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Product Info Summary
| SKU: | BA1088 |
|---|---|
| Size: | 0.5ml |
| Application: | ELISA, IHC, WB |
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Product info
| Product Name | HRP Conjugated Streptavidin |
| SKU/Catalog Number | BA1088 |
| Pack Size | 0.5ml |
| Form | Concentrated, Liquid |
| Concentration | 1mg/ml |
| Product Description | Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-peroxidase can form a complex which composed by about one hundred peroxidase and fifty streptavidin. And lots of enzymes can ensure the streptavidin-peroxidase with high sensitivity. |
| Contents | 0.5 mg of HRP conjugated streptavidin 0.01 M PBS (pH7.4) 50% glycerol |
| Labeling Method | Streptavidin is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK. (Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).) |
| Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. |
| Shipping | Ships in room temperature. Can also ship with gel ice or dry ice but not necessary. |
| Applications | WB, IHC, ELISA |
| Cite This Product | Peroxidase Conjugated Streptavidin (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1088) |
Product Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blotting: 0.25-0.5μg/ml (ECL detection) Western Blotting: 2.5-5μg/ml (DAB detection) Immunohistochemistry: 2-10μg/ml ELISA: 0.01-0.1μg/ml (TMB detection)
Validation Images & Assay Conditions
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Boster Kit Box
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