HRP Conjugated Streptavidin

Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity. Cited in 10 publication(s).

Product Info Summary

SKU: BA1088
Size: 0.5ml
Application: ELISA, IHC, WB

Product NameHRP Conjugated Streptavidin
SKU/Catalog NumberBA1088
Pack Size0.5ml
FormConcentrated, Liquid
Concentration1mg/ml
Product DescriptionStreptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-peroxidase can form a complex which composed by about one hundred peroxidase and fifty streptavidin. And lots of enzymes can ensure the streptavidin-peroxidase with high sensitivity.
Contents0.5 mg of HRP conjugated streptavidin
0.01 M PBS (pH7.4)
50% glycerol
Labeling MethodStreptavidin is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.
(Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).)
StorageAt -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
ApplicationsWB, IHC, ELISA
Cite This ProductPeroxidase Conjugated Streptavidin (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1088)

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blotting: 0.25-0.5μg/ml (ECL detection) Western Blotting: 2.5-5μg/ml (DAB detection) Immunohistochemistry: 2-10μg/ml Direct ELISA: 0.01-0.1μg/ml (TMB detection)

Validation Images & Assay Conditions

Hello CJ!

BA1088 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Propofol alleviates hepatic ischemia/reperfusion injury via the activation of the Sirt1 pathway

The protective role of Schwann cells in bladder smooth muscle cell fibrosis

Clinicopathological correlation analysis of (lymph) angiogenesis and corneal graft rejection

Evidence for the Presence of Long-Lived Plasma Cells in Nasal Polyps

Effects of long non-coding RNA myocardial infarction-associated transcript on retinal neovascularization in a newborn mouse model of oxygen-induced retinopathy

A mimetic peptide of α2,6-sialyllactose promotes neuritogenesis

Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway

Expression and prognostic value of E2F3 transcription factor in non‑small cell lung cancer

Exosomes derived from endoplasmic reticulum‑stressed liver cancer cells enhance the expression of cytokines in macrophages via the STAT3 signaling pathway

Protective effects of ginsenoside Rg1 on aging Sca‑1+ hematopoietic cells

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