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SKU AR1160
Product nameCell Counting Kit-8
Pack size 5 mL (for 500 assays, 10μL per well)
Publications: 90
Datasheet
Protocols

Product Overview

Form Liquid
Size 5 mL (for 500 assays, 10μL per well)
Concentration 6mg/ml
Buffer 9% NaCl
Storage Upon receipt store at -4°C, protect from light.
Note: Repeated thawing and freezing causes an increase in the background, which interferes with the assay.
Stability When stored as directed, product should be stable for one year. Store it at -20˚C for longer storage
Sensitivity CCK-8 is the highest sensitive dye for the cell based assay.
Equivalent Dojindo Molecular Technologies (Product No. CK04);
Millipore Sigma (Product No. 96992)

Introduction

Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt. WST-8 [2-(2- methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H- tetrazolium, monosodium salt]* produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. Cell Counting Kit-8 is a one-bottle solution; no premixing of components is required. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The cell proliferation assay using CCK-8 correlates well with the [(3)H] thymidine incorporation assay. And the CCK-8 assay can also be substituted for the [(3)H]thymidine incorporation assay. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

Advantages

1. One bottle, ready to use solution.

2. No organic solvents or isotopes required

3. No harvesting, no washing and no solubilization steps.

4. No Toxicity to Cell

5. More sensitive than MTT, XTT, MTS or WST-1

Precautions

1. Run pilot test to determine the optimal cell number and incubation time.

2. Since the CCK-8 assay is based on the dehydrogenase activity detection in viable cells, conditions or chemicals that affect dehydrogenase activity in viable cells may cause discrepancy between the actual viable cell number and the cell number determined using the CCK-8 assay.

3. WST-8 may react with reducing agents to generate WST-8 formazan. Please check the background O.D. if reducing agents are used in cytotoxicity assays or cell proliferation assays.

4. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.

5. Phenol red containing culture media can be used with this kit for cell viability assays.

6. Membrane filtration is recommended for the sterilization of the CCK-8 solution, if necessary.

7.The incubation time varies by the type and number of cells in a well. Generally, leukocytes give weak coloration, thus a long incubation time (up to 4 hours) or a large number of cells (~105 cells/well) may be necessary.

8. Since the cytotoxicity of this kit is very low, further color development is possible after reading the absorbance.

9.Neutral red or crystal violet can be used after the CCK-8 assay.

10.Measure the reference wavelength at 600 nm or higher if there is a high turbidity in the cell suspension.

Additional Materials Required

10μL and 100-200μL Multichannel Pipettes

Plate reader (450 nm filter)

96-well plate

CO2 incubator

General Protocol

Cell Number Determination

1. Inoculate cell suspension (100μL/well) in a 96-well plate. Pre-incubate the plate in a humidified incubator (e.g., at 37 ˚C, 5% CO2).

2. Add 10μL of the CCK-8 solution to each well of the plate. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.

3. Incubate the plate for 1-4 hours in the incubator.

4.Measure the absorbance at 450 nm using a microplate reader. To measure the absorbance later, add 10μL of 1% w/v SDS or 0.1M HCl to each well, cover the plate and store it with protection from light at room temperature. No absorbance change should be observed for 24 hours.

Cell proliferation and Cytotoxicity Assay

1. Dispense 100μL of cell suspension (5000 cells/well) in a 96- well plate. Pre-incubate the plate for 24 hours in a humidified incubator (e.g., at 37 ˚C, 5% CO2).

2. Add 10μL of various concentrations of substances to be tested to the plate.

3. Incubate the plate for an appropriate length of time (e.g., 6, 12, 24 or 48 hours) in the incubator.

4. Add 10μL of CCK-8 solution to each well of the plate. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.

5. Incubate the plate for 1-4 hours in the incubator.

6. Measure the absorbance at 450 nm using a microplate reader. To measure the absorbance later, add 10μL of 1% w/v SDS or 0.1M HCl to each well, cover the plate and store it with protection from light at room temperature. No absorbance change should be observed for 24 hours.

Frequently Asked Questions

1. How many cells should there be in a well?

For adhesive cells, at least 1000 cells are necessary per well (100μL medium). For leukocytes, at least 2500 cells are necessary per well (100μL medium) because of low sensitivity. The recommended maximum number of cells per well for the 96-well plate is 25000. If a 24 well or 6 well plate is used for this assay, please calculate the number of cells per well accordingly, and adjust the volume of the CCK-8 solution in a well to 10% of the total volume.

2. Does CCK-8 stain viable cells?

No. Since WST-8 and its formazan dye are highly water-soluble, CCK-8 cannot be utilized for cell staining purpose.

3. Does phenol red affect the assay?

No. The absorption value of phenol red in a culture medium can be removed by subtracting the absorption value of a blank solution from the absorption value of each well. Therefore, a medium containing phenol red is usable for the CCK-8 assay.

4. Is CCK-8 toxic to cells?

Since the toxicity of CCK-8 is so low, the same cells can be used for other cell proliferation assays such as the crystal violet assay, neutral red assay or DNA fluorometric assay after the CCK-8 assay is completed.

5. I do not have a 450 nm filter. What other filters can I use?

You can use filters with the absorbance between 430 and 490 nm, even though 450 nm filter gives the best sensitivity.

Cell Counting Kit-8 (CCK-8) Images

Click the images to enlarge.

Cell Counting Kit-8 (CCK-8)
Figure 1. Absorption spectrum of WST-8 formazan
Figure 1 shows the absorption spectrum of WST-8 formazan. Since the absorbance at 460 nm is proportional to the number of viable cells in the medium, the viable cell number can be determined using the absorbance of a previously prepared calibration curve.
Cell Counting Kit-8 (CCK-8)
Figure 2. Cell proliferation assay using CCK-8
Culture medium: MEM, 10% FBS
Incubation: 37 ˚C, 5% CO2, 2 hours
Deteactin: 450nm
Cell Counting Kit-8 (CCK-8)
Figure 3. Toxicological test of chemicals using CCK-8
Cell line: hela
Medium: DMEM, 10% FBS Chemicals: 200 μM Cisplatin (DDP)
Incubation: 37°C, 5% CO2, 2 hours
Cell Counting Kit-8 (CCK-8)
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Publications

CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer
PubMed ID29970127
Expression of cold-inducible RNA-binding protein (CIRP) in renal cell carcinoma and the effect of CIRP downregulation cell proliferation and chemosensitivity to gemcitabine
PubMed ID29849797
C/EBP? enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation
PubMed ID29712898
Human nail stem cells are retained but hypofunctional during aging
PubMed ID29633149
IL-8 is upregulated in cervical cancer tissues and is associated with the proliferation and migration of HeLa cervical cancer cells
PubMed ID29399185
Role of IFT88 in icariin-regulated maintenance of the chondrocyte phenotype
PubMed ID29393439
Network analysis of DEGs and verification experiments reveal the notable roles of PTTG1 and MMP9 in lung cancer
PubMed ID29387220
CXCL5 as an autocrine or paracrine cytokine is associated with proliferation and migration of hepatoblastoma HepG2 cells
PubMed ID29344240
Clinical significance of LMO1 in gastric cancer tissue and its association with apoptosis of cancer cells
PubMed ID29344115
MiR-491-5p negatively regulates cell proliferation and motility by targeting PDGFRA in prostate cancer
PubMed ID29312807
Effects of vaspin on pancreatic ? cell secretion via PI3K/Akt and NF-?B signaling pathways
PubMed ID29240812
Association of protein kinase CK2 inhibition with cellular radiosensitivity of non-small cell lung cancer
PubMed ID29170453
Fibroblast growth factor 21 delayed endothelial replicative senescence and protected cells from H2O2-induced premature senescence through SIRT1
PubMed ID29118911
Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR
PubMed ID29046637
Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-?
PubMed ID28983607
Chikusetsu saponin IVa attenuates isoflurane-induced neurotoxicity and cognitive deficits via SIRT1/ERK1/2 in developmental rats
PubMed ID28979702
Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA-7 in human hepatocellular carcinoma cells
PubMed ID28927113
Potential role of ZEB1 as a DNA repair regulator in colorectal cancer cells revealed by cancer-associated promoter profiling
PubMed ID28791382
Long non-coding RNA linc00673 regulated non-small cell lung cancer proliferation, migration, invasion and epithelial mesenchymal transition by sponging miR-150-5p
PubMed ID28697764
miR-34a mediates oxaliplatin resistance of colorectal cancer cells by inhibiting macroautophagy via transforming growth factor-?/Smad4 pathway
PubMed ID28348487
Overexpression of decorin promoted angiogenesis in diabetic cardiomyopathy via IGF1R-AKT-VEGF signaling
PubMed ID28290552
MicroRNA-205 targets SMAD4 in non-small cell lung cancer and promotes lung cancer cell growth in vitro and in vivo
PubMed ID28199217
CLASP2 is involved in the EMT and early progression after transurethral resection of the bladder tumor
PubMed ID28166762
CD73/NT5E is a target of miR-30a-5p and plays an important role in the pathogenesis of non-small cell lung cancer
PubMed ID28158983
Three-dimensional Printed Scaffolds with Gelatin and Platelets Enhance In vitro Preosteoblast Growth Behavior and the Sustained-release Effect of Growth Factors
PubMed ID27779164
Downregulation of ZEB2-AS1 decreased tumor growth and metastasis in hepatocellular carcinoma
PubMed ID27748842
THBS2 is a Potential Prognostic Biomarker in Colorectal Cancer
PubMed ID27632935
IL-13/STAT6 signaling plays a critical role in the epithelial-mesenchymal transition of colorectal cancer cells
PubMed ID27533463
Effects of cisplatin on the LSD1-mediated invasion and metastasis of prostate cancer cells
PubMed ID27484796
Actein Inhibits Cell Proliferation and Migration in Human Osteosarcoma
PubMed ID27173526
Upregulated ATF6 contributes to chronic intermittent hypoxia-afforded protection against myocardial ischemia/reperfusion injury
PubMed ID27035093
Downregulation of HIF-1? inhibits the proliferation and invasion of non-small cell lung cancer NCI-H157 cells
PubMed ID26998070
SENP?1 enhances hypoxia?induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia?inducible factor?1?
PubMed ID26935971
The Mycobacterium bovis BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice
PubMed ID26922320
Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro
PubMed ID26648136
Downregulation of caveolin-1 upregulates the expression of growth factors and regulators in co-culture of fibroblasts with cancer cells
PubMed ID26647977
Demethylation of miR-10b plays a suppressive role in ccRCC cells
PubMed ID26617769
Nanog down-regulates the Wnt signaling pathway via ?-catenin phosphorylation during epidermal stem cell proliferation and differentiation
PubMed ID25973172
Single peptide ligand-functionalized uniform hollow mesoporous silica nanoparticles achieving dual-targeting drug delivery to tumor cells and angiogenic blood vessel cells
PubMed ID25834425
SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT
PubMed ID25706386
Overexpression of Wip1 Is Associated with Biologic Behavior in Human Clear Cell Renal Cell Carcinoma
PubMed ID25334029
Hes-1 SUMOylation by protein inhibitor of activated STAT1 enhances the suppressing effect of Hes-1 on GADD45? expression to increase cell survival
PubMed ID24894488
Clinical implications of AGBL2 expression and its inhibitor latexin in breast cancer
PubMed ID24884516
Mono-(2-Ethylhexyl) Phthalate Induces Injury in Human Umbilical Vein Endothelial Cells
PubMed ID24836450
Overexpression of SATB1 Is Associated with Biologic Behavior in Human Renal Cell Carcinoma
PubMed ID24835085
Characterization of side population cells isolated from the gastric cancer cell line SGC-7901
PubMed ID23426065
miR-21, miR-17 and miR-19a induced by phosphatase of regenerating liver-3 promote the proliferation and metastasis of colon cancer
PubMed ID22677902
The Effect of Temozolomide/Poly(lactide-co-glycolide) (PLGA)/Nano-Hydroxyapatite Microspheres on Glioma U87 Cells Behavior
PubMed ID22312307
Jia L, Li F, Shao M, Zhang W, Zhang C, Zhao X, Luan H, Qi Y, Zhang P, Liang L, Jia X, Zhang K, Lu Y, Yang Z, Zhu X, Zhang Q, Du J, Wang W Oncol Lett. 2018
PubMed ID29399185
Wang M, He SF, Liu LL, Sun XX, Yang F, Ge Q, Wong WK, Meng JY. Oncol Rep. 2017 Oct;38(4):1941-1948. doi: 10.3892/or.2017.5888. Epub 2017 Aug 8. Potential role of ZEB1 as a DNA repair regulator in colorectal cancer cells revealed by cancer-associated promoter profiling
PubMed ID28791382
Sun Y, Ma GJ, Hu XJ, Yin XY, Peng YH. Oncol Lett. 2017 Dec;14(6):6511-6518. doi: 10.3892/ol.2017.7102. Epub 2017 Sep 28. Clinical significance of LMO1 in gastric cancer tissue and its association with apoptosis of cancer cells
PubMed ID29344115
Li Q, Li K, Yang T, Zhang S, Zhou Y, Li Z, Xiong J, Zhou F, Zhou X, Liu L, Meng R, Wu G. Sci Rep. 2017 Nov 23;7(1):16134. doi: 10.1038/s41598-017-16012-1. Association of protein kinase CK2 inhibition with cellular radiosensitivity of non-small cell lung cancer
PubMed ID29170453
Liu D, Zhang XX, Li MC, Cao CH, Wan DY, Xi BX, Tan JH, Wang J, Yang ZY, Feng XX, Ye F, Chen G, Wu P, Xi L, Wang H, Zhou JF, Feng ZH, Ma D, Gao QL. Nat Commun. 2018 Apr 30;9(1):1739. doi: 10.1038/s41467-018-03590-5. C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation
PubMed ID29712898
Shi J, Lv Z, Nie M, Lu W, Liu C, Tian Y, Li L, Zhang G, Ren R, Zhang Z, Kang H. J Mol Histol. 2018 Jun;49(3):303-316. doi: 10.1007/s10735-018-9769-0. Epub 2018 Apr 9. Human nail stem cells are retained but hypofunctional during aging
PubMed ID29633149
Xu Y, Hou R, Lu Q, Zhang Y, Chen L, Zheng Y, Hu B. Am J Cancer Res. 2017 Dec 1;7(12):2545-2553. eCollection 2017. MiR-491-5p negatively regulates cell proliferation and motility by targeting PDGFRA in prostate cancer
PubMed ID29312807
Fang X, Han Q, Li S, Zhao Y, Luo A. Am J Transl Res. 2017 Sep 15;9(9):4288-4299. eCollection 2017. Chikusetsu saponin IVa attenuates isoflurane-induced neurotoxicity and cognitive deficits via SIRT1/ERK1/2 in developmental rats
PubMed ID28979702
Liu S, Li X, Wu Y, Duan R, Zhang J, Du F, Zhang Q, Li Y, Li N. PLoS One. 2017 Dec 14;12(12):e0189722. doi: 10.1371/journal.pone.0189722. eCollection 2017. Effects of vaspin on pancreatic β cell secretion via PI3K/Akt and NF-κB signaling pathways
PubMed ID29240812
Yan J, Wang J, Huang H, Huang Y, Mi T, Zhang C, Zhang L. Am J Transl Res. 2017 Oct 15;9(10):4492-4501. eCollection 2017. Fibroblast growth factor 21 delayed endothelial replicative senescence and protected cells from H2O2-induced premature senescence through SIRT1
PubMed ID29118911
Lai J, Chen F, Chen J, Ruan G, He M, Chen C, Tang J, Wang DW. Sci Rep. 2017 Mar 14;7:44473. doi: 10.1038/srep44473. Overexpression of decorin promoted angiogenesis in diabetic cardiomyopathy via IGF1R-AKT-VEGF signaling
PubMed ID28290552
Yang Y, Hou J, Shao M, Zhang W, Qi Y, E S, Wang S, Sui H, Meng D, Wang B, Wang M, Han Y, Cao Y, Huang X, Li Y, Zhang P, Wang W. Oncol Lett. 2017 Dec;14(6):7977-7985. doi: 10.3892/ol.2017.7236. Epub 2017 Oct 20. CXCL5 as an autocrine or paracrine cytokine is associated with proliferation and migration of hepatoblastoma HepG2 cells
PubMed ID29344240
Wu YX, Sun Y, Ye YP, Zhang P, Guo JC, Huang JM, Jing XZ, Xiang W, Yu SY, Guo FJ. Mol Med Rep. 2017 Dec;16(6):8200-8208. doi: 10.3892/mmr.2017.7648. Epub 2017 Sep 28. Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-γ
PubMed ID28983607
Guo J, Li W, Wu Y, Jing X, Huang J, Zhang J, Xiang W, Ren R, Lv Z, Xiao J, Guo F. Front Pharmacol. 2017 Oct 4;8:693. doi: 10.3389/fphar.2017.00693. eCollection 2017. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR
PubMed ID29046637
Xu X, Cao L, Zhang Y, Yin Y, Hu X, Cui Y. Oncol Lett. 2018 Jan;15(1):257-263. doi: 10.3892/ol.2017.7329. Epub 2017 Nov 2. Network analysis of DEGs and verification experiments reveal the notable roles of PTTG1 and MMP9 in lung cancer
PubMed ID29387220
Xiang W, Zhang J, Wang R, Wang L, Wang S, Wu Y, Dong Y, Guo F, Xu T. Mol Med Rep. 2018 Apr;17(4):4999-5006. doi: 10.3892/mmr.2018.8486. Epub 2018 Jan 25. Role of IFT88 in icariin-regulated maintenance of the chondrocyte phenotype
PubMed ID29393439
Zhu Y, Lu H, Zhang D, Li M, Sun X, Wan L, Yu D, Tian Y, Jin H, Lin A, Gao F, Lai M. Clin Epigenetics. 2018 Mar 2;10:30. doi: 10.1186/s13148-018-0458-3. eCollection 2018. Integrated analyses of multi-omics reveal global patterns of methylation and hydroxymethylation and screen the tumor suppressive roles of HADHB in colorectal cancer
PubMed ID29507648
Huang, X., Pan, Q., Mao, Z., Zhang, R., Ma, X., Xi, Y., & You, H. (2017). Sinapic Acid Inhibits the IL-1β-Induced Inflammation via MAPK Downregulation in Rat Chondrocytes. Inflammation, 1-7. Advance online publication. doi: 10.1007/s10753-017-0712-4
PubMed ID29243030
Yang, Y., Hou, J., Shao, M., Zhang, W., Qi, Y., E, S.,..., & Wang, W. (2017). CXCL5 as an autocrine or paracrine cytokine is associated with proliferation and migration of hepatoblastoma HepG2 cells. Oncology Letters, 14(6), 7977-7985. doi: 10.3892/ol.2017.7236
PubMed ID-
Wang, Y., Wang, Q., & Song, J. (2017). Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA‑7 in human hepatocellular carcinoma cells. Oncology Letters, 14(3), 3566-3572. doi: 10.3892/ol.2017.6573
PubMed ID-
Lu, W., Zhang, H., Niu, Y., Wu, Y., Sun, W., Li, H.,…, & Wu, Y. (2017). Long non-coding RNA linc00673 regulated non-small cell lung cancer proliferation, migration, invasion and epithelial mesenchymal transition by sponging miR-150-5p. Molecular Cancer, 16(1), 118. doi: 10.1186/s12943-017-0685-9
PubMed ID28697764
Zheng, N., Li, M., Song, A., Song, L., & Sun, L. (2017). Downregulation of Expression of CHFR in RAJI Cells after Transfection with shRNA Promotes Growth and Inhibits Apoptosis. Cancer Cell Research, 4(13), 311-315. Retrieved from http://www.cancercellresearch.org/PDF/20171302.pdf
PubMed ID-
Feng, Y.H., Zhou, Z.X., Qiu, J., Chang, J.B., Wu, W.F. Li, L.,…, & Yang, Y.F. (2017). Immunotherapy of patients with chronic hepatitis B virus infection by HBsAg-activated dendritic cells combining with cytokine induced killer cells. International Journal of Clinical and Experimental Medicine, 10(6), 9589-9594. Retrieved from http://www.ijcem.com/files/ijcem0036105.pdf
PubMed ID-
Lan, T., Yan, X., Li, Z., Xu, X., Mao, Q., Ma, W.,…, & Yuan, Y. (2017). Long non-coding RNA PVT1 serves as a competing endogenous RNA for miR-186-5p to promote the tumorigenesis and metastasis of hepatocellular carcinoma. Tumor Biology, 39(6), 1-11. doi: 10.1177/1010428317705338
PubMed ID28656879
Liu, L., Li, H., Cui, Y., Li, R., Meng, F., Ye, Z., & Zhang, X. (2017). Calcium Channel Opening Rather than the Release of ATP Causes the Apoptosis of Osteoblasts Induced by Overloaded Mechanical Stimulation. Cellular Physiology and Biochemistry, 42(2), 441-454. Advance online publication. doi: 10.1159/000477592
PubMed ID28578345
Jianjie Zhu†, Yuanyuan Zeng†, Wei Li†, Hualong Qin†, Zhe Lei†, Dan Shen, Dongmei Gu, Jian-an HuangEmail author and Zeyi Liu Molecular Cancer201716:34 DOI: 10.1186/s12943-017-0591-1 CD73/NT5E is a target of miR-30a-5p and plays an important role in the pathogenesis of non-small cell lung cancer
PubMed ID-
VOL. 73 No. 6 November/December 2016 ISSN 2353-5288
PubMed ID-
Li R, Wu X, Wei H, Tian S. Oncol Lett. 2013 Mar;5(3):877-883. Epub 2013 Jan 2. Characterization Of Side Population Cells Isolated From The Gastric Cancer Cell Line Sgc-7901.
PubMed ID23426065
Ban Jb, Fan Xw, Huang Q, Li Bf, Chen C, Zhang Hc, Xu Sq. Plos One. 2014 May 16;9(5):E97607. Doi: 10.1371/Journal.Pone.0097607. Ecollection 2014. Mono-(2-Ethylhexyl) Phthalate Induces Injury In Human Umbilical Vein Endothelial Cells.
PubMed ID24836450
Ma Yj, He M, Han Ja, Yang L, Ji Xy. Scand J Immunol. 2013 Oct;78(4):387-93. Doi: 10.1111/Sji.12097. A Clinical Study Of Hbsag-Activated Dendritic Cells And Cytokine-Induced Killer Cells During The Treatment For Chronic Hepatitis B.
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Li Q, Wang Z. Arch Med Res. 2013 Oct;44(7):504-13. Doi: 10.1016/J.Arcmed.2013.09.009. Epub 2013 Oct 10. Influence Of Mesenchymal Stem Cells With Endothelial Progenitor Cells In Co-Culture On Osteogenesis And Angiogenesis: An In Vitro Study.
PubMed ID24120387
Zhang D, Tian A, Xue X, Wang M, Qiu B, Wu A. Int J Mol Sci. 2012;13(1):1109-25. Doi: 10.3390/Ijms13011109. Epub 2012 Jan 19. The Effect Of Temozolomide/Poly(Lactide-Co-Glycolide) (Plga)/Nano-Hydroxyapatite Microspheres On Glioma U87 Cells Behavior.
PubMed ID22312307
Zhang J, Xiao Z, Lai D, Sun J, He C, Chu Z, Ye H, Chen S, Wang J. Br J Cancer. 2012 Jul 10;107(2):352-9. Doi: 10.1038/Bjc.2012.251. Epub 2012 Jun 7. Mir-21, Mir-17 And Mir-19A Induced By Phosphatase Of Regenerating Liver-3 Promote The Proliferatio...
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Qin X, Yan L, Zhao X, Li C, Fu Y. Oncol Lett. 2012 Dec;4(6):1290-1296. Epub 2012 Sep 6. Microrna-21 Overexpression Contributes To Cell Proliferation By Targeting Pten In Endometrioid Endometrial Cancer.
PubMed ID23226804
Yu G, Li H, Wang X, Wu T, Zhu J, Huang S, Wan Y, Tang J. Mol Cell Biochem. 2013 Aug;380(1-2):239-47. Doi: 10.1007/S11010-013-1679-6. Epub 2013 May 12. Microrna-19A Targets Tissue Factor To Inhibit Colon Cancer Cells Migration And Invasion.
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Chiou Hy, Liu Sy, Lin Ch, Lee Eh. J Biomed Sci. 2014 Jun 4;21:53. Doi: 10.1186/1423-0127-21-53. Hes-1 Sumoylation By Protein Inhibitor Of Activated Stat1 Enhances The Suppressing Effect Of Hes-1 On Gadd45?? Expression To Increase Cell Survival.
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Ke S, Zhou F, Yang H, Wei Y, Gong J, Mei Z, Wu L, Yu H, Zhou Y. Int J Oncol. 2015 Mar;46(3):1051-8. Doi: 10.3892/Ijo.2014.2793. Epub 2014 Dec 10. Downregulation Of High Mobility Group Box 1 Modulates Telomere Homeostasis And Increases The Radiosen...
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Zhu H, Yun F, Shi X, Wang D. Mol Med Rep. 2015 Aug;12(2):3163-9. Doi: 10.3892/Mmr.2015.3684. Epub 2015 Apr 24. Vegf-C Inhibition Reverses Resistance Of Bladder Cancer Cells To Cisplatin Via Upregulating Maspin.
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Zhu H, Yun F, Shi X, Wang D. Int J Mol Med. 2015 Aug;36(2):595-601. Doi: 10.3892/Ijmm.2015.2250. Epub 2015 Jun 17. Inhibition Of Igfbp-2 Improves The Sensitivity Of Bladder Cancer Cells To Cisplatin Via Upregulating The Expression Of Maspin.
PubMed ID26080829
Liu Y, Zhao L, Ma W, Cao X, Chen H, Feng D, Liang J, Yin K, Jiang X. J Cancer. 2015 May 26;6(7):643-51. Doi: 10.7150/Jca.11913. Ecollection 2015. The Blockage Of Kca3.1 Channel Inhibited Proliferation, Migration And Promoted Apoptosis Of Human Hep...
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Yang Zt, Li Z, Wang Xg, Tan T, Yi F, Zhu H, Zhao Jp, Zhou Xf. Cell Physiol Biochem. 2015;35(6):2360-70. Doi: 10.1159/000374038. Epub 2015 Apr 15. Overexpression Of Long Non-Coding Rna Zxf2 Promotes Lung Adenocarcinoma Progression Through C-Myc Pat...
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Sun Kx, Xia Hw, Xia Rl. Int J Clin Exp Pathol. 2015 Jan 1;8(1):615-21. Ecollection 2015. Anticancer Effect Of Salidroside On Colon Cancer Through Inhibiting Jak2/Stat3 Signaling Pathway.
PubMed ID25755753