DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining

DAPI Stain Solution, IHC Related Reagent, For nucleus restaining in Immunofluorescence experiments Cited in 10 publication(s).

Product Info Summary

SKU: AR1176
Size: From 10mL to 50mL

Product Overview

Product Name DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining
Material DAPI dihydrochloride (MW = 350.3)
Form Liquid
Size 10 mL (100 assays)/50 mL (500 assays)
Concentration 1μg/ml
Buffer 8 mM sodium phosphate, 2 mM potassium phosphate, 140 mM sodium chloride, 10 mM potassium chloride; pH 7.4.
Storage Upon receipt store at -20°C, protect from light.
Stability When stored as directed, product should be stable for one year.
Molecular formula C16H15N5 • 2 HCL
Excitation: DAPI 340nm
Emission: DAPI 488nm
Excitation: DAPI-DNA complexes 360nm
Emission: DAPI-DNA complexes 460nm
Equivalent Thermofisher (Product No. 62247); Thermofisher (Product No. 62248); Millipore Sigma (Product No. D9542)

Notes

Type of DAPIMolecular formulaMolecular weightCatalog Number
DAPI dihydrochlorideC16H15N5 • 2 HCL350.25AR1176-10
DAPI dilactateC16H15N5 • 2 C3H6O3457.48N/A

Introduction

DAPI (4',6-diamidino-2-phenylindole) is a fluorescent dye which can bind DNA strands robustly, the fluorescence being detected by fluorescence microscope. DAPI can dye both live and fixed cells as it can cross intact membrane, with higher efficiency in fixed cells. The molecular formula is C16H15N5•2HCl with 350.25 g/mol molecular weight, CAS Number 28718-90-3. DAPI could pass through the cell and nucleic membranes and bind the double-strand DNA in the nucleus, producing 20 times stronger fluorescence than itself. The efficiency detected by fluorescence microscope is very high (almost 100%), having no side effects for the live cells. The sensitivity for double stranded DNA DAPI staining is many times larger comparing to ethidium bromide (EB). DAPI staining is usually used in cell death detection, as it enters more effectively and generates stronger fluorescence in dead cells. After staining with DAPI, detect with fluorescence microscope or flow cytometry. Blue fluorescent cell would be seen under the microscope after staining. The largest excitation wavelength for DAPI is 340nm (ultraviolet), and the largest emission wavelength is 488nm (blue). When DAPI binds with double-strand DNA, the largest excitation wavelength is 360nm, while the largest emission wavelength becomes 460nm. DAPI's blue emission makes it suitable for combined assays where the fluorescence ranges of DAPI and other IHC-employed fluorescent molecules like green-fluorescent fluorescein and GFP, or red-fluorescent stains like Texas Red, are completely distinctive.

Properties

Enzymatic Activity DNase-, RNase-, Protease- free
Cell Permeability Intact membrane permeant
Sub-Cellular Localization: Nucleus
Biomolecule Reactivity Specific binding to AT-base pairs; intercalation into GC-base pairs;
Cite This Product DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1176)

Application

Fluorescent bioimaging; Immunofluorescence assays, flow cytometry; in situ hybridization; hystology; IHC; HCS; quantification of DNA content; labeling of DNA in cell cultures; cell death detection

Usage and Handling

    For DNA and chromosomes staining, In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
    DAPI is a known mutagen and should be handled with care.

Protocol

1. Fixed cells and tissues: wash appropriately to remove fixative. If necessary, immunofluorescent staining can be performed first, then perform the DAPI staining. If there is no other staining, perform DAPI staining directly.
Adherent cells or tissue slices: add a sufficient volume of DAPI stain solution to completely cover the sample.
Suspension cells: Add DAPI stain solution to suspend cell pellets at 3:1. Vortex to obtain an even suspension.

2. Incubate for 5-10 minutes at RT (room temperature).

3. Remove the stain solution.

4. Wash with TBST, PBS or physiological saline for 2-3 times, 3-5 minutes each.

5. Observe the sample under fluorescence microscope (excitation wavelength: 360nm, emission wavelength:460nm).


Notes:

1. Use Boster Antifade Mounting Medium (Product No. AR1109) to prevent rapid photobleaching of fluorescence within spectrum range.

2. Please wear the lab coat and disposable gloves to operate.

Chemical Information

Chemical Name 4′,6-Diamidino-2-phenylindole dihydrochloride
IUPAC Name 2- (4-Amidinophenyl)-1H-indole-6-carboxamidine dihidrochloride
Molecular Formula C16H15N5·2HCl; C16H17Cl2N5
Molecular Weight 350.25
CAS 28718-90-3
EC Number 249-186-7
Chemical Sources ChEMBL: CHEMBL48217; CHEMBL531243; SCHEMBL2464378; CHEMBL545547
CHEBI: CHEBI:51231
ChemSpider: 2848; 123458; 140783
PubChem: 2954; 21924871; 67094309; 160166

Validation Images & Assay Conditions

Hello CJ!

AR1176 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Long non-coding RNA FOXD3-AS1 aggravates ischemia/reperfusion injury of cardiomyocytes through promoting autophagy

Microencapsulated Schwann cell transplantation inhibits P2X3 receptor expression in dorsal root ganglia and neuropathic pain

Microencapsulation improves inhibitory effects of transplanted olfactory ensheathing cells on pain after sciatic nerve injury

miR-132 regulates the expression of synaptic proteins in APP/PS1 transgenic mice through C1q

Uhrf1-Mediated Tnf-α Gene Methylation Controls Proinflammatory Macrophages in Experimental Colitis Resembling Inflammatory Bowel Disease

Abnormally elevated ubiquilin‑1 expression in breast cancer regulates metastasis and stemness via AKT signaling

Transcriptome sequencing analysis of mono‑ADP‑ribosylation in colorectal cancer cells

Utilization of E-cadherin by monocytes from tumour cells plays key roles in the progression of bone invasion by oral squamous cell carcinoma

Anti-c-Met antibody bioconjugated with hollow gold nanospheres as a novel nanomaterial for targeted radiation ablation of human cervical cancer cell

Neural stem cell transplantation improves learning and memory by protecting cholinergic neurons and restoring synaptic impairment in an amyloid precursor protein/presenilin 1 transgenic mouse model of Alzheimers disease

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2 Customer Q&As for DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining

Question

Do you have a long term storage information about AR1176 and for how long is it stable if aliquoted at 1ug/mL in -20C?

Verified customer

Asked: 2022-07-27

Answer

The DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) For Nucleic Acid Staining (AR1176) is stable at -20C for at least 1 year even if aliquoted at 1ug/mL

Boster Scientific Support

Answered: 2022-07-27

Question

What is the approximate DAPI concentration in this solution?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

The concentration is about 1 µg/mL.

Boster Scientific Support

Answered: 2020-01-01

Size

Total: $15

SKU:AR1176

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