Cytoplasmic and Nuclear Protein Extraction Kit

Cytoplasmic and Nuclear Extraction Kit, Western Blotting Related Reagent Cited in 40 publication(s).

Product Info Summary

SKU AR0106
Pack size 1 kit
FormLiquid

Kit Components

DescriptionQuantityVolumeCatalog Number
Cytoplasmic Extraction Reagent A (CER A)130mLAR0106-A
Cytoplasmic Extraction Reagent B (CER B)11.5mLAR0106-B
Nuclear Extraction Reagent (NER)115mLAR0106-C

Overview

Product Name Cytoplasmic and Nuclear Protein Extraction Kit
KU/Catalog Number AR0106
Form Liquid
Pack Size 1 kit
Assays per kit 60 assays for cell pellet fractions having packed cell volumes of 50µL each
30 assays for 0.1g tissue
Storage Upon receipt store Cytoplasmic and Nuclear Protein Extraction Kit at 4°C. It is stable at 4°C for one year. Product is shipped at ambient temperature.
Compatibility with reagentsFully compatible with Broad Spectrum Protease Inhibitor Cocktail and Broad Spectrum Phosphatase Inhibitor Cocktail
Equivalent Thermofisher (Product No. 78833, 78835), Millipore Sigma (Product No. NXTRACT)
Description Boster’s Cytoplasmic and Nuclear Protein Extraction Kit provides a complete set of extraction reagents that enable the separation of nuclear protein and cytoplasmic fractions from cultured cells and fresh tissues.
Cite This Product Cytoplasmic and Nuclear Protein Extraction Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0106)

Assay Principle

This kit provides a complete set of extraction reagents that enable the separation of nuclear protein and cytoplasmic fractions from cultured cells and fresh tissues. The kit breaks cell membrane and release cytoplasmic proteins for cell burst under hypotonic condition. And then centrifuge for collection of the nucleoli. At last, extract nuclear proteins using Nuclear Extraction Reagent. Once desalted or diluted, the isolated soluble cytoplasmic proteins can be used to perform immunoassays and protein interaction experiments, such as EMSA, Co-IP and pull-down assays. Nuclear extracts are generally preferred to whole cell lysates for gene regulation studies. Cellular components present in whole cell lysates can adversely affect nuclear protein interactions and stability, and nuclear proteins are more concentrated in nuclear extracts than whole cell lysates.


Features of the Cytoplasmic and Nuclear Protein Extraction Kit:

Compatible—Extracted proteins can be directly apply for downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.

Fast—The optimized reagents and protocol allow non-denatured, active proteins to be purified in 90 minutes.

Convenient—simple instructions do not require ultracentrifugation over gradients.

Minimum cross-contamination—Cross-contamination of cytosolic proteins into the membrane fractions is usually about 10%.

Important Product Information

1. All steps of protein extraction should be operated on ice or at 4°C.

2. The kit is designed for fresh tissue samples only. It will not work efficiently for frozen tissue samples.

3. Use BCA protein Assay kit (Product No. AR0146) to quantify isolated proteins.

4. If more concentrated nuclear extracts are desired, the volume of NER used in the extractions can be decreased 2- to 4-fold without adverse effects on protein recovery or compartmentalization.

5. If large volumes of nuclear extract are required in subsequent applications or if problems occur with downstream assays, dialyze the nuclear extract to remove excess salts before use.

6. Include protease inhibitors to maintain extract integrity and function.

Additional Materials Required

• Protease inhibitor (Product No. AR1182) and phosphatase inhibitor (Product No. AR1183)

• 2mL microcentrifuge tubes

• Vortex mixer

• Microcentrifuge capable of spinning at 16,000 x g

• Tissue homogenizer

• Phosphate-buffered saline (PBS): 0.1M phosphate, 0.15M sodium chloride; pH 7.2

Procedure for Cytoplasmic and Nuclear Protein Extraction from Different Sample Types

Reagent Preparation:
Place Cytoplasmic Extraction Reagent A (CER A), Cytoplasmic Extraction Reagent B (CER B) and Nuclear Extraction Reagent (NER) on ice. For optimal results, include protease inhibitor and phosphatase inhibitor before use.

Protocol 1: Adherent Cells & Suspension Cells

Cell Culture Preparation

1. For adherent cells: scrape the cells off the surface of the plate with a cell scraper. Centrifuge harvested cells at 600 x g for 5 minutes. Carefully remove and discard the supernatant, and keep cell pellets for use.

2. For suspension cells: centrifuge harvested cells at 600 x g for 5 minutes. Carefully remove and discard the supernatant, and keep cell pellets for use.

3. Resuspend the cells in pre-cooling PBS.

4. Transfer the cells to a 2mL microcentrifuge tube. Centrifuge at 600 x g for 5 minutes. Carefully remove and discard the supernatant, and keep cell pellets for use.

5. Add CER A to the cell pellet according to the volumes indicated in Table 1.
Table 1. Reagent volumes for different packed cell volumes

Packed cell Volume (μL)CER A (μL)CER B (μL)NER (μL)
10100550
2020010100
5050025.5250
100100050500

The volume of 2×106 Hela cells is about 20μL.

6. Vortex the tube at maximum speed for 15 seconds to obtain a homogeneous cell suspension. Incubate on ice for 10-15 minutes.

7. Add CER B to the tube. Vortex the tube at maximum speed for 5 seconds. Incubate on ice for 1 minute. (If there is cytoplasmic protein in extracted nuclear protein, prolong the vortex time by 1-5 minutes.)

8. Vortex the tube at maximum speed for 5 seconds. Centrifuge the tube at 16000 × g for 5 minutes.

9. Immediately transfer the supernatant containing cytoplasmic proteins to a clean pre-chilled tube. Place this tube on ice until use or store aliquots at -80°C for future use.

10. Add NER to the insoluble cell debris containing nuclei produced in step 8.

11. Vortex at maximum speed for 5 seconds to obtain a homogeneous cell suspension (If not complete, prolong the vortex time). Place the cell debris on ice and continue vortexing for 15 seconds every 10 minutes, for a total of 40 minutes.

12. Centrifuge the tube at 16000 × g for 5 minutes.

13. Immediately transfer the supernatant containing nuclear proteins to a clean pre-chilled tube. Place this tube on ice until use or store aliquots at -80°C for future use.


Protocol 2: Tissue

Tissue Preparation:

1. Place the fresh tissue into pre-chilled PBS and rinse several times. Dry the tissue with filter paper. Mince the tissue into small pieces and weigh the tissue sample.

2. Place tissue in a tissue homogenizer. Add CER A to the tissue according to the volumes indicated in Table 2.
Table 2. Reagent volumes for different tissue amounts

Tissue Weight (mg)CER A (μL)CER B (μL)NER (μL)
2020010100
4040020200
8080040400
100100050500

3. Homogenize tissue on ice to obtain a homogeneous suspension. Transfer the homogeneous suspension to a 2mL microcentrifuge tube. Incubate on ice for 10 minutes.

4. Add CER B to the tube. Vortex the tube at maximum speed for 5 seconds. Incubate on ice for 1 minute.

5. Vortex the tube at maximum speed for 5 seconds. Centrifuge the tube at 16000 × g for 5 minutes.

6. Immediately transfer the supernatant containing cytoplasmic proteins to a clean pre-chilled tube. Place this tube on ice until use or store aliquots at -80°C for future use.

7. Add NER to the insoluble cell debris containing nuclei produced in step 5.

8. Vortex at maximum speed for 5 seconds to obtain a homogeneous cell suspension (If not complete, prolong the vortex time). Place the cell debris on ice and continue vortexing for 15 seconds every 10 minutes, for a total of 40 minutes.

9. Centrifuge the tube at 16000 × g for 5 minutes.

10. Immediately transfer the supernatant containing nuclear proteins to a clean pre-chilled tube. Place this tube on ice until use or store aliquots at -80°C for future use.

Note:
For some tissues, if the cytoplasmic or nuclear proteins fail to be extracted as expected, follow Protocol 3.

Protocol 3: Tissue

1. Place the fresh tissue into pre-chilled PBS and rinse several times. Dry the tissue with filter paper. Mince the tissue into small pieces and weigh the tissue sample.

2. Place tissue in a tissue homogenizer.

3. Mix CER A with CER B at a volume ratio of 20:1 to generate CER A and CER B Mixture. e.g. Add 10μL of CER B into 200μL of CER A.

4. Add CER A and CER B Mixture to the tissue at a ratio of 10:1 (v/w). e.g. Add 200μL of CER A and CER B Mixture to 20mg tissue.

5. Homogenize tissue on ice to obtain a homogeneous suspension. Transfer the tissue homogenate to a 2mL microcentrifuge tube. Incubate on ice for 15 minutes.

6. Centrifuge the tube at 1500 × g for 5 minutes at 4 ˚C.

7. Immediately transfer the supernatant containing cytoplasmic proteins to a clean pre-chilled tube. Place this tube on ice until use or store aliquots at -80°C for future use. (These are partial cytoplasmic proteins extracted from the tissue sample. Do not touch the pellet when aspirating the supernatant. There are still many cells in the pellet that have not been broken.)

8. Add CER A to the cell pellet according to the volumes indicated in Table 3.
Table 3. Reagent volumes for different packed cell volumes

Packed cell Volume (μL)CER A (μL)CER B (μL)NER (μL)
10100550
2020010100
5050025.5250
100100050500

The volume of 2×106 Hela cells is about 20μL.

9. Vortex the tube at maximum speed for 15 seconds to obtain a homogeneous cell suspension. Incubate on ice for 10-15 minutes.

10. Add CER B to the tube. Vortex the tube at maximum speed for 5 seconds. Incubate on ice for 1 minute.

11. Vortex the tube at maximum speed for 5 seconds. Centrifuge the tube at 16000 × g for 5 minutes.

12. Immediately transfer the supernatant containing cytoplasmic proteins to a clean pre-chilled tube. Place this tube on ice until use or store aliquots at -80°C for future use.

13. Add NER to the insoluble cell debris containing nuclei produced in step 8.

14. Vortex at maximum speed for 5 seconds to obtain a homogeneous cell suspension (If not complete, prolong the vortex time). Place the cell debris on ice and continue vortexing for 15 seconds every 10 minutes, for a total of 40 minutes.

15. Centrifuge the tube at 16000 × g for 5 minutes.

16. Immediately transfer the supernatant containing nuclear proteins to a clean pre-chilled tube. Place this tube on ice until use or store aliquots at -80°C for future use.

Note: Cytoplasmic proteins produced in Step 6 and Step 11 can be stored and used together.

Troubleshooting

ProblemPossible CauseSolution
Low cytoplasmic protein yieldCells were not lysed completely
Cell pellet was not dispersed
Tissues was not homogenized sufficiently
Increase amount of CER A and CER B Reagent
Vortex thoroughly
Homogenize sufficiently
Low nuclear protein yieldCell pellet was not dispersed
ncomplete nuclei isolation
Vortex thoroughly
Increase time of centrifugation
No or low protein activity detectedSamples were not kept cold
Presence of protease
Keep samples on ice between vortexing steps
Use a protease inhibitor cocktail
Proteins not compartmentalizedExtraction time for cytoplasmic protein is too long.
ncomplete removal of cytoplasmic extract
Cytoplasmic extract contains some nuclear precipitation while transferring the supernatant containing cytoplasmic proteins
Decrease extraction time for cytoplasmic protein.
Carefully remove all cytoplasmic extract before nuclear lysis
Carefully remove all cytoplasmic extract before nuclear lysis

Related Boster Products

AR1182 Broad Spectrum Protease Inhibitor Cocktail
AR1183 Broad Spectrum Phosphatase Inhibitor Cocktail
AR0146 BCA Protein Assay Kit
AR0131 SDS-PAGE Loading Buffer (2×)

Product Images

AR0106 has been cited in 40 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhao,Y.,Jia,W.,Ren,S.,Xiao,W.,Li,G.,Jin,L.,& Lin,Y.(2021). Difluoromethylornithine attenuates isoproterenol‑induced cardiac hypertrophy by regulating apoptosis, autophagy and the mitochondria‑associated membranes pathway.Experimental and Therapeutic Medicine,22,870.https://doi.org/10.3892/etm.2021.10302
Species: Rat
AR0106 usage in article: APP:WB, SAMPLE:VENTRICLE TISSUE, DILUTION:NA

Xu H,Wang Y,Luo Y.OTULIN is a new target of EA treatment in the alleviation of brain injury and glial cell activation via suppression of the NF-κB signalling pathway in acute ischaemic stroke rats.Mol Med.2021 Apr 9;27(1):37.doi:10.1186/s10020-021-00297-0.PMID:33836646;PMCID:PMC8035756.
Species: Rat
AR0106 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA

Jin XH, Zhang YF, Yuan YX, Han L, Zhang GP, Hu H. Isolation, characterization and transcriptome analysis of porcine deltacoronavirus strain HNZK-02 from Henan Province, China. Mol Immunol. 2021 Mar 16;134:86-99. doi: 10.1016/j.molimm.2021.03.006. Epub ahead of print. PMID: 33740580.
Species: Pig
AR0106 usage in article: APP:WB, SAMPLE:ST CELL, DILUTION:NA

Hongbei Xu,You Wang,Yong Luo et al. OTULIN is a New Target for EA Treatment in Alleviating Brain Injury and The Activation of Glial Cells By Depressing NF-κB Signalling Pathway in Acute Ischaemic Stroke Rats,28 January 2021,PREPRINT (Version 1) available
Species: Rat
AR0106 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA

Bai Y,Wang X,Cai M,Ma C,Xiang Y,Hu W,Zhou B,Zhao C,Dai X,Li X,Zhao H.Cinobufagin suppresses colorectal cancer growth via STAT3 pathway inhibition.Am J Cancer Res.2021 Jan 1;11(1):200-214.PMID:33520369;PMCID:PMC7840714.
Species: Human,Mouse
AR0106 usage in article: APP:WB, SAMPLE:CRC CELL, DILUTION:NA

Srinivasan G,Parida S,Pavithra S, Panigrahi M,Sahoo M,Singh TU,Madhu CL,Manickam K,Shyamkumar TS,Kumar D,Mishra SK.Leptin receptor stimulation in late pregnant mouse uterine tissue inhibits spontaneous contractions by increasing NO and cGMP.Cytokine.2021
Species: Mouse
AR0106 usage in article: APP:WB, SAMPLE:UTERINE TISSUE, DILUTION:NA

Wang J,Wang L,Chen S,Peng H,Xiao L,E Du,Liu Y,Lin D,Wang Y,Xu Y,Yang K.PKMYT1 is associated with prostate cancer malignancy and may serve as a therapeutic target.Gene.2020 Jun 20;744:144608.doi:10.1016/j. gene.2020.144608.Epub 2020 Mar 29.PMID:32234541.
Species: Human
AR0106 usage in article: APP:WB, SAMPLE:LNCAP CELL,C4-2 CELL,PC-3 CELL AND DU 145 CELL, DILUTION:NA

Zhu W,Yang F,Cai X,Zhang W,Zhang J,Cai M,Li X,Xiang J,Cai D.Role of glucocorticoid receptor phosphorylation-mediated synaptic plasticity in anxiogenic and depressive behaviors induced by monosodium glutamate. Naunyn Schmiedebergs Arch Pharmacol.2020 May 2
Species: Rat
AR0106 usage in article: APP:WB, SAMPLE:MPFC, HYPOTHALAMUS AND HIPPOCAMPUL TISSUES, DILUTION:NA

Liu Y, Yang M, Cui Y, Yao Y, Liao M, Yuan H, Gong G, Deng S, Zhao G. A novel prevascularized tissue-engineered chamber as a site for allogeneic and xenogeneic islet transplantation to establish a bioartificial pancreas. PLoS One. 2020 Dec 3;15(12):e023467
Species: Rat, Mouse
AR0106 usage in article: APP:WB, SAMPLE:ADIPOSE TISSUE, DILUTION:NA

Chen H, Teng Y, Liu Z, Geng F, Chen X, Jiang H, Yang J, Zheng M, Wang Z, Yang L. Molecular Mechanism of Platelet-Derived Growth Factor (PDGF)-BB-Mediated Protection Against MPP+ Toxicity in SH-SY5Y Cells. J Mol Neurosci. 2020 Nov 9. doi: 10.1007/s12031-02
Species: Human
AR0106 usage in article: APP:WB, SAMPLE:SH-SY5Y CELLS, DILUTION:NA

Xiao Z, Yang X, Zhang K, Liu Z, Shao Z, Song C, Wang X, Li Z. Estrogen receptor α/prolactin receptor bilateral crosstalk promotes bromocriptine resistance in prolactinomas. Int J Med Sci. 2020 Oct 23;17(18):3174-3189. doi: 10.7150/ijms.51176. PMID: 331734
Species: Human
AR0106 usage in article: APP:WB, SAMPLE:PROLACTINOMA, DILUTION:NA

The diabetes drug semaglutide reduces infarct size, inflammation, and apoptosis, and normalizes neurogenesis in a rat model of stroke Xiaoyan Yang 1, Peng Feng 2, Xiangjian Zhang 3, Dongfang Li 1, Ruifang Wang 1, Chenhui Ji 4, Guanglai Li 1, Christian H

Carnosine Prevents Type 2 Diabetes-Induced Osteoarthritis Through the ROS/NF-?B Pathway

Early Minocycline and Late FK506 Treatment Improves Survival and Alleviates Neuroinflammation, Neurodegeneration, and Behavioral Deficits in Prion-Infected Hamsters

Mycobacterium bovis Induces Endoplasmic Reticulum Stress Mediated-Apoptosis by Activating IRF3 in a Murine Macrophage Cell Line

Liver Fatty Acid Binding Protein Deficiency Provokes Oxidative Stress, Inflammation, and Apoptosis-Mediated Hepatotoxicity Induced by Pyrazinamide in Zebrafish Larvae

The ORF3 Protein of Genotype 1 Hepatitis E Virus Suppresses TLR3-induced NF-?B Signaling via TRADD and RIP1

Anti-inflammatory properties of lipoxin A4 protect against diabetes mellitus complicated by focal cerebral ischemia/reperfusion injury

Inhibition of Acute Lung Injury by TNFR-Fc through Regulation of an Inflammation-Oxidative Stress Pathway

Tongxinluo Protects against Hypertensive Kidney Injury in Spontaneously-Hypertensive Rats by Inhibiting Oxidative Stress and Activating Forkhead Box O1 Signaling

Trimetazidine prevents macrophage?mediated septic myocardial dysfunction via activation of the histone deacetylase sirtuin 1

Endogenous FOXP3 inhibits cell proliferation, migration and invasion in glioma cells

Open Reading Frame 3 of Genotype 1 Hepatitis E Virus Inhibits Nuclear Factor-?appa B Signaling Induced by Tumor Necrosis Factor-? in Human A549 Lung Epithelial Cells

Involvement of p38 in signal switching from autophagy to apoptosis via the PERK/eIF2?/ATF4 axis in selenite-treated NB4 cells

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke

The Zinc Finger Protein ZNF268 Is Overexpressed in Human Cervical Cancer and Contributes to Tumorigenesis via Enhancing NF-?B Signaling

The NALP3 inflammasome is involved in neurotoxic prion peptide-induced microglial activation

Knockdown of ZNF268, which Is Transcriptionally Downregulated by GATA-1, Promotes Proliferation of K562 Cells

Xu Y, Jiang Y, Gai W, Yu B. Exp Ther Med. 2017 Nov;14(5):5051-5056. doi: 10.3892/etm.2017.5164. Epub 2017 Sep 21. Protective role of tenuigenin on sepsis-induced acute kidney injury in mice

Lai M, Yao H, Shah SZA, Wu W, Wang D, Zhao Y, Wang L, Zhou X, Zhao D, Yang L. Front Aging Neurosci. 2018 Apr 18;10:116. doi: 10.3389/fnagi.2018.00116. eCollection 2018. The NLRP3-Caspase 1 Inflammasome Negatively Regulates Autophagy via TLR4-TRIF ...

Zhao X, Cui Q, Fu Q, Song X, Jia R, Yang Y, Zou Y, Li L, He C, Liang X, Yin L, Lin J, Ye G, Shu G, Zhao L, Shi F, Lv C, Yin Z. Sci Rep. 2017 Aug 18;7(1):8782. doi: 10.1038/s41598-017-09365-0. Antiviral properties of resveratrol against pseudorabie...

Jiang Y, Yu M, Hu X, Han L, Yang K, Ba H, Zhang Z, Yin B, Yang XP, Li Z, Wang J. Cell Death Differ. 2017 Apr;24(4):660-671. doi: 10.1038/cdd.2016.162. Epub 2017 Feb 10. STAT1 mediates transmembrane TNF-alpha-induced formation of death-inducing sig...

Song Z, Shah SZA, Yang W, Dong H, Yang L, Zhou X, Zhao D. Front Mol Neurosci. 2017 May 3;10:128. doi: 10.3389/fnmol.2017.00128. eCollection 2017. Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Ak...

Zhou C, Huang J, Li Q, Nie J, Xu X, Wang DW. Sci Rep. 2017 Jul 31;7(1):6926. doi: 10.1038/s41598-017-07512-1. Soluble Epoxide Hydrolase Inhibition Protected against Angiotensin II-induced Adventitial Remodeling

Wang G, Hu Z, Fu Q, Song X, Cui Q, Jia R, Zou Y, He C, Li L, Yin Z. Sci Rep. 2017 Mar 21;7:45006. doi: 10.1038/srep45006. Resveratrol mitigates lipopolysaccharide-mediated acute inflammation in rats by inhibiting the TLR4/NF-κBp65/MAPKs signaling ...

Xu H, Qin W, Hu X, Mu S, Zhu J, Lu W, Luo Y. J Neuroinflammation. 2018 Mar 15;15(1):83. doi: 10.1186/s12974-018-1117-5. Lentivirus-mediated overexpression of OTULIN ameliorates microglia activation and neuroinflammation by depressing the activatio...

Deng C, Cao J, Han J, Li J, Li Z, Shi N, He J. Comput Intell Neurosci. 2018 Feb 12;2018:3094504. doi: 10.1155/2018/3094504. eCollection 2018. Liraglutide Activates the Nrf2/HO-1 Antioxidant Pathway and Protects Brain Nerve Cells against Cerebral I...

Song, Z., Yang, W., Zhou, X., Yang, L., & Zhao, D. (2017). Lithium alleviates neurotoxic prion peptide-induced synaptic damage and neuronal death partially by the upregulation of nuclear target REST and the restoration of Wnt signaling. Neuropharm...

Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...

Jiang Q, Wang Y, Li T, Shi K, Li Z, Ma Y, Li F, Luo H, Yang Y, Xu C. Mol Biol Cell. 2011 Apr 15;22(8):1167-80. Doi: 10.1091/Mbc.E10-10-0860. Epub 2011 Feb 23. Heat Shock Protein 90-Mediated Inactivation Of Nuclear Factor-??b Switches Autophagy To ...

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4 Customer Q&As for Cytoplasmic and Nuclear Protein Extraction Kit

Question

Is AR0106 compatible with biorad assey?

Verified customer

Asked: 2021-02-26

Answer

Our lab hasn't used DC™ Protein Assay Kit II 5000112 from Biorad and we haven't validated if the Cytoplasmic And Nuclear Protein Extraction Kit (AR0106) is compatible with the kit. We suggest to run a pilot test and see if they are compatible.

Boster Scientific Support

Answered: 2021-02-28

Question

What is the composition of AR0106?

Verified customer

Asked: 2021-02-26

Answer

Unfiortunately, we can't disclose the composition of the Cytoplasmic And Nuclear Protein Extraction Kit (AR0106).

Boster Scientific Support

Answered: 2021-02-28

Question

What is the solution for problem with dissolving the pellet in the Nunclear Extraction Reagent even when increasing the vortex time and sufficient amount of the NER?

Verified customer

Asked: 2020-10-27

Answer

For the Cytoplasmic And Nuclear Protein Extraction Kit (AR0106), please continue to increase vortex time and strength.

Boster Scientific Support

Answered: 2020-10-28

Question

Can AR0106 be used with frozen cells or must they be freshly harvested?

Verified customer

Asked: 2019-07-15

Answer

The Cytoplasmic And Nuclear Protein Extraction Kit (AR0106) is designed for fresh tissue samples only. It will not work efficiently for frozen tissue samples.

Boster Scientific Support

Answered: 2019-07-15

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