ELISA Technical Resources

Protocols, optimization tips,
troubleshooting guides,
and more for ELISA.

Troubleshooting guides

Troubleshooting guides

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ELISA: How many samples can I run on a plate?

The number of samples that can be tested on each plate will depend on the number of standard points, controls, and replicates you choose to use.

For a 96-well plate, it is common to run an 8-point standard curve with duplicate wells. The remaining 80 wells can be used for controls and/or samples. If controls are not included your assay, 80 samples can be run in singlicate. However, we strongly recommend testing ELISA samples in duplicate or triplicate because this will generate enough data for statistical validation of the results. If samples are tested in duplicates without controls, 40 samples can be run for one ELISA plate.

Keywords: Number of Samples, Microtiter Plate, Maximum Usage