well for the 96-well plate is 25000. If a 24 well or 6 well plate is used for this assay, please calculate the number of cells per well accordingly, and adjust the volume of the CCK-8 solution in a well to 10% of the total volume.
2. Does CCK-8 stain viable cells?
No. Since WST-8 and its formazan dye are highly water-soluble, CCK-8 cannot be utilized for cell staining purpose.
3. Does phenol red affect the assay?
No. The absorption value of phenol red in a culture medium can be removed by subtracting the absorption value of a blank solution from the absorption value of each well. Therefore, a medium containing phenol red is usable for the CCK-8 assay.
4. Is CCK-8 toxic to cells?
Since the toxicity of CCK-8 is so low, the same cells can be used for other cell proliferation assays such as the crystal violet assay, neutral red assay or DNA fluorometric assay after the CCK-8 assay is completed.
5. I do not have a 450 nm filter. What other filters can I use?
You can use filters with the absorbance between 430 and 490 nm, even though 450 nm filter gives the best sensitivity.
Images
Product Images
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Figure 1. Absorption spectrum of WST-8 formazan
Figure 1 shows the absorption spectrum of WST-8 formazan. Since the absorbance at 460 nm is proportional to the number of viable cells in the medium, the viable cell number can be determined using the absorbance of a previously prepared calibration curve.
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Figure 2. Cell proliferation assay using CCK-8
Culture medium: MEM, 10% FBS
Incubation: 37 ℃, 5% CO2, 2 hours
Deteactin: 450nm
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Figure 3. Toxicological test of chemicals using CCK-8
Cell line: hela
Medium: DMEM, 10% FBS Chemicals: 200 μM Cisplatin (DDP)
Incubation: 37°C, 5% CO2, 2 hours
Specific Publications For Cell Counting Kit-8 (CCK-8) (AR1160)
AR1160 has been cited in 129 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The osteogenesis of bacterial cellulose scaffold loaded with bone morphogenetic protein-2
Fabrication of polylysine based antibacterial coating for catheters by facile electrostatic interaction
CD73/NT5E is a target of miR-30a-5p and plays an important role in the pathogenesis of non-small cell lung cancer
KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway
C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation
Polyoxometalate-Based Radiosensitization Platform for Treating Hypoxic Tumors by Attenuating Radioresistance and Enhancing Radiation Response
Clara cell 10-kDa protein inhibits TH17 responses through modulating dendritic cells in the setting of allergic rhinitis
Artificially Engineered Cubic Iron Oxide Nanoparticle as a High-Performance Magnetic Particle Imaging Tracer for Stem Cell Tracking
Lipocalin2 suppresses metastasis of colorectal cancer by attenuating NF-κB-dependent activation of snail and epithelial mesenchymal transition
Self‐Healing Silk Fibroin‐Based Hydrogel for Bone Regeneration: Dynamic Metal‐Ligand Self‐Assembly Approach
Yao X,Jing X,Guo J,Sun K,Deng Y,Zhang Y,Guo F,Ye Y.Icariin Protects Bone Marrow Mesenchymal Stem Cells Against Iron Overload Induced Dysfunction Through Mitochondrial Fusion and Fission, PI3K/AKT/mTOR and MAPK Pathways.Front Pharmacol.2019 Feb 28;10:163. doi:10.3389/fphar.2019.00163.PMID:30873034;PMCID:PMC6403125.
Species: Rat
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:BMSCS, DILUTION:NA
Li Q,Zong Y,Li K,Jie X,Hong J,Zhou X,Wu B,Li Z,Zhang S,Wu G,Meng R. Involvement of endothelial CK2 in the radiation induced perivascular resistant niche (PVRN) and the induction of radioresistance for non-small cell lung cancer (NSCLC) cells.Biol Res.2019 Apr 16;52(1):22. doi:10.1186/s40659-019-0231-x.PMID:30992075;PMCID:PMC6466699.
Species: Human
AR1160 usage in article: APP:CCK-8 ASSAY, SAMPLE:HUVECS, DILUTION:NA
Qu Y,Dou P,Hu M,Xu J,Xia W,Sun H.circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis.Mol Med Rep.2019 Apr;19(4):3314-3320.doi:10.3892/mmr.2019.9965.Epub 2019 Feb 18.PMID:30816475.
Species: Human
AR1160 usage in article: APP:CELL PROLIFERATION ASSAY, SAMPLE:MCF-7 CELL AND ZR-75-30 CELL, DILUTION:NA
Yao X,Zhang J,Jing X,Ye Y,Guo J,Sun K,Guo F.Fibroblast growth factor 18 exerts anti-osteoarthritic effects through PI3K-AKT signaling and mitochondrial fusion and fission.Pharmacol Res.2019 Jan;139:314-324.doi:10.1016/j.phrs.2018.09.026.Epub 2018 Sep 28.PMID:30273654.
Species: Rat
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:CHONDROCYTES, DILUTION:NA
Li Q,Huang P,Chen W,Bi J.Mechanism of bone marrow mesenchymal stem cells secreting miR-26a exosomes affecting high glucose-induced skin fibroblasts function by regulating TLR4/NF-κB signaling.Inflamm Res.2021 Jun 29.doi:10.1007/s00011-021-01478-7.Epub ahead of print.PMID:34185112.
Species: Human
AR1160 usage in article: APP:CELL PROLIFERATION, SAMPLE:BJ CELL, DILUTION:NA
Qiang Wang,Yuan Wang,Feng Zhou,Jie Li,Gang Lu,Yingqian Zhao,"MiR-20a-5p Regulates MPP+-Induced Oxidative Stress and Neuroinflammation in HT22 Cells by Targeting IRF9/NF-κB Axis",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 6621206,9 pages,2021.https://doi.org/10.1155/2021/6621206
Species: Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:HT22 CELL, DILUTION:NA
Chong Liu,Minmin Zhang,Shenyi Ye,Chenliang Hong,Jiaxi Chen,Ruyue Lu,Bingjie Hu,Weijun Yang,Bo Shen,Zhengyi Gu,"Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy",Journal of Immunology Research,vol.2021,Article ID 9979843,12 pages,2021.https://doi.org/10.1155/2021/9979843
Species: Rat
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:H9C2 CELL, DILUTION:NA
Yu L,Huang N,Ge L,Sun H,Fu Y,Liu C,Wang J.Structural design of tetravalent T-cell engaging bispecific antibodies: improve developability by engineering disulfide bonds.J Biol Eng.2021 Jun 29;15(1):18.doi:10.1186/s13036-021-00272-7.PMID:34187511;PMCID:PMC8243740.
Species: Human
AR1160 usage in article: APP:CELL PROLIFERATION ASSAY, SAMPLE:PBMCS, DILUTION:NA
Chao Hu,Xiaobin Zhu,Taogen Zhang,Zhouming Deng,Yuanlong Xie,Feifei Yan,Lin Cai," Tanshinone IIA Inhibits Osteosarcoma Growth through a Src Kinase-Dependent Mechanism",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 5563691,15 pages,2021.https://doi. org/10.1155/2021/5563691
Species: Human,Mouse
AR1160 usage in article: APP:CELL CYCLE ANALYSIS, SAMPLE:U2-OS CELL AND MG-63 CELL, DILUTION:NA
Chen L,Chen H,Ye J,Ge Y,Wang H,Dai E,Ren J,Liu W,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL.Intratumoral expression of interleukin 23 variants using oncolytic vaccinia virus elicit potent antitumor effects on multiple tumor models via tumor microenvironment modulation.Theranostics.2021 May 3;11(14):6668-6681.doi:10.7150/thno.56494.PMID:34093846; PMCID:PMC8171085.
Species: Mouse
AR1160 usage in article: APP:CYTOTOXICITY ASSAY, SAMPLE:B16 CELL AND AB12-LUC CELL, DILUTION:NA
Yuan J,Liu Y,Zhang Q,Ren Z,Li G,Tian R.CircPRDM2 Contributes to Doxorubicin Resistance of Osteosarcoma by Elevating EZH2 via Sponging miR-760.Cancer Manag Res.2021;13: 4433-4445https://doi.org/10.2147/CMAR.S295147
Species: Human,Mouse
AR1160 usage in article: APP:CCK-8 ASSAY, SAMPLE:OS CELL, DILUTION:NA
Fen Chen,Yaru Wu,Jiamin Wu,Hao Zhu,Shuang Chen,Shuaibin Hua,Zixuan He,Changyu Liu,Jun Xiao,Yusheng Shi,Preparation and characterization of ZrO2-Al2O3 bioceramics by stereolithography technology for dental restorations,Additive Manufacturing,2021,102055,ISSN 2214-8604,https://doi.org/10.1016/j.addma.2021.102055.
Species: Rat
AR1160 usage in article: APP:CELL PROLIFERATION, SAMPLE:RBMSCS, DILUTION:NA
Xiaoting Jin,Haiyi Yu,Ze Zhang et al.Surface Charge-Dependent Mitochondrial Response to Similar Intracellular Nanoparticle Contents at Sublethal Dosages,24 May 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-503848/v1]
Species: Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:RAW264.7 CELL, DILUTION:NA
Qian L,Yang X,Li S,Zhao H,Gao Y,Zhao S,Lv X,Zhang X,Li L,Zhai L,Zhou F,Chen B.Reduced O-GlcNAcylation of SNAP-23 promotes cisplatin resistance by inducing exosome secretion in ovarian cancer.Cell Death Discov.2021 May 18;7(1):112.doi:10.1038/s41420-021-00489-x.PMID:34001861.
Species: Human,Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:A2780 CELL AND SKOV3 CELL, DILUTION:NA
Qiu F,Zhang Z,Yang L,Li R,Ma Y.Combined effect of vitamin C and vitamin D3 on intestinal epithelial barrier by regulating Notch signaling pathway.Nutr Metab (Lond).2021 May 8;18(1):49.doi:10.1186/s12986-021-00576-x.PMID:33964955;PMCID:PMC8105975.
Species: Human,Dunkin–Hartley guinea pig
AR1160 usage in article: APP:CELL PROLIFERATION ASSAY, SAMPLE:SW480 CELL, DILUTION:NA
Yang J,Wu L,Liu S,Hu X,Wang Q,Fang L.Long non-coding RNA NEAT1 promotes lipopolysaccharide-induced injury in human tubule epithelial cells by regulating miR-93-5p/TXNIP axis.Med Microbiol Immunol.2021 Apr 22.doi:10.1007/s00430-021-00705-6.Epub ahead of print.PMID:33885954.
Species: Human
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:HK2 CELL, DILUTION:NA
Zhang H,Li M,Zhang J,Shen Y,Gui Q.Exosomal Circ-XIAP Promotes Docetaxel Resistance in Prostate Cancer by Regulating miR-1182/TPD52 Axis.Drug Des Devel Ther.2021 May 3;15:1835-1849.doi:10.2147/DDDT.S300376.PMID:33976535;PMCID:PMC8106459.
Species: Human,Mouse
AR1160 usage in article: APP:CELL COUNTING KIT-8 ASSAY, SAMPLE:PC-3 CELL, DILUTION:NA
Wang Y,Wang W,Yang X,Chen W,Yang X,Pan X,Xu P,Zhu W,Han Y,Chen X.ITGA8 positive cells in the conventional outflow tissue exhibit Schlemm‘s canal endothelial cell properties.Life Sci.2021 May 4:119564.doi:10.1016/j.lfs.2021.119564.Epub ahead of print.PMID:33961857.
Species: Human
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE: HTM CELL, DILUTION:NA
Zhao R,Luo T,Ma P,Ge L,Chen Z,Wang X,Liao W,Bao L.Improvement of the immunogenicity of ESAT-6 via fusion with the dodecameric protein dodecin of Mycobacterium tuberculosis.Microb Pathog.2021 Apr 26;155:104890.doi:10.1016/j.micpath.2021.104890.Epub ahead of print.PMID:33915207.
Species: Mouse
AR1160 usage in article: APP:CELL PROLIFERATION ASSAY, SAMPLE:SPLEEN LYMPHOCYTES, DILUTION:NA
Bian,S.,Zhao,Y.,Li,F.,Lu,S.,He,Z.,Wang,S. ...Wang,J.(2021).Total ginsenosides induce autophagic cell death in cervical cancer cells accompanied by downregulation of bone marrow stromal antigen‑2.Experimental and Therapeutic Medicine,22,667.https://doi.org/10.3892/etm.2021.10099
Species: Human
AR1160 usage in article: APP:CELL VIABILITY, SAMPLE:HELA CELL, DILUTION:NA
Cao Y,Wang Y,Xiao L,Xu JY,Liu Y,Jiang R,Li T,Jiang J.Endothelial-derived exosomes induced by lipopolysaccharide alleviate rat cardiomyocytes injury and apoptosis.Am J Transl Res.2021 Mar 15;13(3):1432-1444.PMID:33841668;PMCID:PMC8014340.
Species: Rat
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:CMS, DILUTION:NA
Pan, A., Li, Y., Guan, J., Zhang, P., Zhang, C., Han, Y. ... Wang, J. (2021). USP18‑deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal‑dependent manner. Oncology Letters, 21, 421. https://doi.org/10.3892/ol. 2021.12682
Species: Human
AR1160 usage in article: APP:CELL PROLIFERATION ASSAY, SAMPLE:HELA CELL, DILUTION:NA
Zhang M, Cheng K. Long non-coding RNA KCNQ1OT1 promotes hydrogen peroxide-induced lens epithelial cell apoptosis and oxidative stress by regulating miR-223-3p/BCL2L2 axis. Exp Eye Res. 2021 Mar 17:108543. doi: 10.1016/j.exer.2021.108543. Epub ahead of print. PMID: 33744257.
Species: Human
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:SRA01/04 CELL, DILUTION:NA
Ji-ling Lv, Li-na Shi, Cong-ying Zhai, Ge-jin Wang, Yan Qu, "Bag-1L Protects against Cell Apoptosis in an In Vitro Model of Lung Ischemia-Reperfusion Injury through the C-Terminal “Bag” Domain", BioMed Research International, vol. 2021, Article ID 8822807, 13 pages, 2021. https://doi.org/10.1155/2021/8822807
Species: Human
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:A549 CELL, DILUTION:NA
Liu Hui,Zhou Chuankun,Qi Dahu,Gao Yutong,Zhu Meipeng,Tao Tenghui,Sun Xuying,Xiao Jun,Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss.Frontiers in Cell and Developmental Biology,VOLUME=9,YEAR=2021, PAGES=544, URL=https://www.frontiersin.org/article/10.3389/fcell.2021.640867. DOI=10.3389/fcell.2021.640867,ISSN=2296-634X
Species: Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:BONE MARROW MACROPHAGES, DILUTION:NA
Huang T,Luo X,Wu B,Peng P,Dai Y,Hu G,Qiu H,Yuan X.Pyrotinib enhances the radiosensitivity of HER2‑overexpressing gastric and breast cancer cells.Oncol Rep.2020 Dec;44(6):2634-2644.doi:10.3892/or.2020.7820.Epub 2020 Oct 21.PMID:33125154;PMCID:PMC7640366.
Species: Human,Mouse
AR1160 usage in article: APP:CELL VIABILITY, SAMPLE:GC CELL AND BC CELL, DILUTION:NA
Guo J,Ren R,Yao X,Ye Y,Sun K,Lin J,Wang G, Guo F,Xiao J,Xu T.PKM2 suppresses osteogenesis and facilitates adipogenesis by regulating β-catenin signaling and mitochondrial fusion and fission.Aging (Albany NY).2020 Feb 25;12(4):3976-3992.doi:10. 18632/aging
Species: Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:BMSCS, DILUTION:NA
Zhang R,Zhang Q,Zou Z,Li Z,Jin M,An J,Li H,Ma J.Curcumin Supplementation Enhances Bone Marrow Mesenchymal Stem Cells to Promote the Anabolism of Articular Chondrocytes and Cartilage Repair.Cell Transplant.2021 Jan-Dec;30:963689721993776.doi:10.1177/096368
Species: Rat
AR1160 usage in article: APP:CELL VIABILITY, SAMPLE:BMSCS, DILUTION:NA
Shao N,Song L,Sun X.Exosomal circ_PIP5K1A regulates the progression of non-small cell lung cancer and cisplatin sensitivity by miR-101/ABCC1 axis.Mol Cell Biochem.2021 Feb 11.doi:10.1007/s11010-021-04083-8.Epub ahead of print.PMID:33570734.
Species: Human,Mouse
AR1160 usage in article: APP:CELL VIABILITY, SAMPLE:A549 CELL AND H1299 CELL, DILUTION:NA
Chu X,Yu T,Huang X,Xi Y,Ni B,Zhang R,You H.Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats.Aging (Albany NY).2020 Jul 5;12(13):12799-12811.doi:10.18632/aging.103222.Epub 2020
Species: Rat
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:PRIMARY CHONDROCYTES, DILUTION:NA
Shuai Bian,Yue Zhao,Fangyu Li,Shuyan Lu,Song Yang,Meichen Liu,Siming Wang,Daqing Zhao,Wei Zhang,Jiawen Wang,Knockdown of p62/sequestosome enhances ginsenoside Rh2-induced apoptosis in cervical cancer HeLa cells with no effect on autophagy,Bioscience,Biote
Species: Human
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:HELA CELL, DILUTION:NA
Wenpeng Cao,Zhirui Zeng,Zhiwei He et al.Hypoxia-Related Gene FUT11 Promoted Pancreatic Cancer Progression Via Maintaining The Stabilization of PDK1,27 January 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-152464/
Species: Human,Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:PANC-1 CELL AND ASPC-1 CELL, DILUTION:NA
Hua Y, Wang H, Wang H, Wu X, Yang L, Wang C, Li X, Jin Y, Li M, Wang L, Dong C, Yin F. Circular RNA Circ_0006282 Promotes Cell Proliferation and Metastasis in Gastric Cancer by Regulating MicroRNA-144-5p/Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase
Species: Human
AR1160 usage in article: APP:CELL PROLIFERATION ASSAY, SAMPLE:AGS CELL AND HGC-27 CELL, DILUTION:NA
Liu Y,Liu W,Zhang Z,Hu Y,Zhang X,Sun Y,Lei Q,Sun D,Liu T,Fan Y,Li H,Ding W,Fang J.Yishen capsule promotes podocyte autophagy through regulating SIRT1/NF-κB signaling pathway to improve diabetic nephropathy.Ren Fail.2021 Dec;43(1):128-140.doi:10.1080/08860
Species: Rat
AR1160 usage in article: APP:CELL PROLIFERATION ASSAY, SAMPLE:PODOCYTES, DILUTION:NA
Wenkai Yi,Tao Liu,Xinfeng Gao et al. 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice, 10 November 2020,PREPRINT (Version 1) available at Research Square [+https://doi.org/1
Species: Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:BMMS, DILUTION:NA
Xiaoling Zhang, Liangzhi Du, Ningbo Zhao et al. Effects of bone marrow mesenchymal stem cell-derived exosomes on bone formation on titanium, 05 November 2020, PREPRINT (Version 1) available at Research Square [+https:/ /doi. org/ 10. 2 1203/rs.3.rs-84302/
Species: Rabbit
AR1160 usage in article: APP:CELL PROLIFERATION ASSAY, SAMPLE:RABBIT BONE MARROW MESENCHYMAL STEM CELLS, DILUTION:NA
Yao X,Sun K,Yu S,Luo J,Guo J,Lin J,Wang G,Guo Z,Ye Y,Guo F. Chondrocyte ferroptosis contribute to the progression of osteoarthritis. J Orthop Translat. 2020 Dec 17;27:33-43. doi:10.1016/j.jot. 2020.09.006.PMID:33376672;PMCID:PMC7750492.
Species: Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:CHONDROCYTE, DILUTION:NA
Xubiao Ji,Zhiwen Song,Junsheng He,Shiwu Guo,Yuwei Chen,Honghai Wang,Jinlong Zhang,Xu Xu,Jinbo Liu,NIMA-related kinase 7 amplifies NLRP3 inflammasome pro-inflammatory signaling in microglia/mac rophages and mice models of spinal cord injury, Experimental C
Species: Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:BV-2 CELLS, DILUTION:NA
Wang W,Fang Q,Zhang Z,Wang D,Wu L,Wang Y. PPARα Ameliorates Doxorubicin-Induced Cardiotoxicity by Reducing Mitochondria-Dependent Apoptosis via Regulating MEOX1. Front Pharmacol.2020 Oct 8;11:528267. doi:10.3389/fphar.2020.528267.PMID:33132907;PMCID:PMC75
Species: Human,Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:H9C2 CELL,HEK293T CELL,A549 CELL,MDA-MB-231 CELL AND TC-1 CELL, DILUTION:NA
Qi YL,Li Y,Man XX,Sui HY,Zhao XL,Zhang PX,Qu XS,Zhang H,Wang BX,Li J,Qi SF,Jia LL,Luan HY,Zhang CB,Wang WQ.CXCL3 overexpression promotes the tumorigenic potential of uterine cervical cancer cells via the MAPK/ERK pathway.J Cell Physiol.2020 May;235(5):475
Species: Human,Mouse
AR1160 usage in article: APP:CELL PROLIFERATION ASSAY, SAMPLE:HELA CELL, DILUTION:NA
Wang Y,Liang Y,Li J,Hou R,Li J,Liang N,Xing J,Jiao J,Chang W,Li X,Zhang K.Expression and functional regulation of gap junction protein connexin 43 in dermal mesenchymal stem cells from psoriasis patients.Acta Histochem.2020 May;122(4):151550.doi:10.1016/j
Species: Human
AR1160 usage in article: APP:CELL PROLIFERATION, SAMPLE:DMSCS, DILUTION:NA
Yao X,Yu S,Jing X,Guo J,Sun K,Guo F,Ye Y.PTEN inhibitor VO-OHpic attenuates GC-associated endothelial progenitor cell dysfunction and osteonecrosis of the femoral head via activating Nrf2 signaling and inhibiting mitochondrial apoptosis pathway.Stem Cell
Species: Rat
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:EPCS, DILUTION:NA
Yang S,Wang H,Qin C,Sun H,Han Y.Up-regulation of CXCL8 expression is associated with a poor prognosis and enhances tumor cell malignant behaviors in liver cancer. Biosci Rep.2020 Aug 28;40(8):BSR20201169.doi: 10.1042/BSR20201169.PMID:32766720;PMCID:PMC744
Species: Human
AR1160 usage in article: APP:CELL PROLIFERATION ASSAY, SAMPLE:HEPG 2 CELL, DILUTION:NA
Liu J,Zhang Y,Liu W,Zhang Q,Xiao H,Song H,Luo B.MiR-BART1-5p targets core 2β-1,6-acetylglucosaminyltransferase GCNT3 to inhibit cell proliferation and migration in EBV-associated gastric cancer.Virology.2020 Feb;541:63-74.doi:10.1016/j.virol.2019.12.004.E
Species: AR1160;APP:CELL PROLIFERATION ASSAY;SAMPLE:GT38 CELL,GT39 CELL,SNU719 CELL,AGS CELL,HGC27 CELL,BGC823 CELL AND SGC7901 CELL; DILUTION:NA|AR1016;APP:IHC;SAMPLE:GASTRIC TISSUE;DILUTION:NA
Liao H,Li Y,Zhang X,Zhao X,Zheng D,Shen D,Li R. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.J Immunol Res.2020 Aug 27;2020:8263598.doi:10.1155/2020/ 8263598.PMID
Species: Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:PODOCYTES AND MACROPHAGES, DILUTION:NA
Li Y,Zheng D,Shen D,Zhang X,Zhao X,Liao H.Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M2 Polarization.J Immunol Res.2020
Species: Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:MACROPHAGES, DILUTION:NA
Cheng J, Xu T, Xun C, Guo H, Cao R, Gao S, Sheng W. Carnosic acid protects against ferroptosis in PC12 cells exposed to erastin through activation of Nrf2 pathway. Life Sci. 2020 Dec 14;266:118905. doi: 10.1016/j.lfs.2020.118905. Epub ahead of print. PMID
Species: Mouse
AR1160 usage in article: APP:CELL VIABILITY, SAMPLE:PC12 CELLS, DILUTION:NA
Lin G, Wang S, Zhang X, Wang D. Circular RNA circPLK1 promotes breast cancer cell proliferation, migration and invasion by regulating miR-4500/IGF1 axis. Cancer Cell Int. 2020 Dec 9;20(1):593. doi: 10.1186/s12935-020-01694-x. PMID: 33298061; PMCID: PMC772
Species: Human, Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:BT549 CELL AND HCC38 CELL, DILUTION:NA
Fu Y,Zhang Y,Lei Z,Liu T,Cai T,Wang A,Du W,Zeng Y,Zhu J,Liu Z,Huang JA. Abnormally activated OPN/integrin αVβ3/FAK signalling is responsible for EGFR-TKI resistance in EGFR mutant non-small-cell lung cancer. J Hematol Oncol.2020 Dec 7;13(1):169.doi:10.118
Species: Human,Mouse
AR1160 usage in article: APP:CELL VIABILITY, SAMPLE:PC9 CELL,HCC827 CELL AND H1975 CELL, DILUTION:NA
Guo Y, Wu Y, Li N, Wang Z. Up-regulation of miRNA-151-3p enhanced the neuroprotective effect of dexmedetomidine against β-amyloid by targeting DAPK-1 and TP53. Exp Mol Pathol. 2020 Dec 1:104587. doi: 10.1016/ j.yexmp.2020.104587. Epub ahead of print. PMID
Species: Human, Rat
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:SH-SY5Y AND PC12 CELLS, DILUTION:NA
Yi X,Chen W,Li C,Chen X,Lin Q,Lin S,Wang D. Circular RNA circ_0004507 contributes to laryngeal cancer progression and cisplatin resistance by sponging miR-873 to upregulate multidrug resistance 1 and multidrug resistance protein 1. Head Neck.2020 Nov 28.d
Species: Human,Mouse
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:AMC-HN-8 CELL AND TU-177 CELL, DILUTION:NA
Wang J, Bian S, Wang S, Yang S, Zhang W, Zhao D, Liu M, Bai X. Ginsenoside Rh2 represses autophagy to promote cervical cancer cell apoptosis during starvation. Chin Med. 2020 Nov 12;15(1):118. doi:10.1186/ s13020-020-00396-w. PMID: 33292331; PMCID: PMC766
Species: Human
AR1160 usage in article: APP:CELL VIABILITY ASSAY, SAMPLE:HEK293T CELLS, HELA CELLS AND C-33A CELLS, DILUTION:NA
Liu Y, Ju Y, Liu J, Chen Y, Huo X, Liu L. Inhibition of proliferation and migration and induction of apoptosis in glioma cells by silencing TLR4 expression levels via RNA interference. Oncol Lett. 2021 Jan;21(1):13. doi: 10.3892/ol.2020.12274. Epub 2020 N
Species: Human
AR1160 usage in article: APP:CELL VIABILITY, SAMPLE:U‑87MG‑SH, U‑87MG‑NC AND U‑87MG CELLS, DILUTION:NA
Xiao Z, Liang J, Deng Q, Song C, Yang X, Liu Z, Shao Z, Zhang K, Wang X, Li Z. Pimozide augments bromocriptine lethality in prolactinoma cells and in a xenograft model via the STAT5/cyclin D1 and STAT5/Bcl‑xL signaling pathways. Int J Mol Med. 2020 Nov 5.
Species: Human, Rat
AR1160 usage in article: APP:CELL VIABILITY, SAMPLE:MMQ CELLS, DILUTION:NA
Xiao Z, Yang X, Zhang K, Liu Z, Shao Z, Song C, Wang X, Li Z. Estrogen receptor α/prolactin receptor bilateral crosstalk promotes bromocriptine resistance in prolactinomas. Int J Med Sci. 2020 Oct 23;17(18):3174-3189. doi: 10.7150/ijms.51176. PMID: 331734
Species: Human
AR1160 usage in article: APP:CELL VIABILITY ASSAY, DILUTION:NA
Du W, Zhu J, Zeng Y, Liu T, Zhang Y, Cai T, Fu Y, Zhang W, Zhang R, Liu Z, Huang JA. KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway. Cell Death Differ. 2020 Nov 2.
Species: Human
AR1160 usage in article: APP:CELL VIABILITY, SAMPLE:HCC827 and H1299 CELLS, DILUTION:NA
FGFR2 Promotes Gastric Cancer Progression by Inhibiting the Expression of Thrombospondin4 via PI3K-Akt-Mtor Pathway
CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer
Expression of cold-inducible RNA-binding protein (CIRP) in renal cell carcinoma and the effect of CIRP downregulation cell proliferation and chemosensitivity to gemcitabine
Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA-7 in human hepatocellular carcinoma cells
miR-34a mediates oxaliplatin resistance of colorectal cancer cells by inhibiting macroautophagy via transforming growth factor-?/Smad4 pathway
MicroRNA-205 targets SMAD4 in non-small cell lung cancer and promotes lung cancer cell growth in vitro and in vivo
CLASP2 is involved in the EMT and early progression after transurethral resection of the bladder tumor
CD73/NT5E is a target of miR-30a-5p and plays an important role in the pathogenesis of non-small cell lung cancer
Three-dimensional Printed Scaffolds with Gelatin and Platelets Enhance In vitro Preosteoblast Growth Behavior and the Sustained-release Effect of Growth Factors
Downregulation of ZEB2-AS1 decreased tumor growth and metastasis in hepatocellular carcinoma
THBS2 is a Potential Prognostic Biomarker in Colorectal Cancer
IL-13/STAT6 signaling plays a critical role in the epithelial-mesenchymal transition of colorectal cancer cells
Effects of cisplatin on the LSD1-mediated invasion and metastasis of prostate cancer cells
Actein Inhibits Cell Proliferation and Migration in Human Osteosarcoma
Upregulated ATF6 contributes to chronic intermittent hypoxia-afforded protection against myocardial ischemia/reperfusion injury
Downregulation of HIF-1? inhibits the proliferation and invasion of non-small cell lung cancer NCI-H157 cells
SENP?1 enhances hypoxia?induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia?inducible factor?1?
The Mycobacterium bovis BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice
Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro
Downregulation of caveolin-1 upregulates the expression of growth factors and regulators in co-culture of fibroblasts with cancer cells
Demethylation of miR-10b plays a suppressive role in ccRCC cells
Nanog down-regulates the Wnt signaling pathway via ?-catenin phosphorylation during epidermal stem cell proliferation and differentiation
Single peptide ligand-functionalized uniform hollow mesoporous silica nanoparticles achieving dual-targeting drug delivery to tumor cells and angiogenic blood vessel cells
SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT
Overexpression of Wip1 Is Associated with Biologic Behavior in Human Clear Cell Renal Cell Carcinoma
Clinical implications of AGBL2 expression and its inhibitor latexin in breast cancer
Jia L, Li F, Shao M, Zhang W, Zhang C, Zhao X, Luan H, Qi Y, Zhang P, Liang L, Jia X, Zhang K, Lu Y, Yang Z, Zhu X, Zhang Q, Du J, Wang W Oncol Lett. 2018
Wang M, He SF, Liu LL, Sun XX, Yang F, Ge Q, Wong WK, Meng JY. Oncol Rep. 2017 Oct;38(4):1941-1948. doi: 10.3892/or.2017.5888. Epub 2017 Aug 8. Potential role of ZEB1 as a DNA repair regulator in colorectal cancer cells revealed by cancer-associat...
Sun Y, Ma GJ, Hu XJ, Yin XY, Peng YH. Oncol Lett. 2017 Dec;14(6):6511-6518. doi: 10.3892/ol.2017.7102. Epub 2017 Sep 28. Clinical significance of LMO1 in gastric cancer tissue and its association with apoptosis of cancer cells
Li Q, Li K, Yang T, Zhang S, Zhou Y, Li Z, Xiong J, Zhou F, Zhou X, Liu L, Meng R, Wu G. Sci Rep. 2017 Nov 23;7(1):16134. doi: 10.1038/s41598-017-16012-1. Association of protein kinase CK2 inhibition with cellular radiosensitivity of non-small cel...
Liu D, Zhang XX, Li MC, Cao CH, Wan DY, Xi BX, Tan JH, Wang J, Yang ZY, Feng XX, Ye F, Chen G, Wu P, Xi L, Wang H, Zhou JF, Feng ZH, Ma D, Gao QL. Nat Commun. 2018 Apr 30;9(1):1739. doi: 10.1038/s41467-018-03590-5. C/EBPβ enhances platinum resista...
Shi J, Lv Z, Nie M, Lu W, Liu C, Tian Y, Li L, Zhang G, Ren R, Zhang Z, Kang H. J Mol Histol. 2018 Jun;49(3):303-316. doi: 10.1007/s10735-018-9769-0. Epub 2018 Apr 9. Human nail stem cells are retained but hypofunctional during aging
Xu Y, Hou R, Lu Q, Zhang Y, Chen L, Zheng Y, Hu B. Am J Cancer Res. 2017 Dec 1;7(12):2545-2553. eCollection 2017. MiR-491-5p negatively regulates cell proliferation and motility by targeting PDGFRA in prostate cancer
Fang X, Han Q, Li S, Zhao Y, Luo A. Am J Transl Res. 2017 Sep 15;9(9):4288-4299. eCollection 2017. Chikusetsu saponin IVa attenuates isoflurane-induced neurotoxicity and cognitive deficits via SIRT1/ERK1/2 in developmental rats
Liu S, Li X, Wu Y, Duan R, Zhang J, Du F, Zhang Q, Li Y, Li N. PLoS One. 2017 Dec 14;12(12):e0189722. doi: 10.1371/journal.pone.0189722. eCollection 2017. Effects of vaspin on pancreatic β cell secretion via PI3K/Akt and NF-κB signaling pathways
Yan J, Wang J, Huang H, Huang Y, Mi T, Zhang C, Zhang L. Am J Transl Res. 2017 Oct 15;9(10):4492-4501. eCollection 2017. Fibroblast growth factor 21 delayed endothelial replicative senescence and protected cells from H2O2-induced premature senesce...
Lai J, Chen F, Chen J, Ruan G, He M, Chen C, Tang J, Wang DW. Sci Rep. 2017 Mar 14;7:44473. doi: 10.1038/srep44473. Overexpression of decorin promoted angiogenesis in diabetic cardiomyopathy via IGF1R-AKT-VEGF signaling
Yang Y, Hou J, Shao M, Zhang W, Qi Y, E S, Wang S, Sui H, Meng D, Wang B, Wang M, Han Y, Cao Y, Huang X, Li Y, Zhang P, Wang W. Oncol Lett. 2017 Dec;14(6):7977-7985. doi: 10.3892/ol.2017.7236. Epub 2017 Oct 20. CXCL5 as an autocrine or paracrine c...
Wu YX, Sun Y, Ye YP, Zhang P, Guo JC, Huang JM, Jing XZ, Xiang W, Yu SY, Guo FJ. Mol Med Rep. 2017 Dec;16(6):8200-8208. doi: 10.3892/mmr.2017.7648. Epub 2017 Sep 28. Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenes...
Guo J, Li W, Wu Y, Jing X, Huang J, Zhang J, Xiang W, Ren R, Lv Z, Xiao J, Guo F. Front Pharmacol. 2017 Oct 4;8:693. doi: 10.3389/fphar.2017.00693. eCollection 2017. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis ...
Xu X, Cao L, Zhang Y, Yin Y, Hu X, Cui Y. Oncol Lett. 2018 Jan;15(1):257-263. doi: 10.3892/ol.2017.7329. Epub 2017 Nov 2. Network analysis of DEGs and verification experiments reveal the notable roles of PTTG1 and MMP9 in lung cancer
Xiang W, Zhang J, Wang R, Wang L, Wang S, Wu Y, Dong Y, Guo F, Xu T. Mol Med Rep. 2018 Apr;17(4):4999-5006. doi: 10.3892/mmr.2018.8486. Epub 2018 Jan 25. Role of IFT88 in icariin-regulated maintenance of the chondrocyte phenotype
Zhu Y, Lu H, Zhang D, Li M, Sun X, Wan L, Yu D, Tian Y, Jin H, Lin A, Gao F, Lai M. Clin Epigenetics. 2018 Mar 2;10:30. doi: 10.1186/s13148-018-0458-3. eCollection 2018. Integrated analyses of multi-omics reveal global patterns of methylation and ...
Huang, X., Pan, Q., Mao, Z., Zhang, R., Ma, X., Xi, Y., & You, H. (2017). Sinapic Acid Inhibits the IL-1β-Induced Inflammation via MAPK Downregulation in Rat Chondrocytes. Inflammation, 1-7. Advance online publication. doi: 10.1007/s10753-017-0712-4
Lu, W., Zhang, H., Niu, Y., Wu, Y., Sun, W., Li, H.,…, & Wu, Y. (2017). Long non-coding RNA linc00673 regulated non-small cell lung cancer proliferation, migration, invasion and epithelial mesenchymal transition by sponging miR-150-5p. Molecular C...
Lan, T., Yan, X., Li, Z., Xu, X., Mao, Q., Ma, W.,…, & Yuan, Y. (2017). Long non-coding RNA PVT1 serves as a competing endogenous RNA for miR-186-5p to promote the tumorigenesis and metastasis of hepatocellular carcinoma. Tumor Biology, 39(6), 1-1...
Liu, L., Li, H., Cui, Y., Li, R., Meng, F., Ye, Z., & Zhang, X. (2017). Calcium Channel Opening Rather than the Release of ATP Causes the Apoptosis of Osteoblasts Induced by Overloaded Mechanical Stimulation. Cellular Physiology and Biochemistry, ...
Li R, Wu X, Wei H, Tian S. Oncol Lett. 2013 Mar;5(3):877-883. Epub 2013 Jan 2. Characterization Of Side Population Cells Isolated From The Gastric Cancer Cell Line Sgc-7901.
Ban Jb, Fan Xw, Huang Q, Li Bf, Chen C, Zhang Hc, Xu Sq. Plos One. 2014 May 16;9(5):E97607. Doi: 10.1371/Journal.Pone.0097607. Ecollection 2014. Mono-(2-Ethylhexyl) Phthalate Induces Injury In Human Umbilical Vein Endothelial Cells.
Ma Yj, He M, Han Ja, Yang L, Ji Xy. Scand J Immunol. 2013 Oct;78(4):387-93. Doi: 10.1111/Sji.12097. A Clinical Study Of Hbsag-Activated Dendritic Cells And Cytokine-Induced Killer Cells During The Treatment For Chronic Hepatitis B.
Cheng C, Wan F, Liu L, Zeng F, Xing S, Wu X, Chen X, Zhu Z. Plos One. 2014 May 16;9(5):E97406. Doi: 10.1371/Journal.Pone.0097406. Ecollection 2014. Overexpression Of Satb1 Is Associated With Biologic Behavior In Human Renal Cell Carcinoma.
Li Q, Wang Z. Arch Med Res. 2013 Oct;44(7):504-13. Doi: 10.1016/J.Arcmed.2013.09.009. Epub 2013 Oct 10. Influence Of Mesenchymal Stem Cells With Endothelial Progenitor Cells In Co-Culture On Osteogenesis And Angiogenesis: An In Vitro Study.
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2 Customer Q&As for Cell Counting Kit-8 (CCK-8)
Question
Does AR1160 contain the thimerosal or the sodium azide (or other toxic substances)? If so, what is the concentration?
Verified customer
Asked: 2019-05-23
Answer
The Cell Counting Kit-8 (CCK-8) (AR1160) does not contain thimerosal, sodium azide, or other toxic substances.
Boster Scientific Support
Answered: 2019-05-23
Question
Does AR1160 contain recombinant protein produced with baculovirus?
Verified customer
Asked: 2019-05-23
Answer
The Cell Counting Kit-8 (CCK-8) (AR1160) doesn't contain recombinant protein produced with baculovirus.
Boster Scientific Support
Answered: 2019-05-29
