Click image to see more details
Product Info Summary
| SKU: | EK0434 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates and serum. |
Product info
Product Name
Human Laminin ELISA Kit PicoKine®
SKU/Catalog Number
EK0434
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Laminin ELISA Kit PicoKine™ (96 Tests). Quantitate Human Laminin in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Laminin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0434)
Clonality of Antibodies
See Datasheet for details
Immunogen
Target protein isolated from human fibroblasts
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0434 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0434 is reactive to in Human samples
Validated Sample Types
cell culture supernatants, cell lysates and serum.
Application Guarantee
EK0434 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of
Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0434-CAP | Anti-Human Pre-coated 96-well strip microplate | 1 |
| EK0434-ST | Human Standard | 2 vials, 10 ng/tube |
| EK0434-DA | Human Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.095 | 0.137 | 0.168 | 0.247 | 0.425 | 0.624 | 1.284 | 2.010 |
Data Example Images
Click image to see more details
Human Laminin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, Dilution ratio of 1:10, concentration in serum is 5-9 ng/ml..
Some articles we found to cite concentrations of in samples: 20593050 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Laminin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 318 | 1051 | 4462 | 295 | 1015 | 4289 |
| Standard deviation | 23.21 | 47.29 | 348.03 | 26.25 | 58.87 | 411.74 |
| CV (%) | 7.3% | 4.5% | 7.8% | 8.9% | 5.8% | 9.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 318 | 364 | 314 | 373 | 342 | 26.47 | 7.7% |
| Sample 2 | 1051 | 1242 | 1122 | 1215 | 1157 | 75.9 | 6.5% |
| Sample 3 | 4462 | 4109 | 4606 | 4544 | 4430 | 192.37 | 4.3% |
Specific Publications For Human Laminin ELISA Kit PicoKine® (EK0434)
Hello CJ!
EK0434 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
High fibrosis indices in cerebrospinal fluid of patients with shunt-dependent post-traumatic chronic hydrocephalus
Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction
Cytotoxic sesquiterpenoids against hepatic stellate cell line LX2 from Artemisia lavandulaefolia
High fibrosis indices in cerebrospinal fluid of patients with shunt-dependent post-traumatic chronic hydrocephalus
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human Laminin ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
1 Reviews For Human Laminin ELISA Kit PicoKine®
0
Easy to Use Human Laminin ELISA Kit--John Ong, University of Cambridge Engineering Graduate Student
Excellent
Source: Biocompare.com
| Application | Testing laminin concentration in hydrogel |
|---|---|
| Starting Material | Hydrogel |
| Tips | Supernatant used for ELISA |
"Positive:(1) Very straight forward and easy to use kits.(2) Accurate results(3) Removable well-strips so experiments can be staggered.Negative:(1) Price point - could be cheaper(2) Reagents have to be used within 1 month upon defrosting."
John Ong
Verified customer
Submitted 2020-07-30
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Human Laminin ELISA Kit PicoKine®
Question
Would EK0434 work for the quantification of laminin 332 (or laminin-5) in the cell supernatant?
Verified customer
Asked: 2021-02-01
Answer
The Human Laminin ELISA Kit PicoKine™ (EK0434) detects total laminin and it is not specific to any isoform like laminin 332 or laminin-5.
Boster Scientific Support
Answered: 2021-02-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-05-09
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-05-09
Question
Q: which procedure should I follow in order to thaw whole blood sample for LAMA1; LAMB1; LAMC1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-05-09
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test LAMA1; LAMB1; LAMC1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-05-09
Question
Q: can you recommend the dilution ratio of serum samples for detection of LAMA1; LAMB1; LAMC1 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the LAMA1; LAMB1; LAMC1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-01-31
Question
Which subunits of laminin does this kit recognize? Keywords: chain, isoform, subunits
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Our Human Laminin PicoKine™ ELISA Kit EK0434 is the Laminin pan specific to all Laminin subunits. The kit can recognize alpha, beta, and gamma subunits of laminin.
Boster Scientific Support
Answered: 2019-11-25
Question
Has EK0434 been tested for condition media in samples?
Verified customer
Asked: 2019-08-19
Answer
Unfortunately, we have not tested the Human Laminin ELISA Kit PicoKine™ (EK0434) for condition media, so we cannot guarantee that would work. In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.
Boster Scientific Support
Answered: 2019-08-19
Question
Would EK0434 kit be suitable for detection of laminin 1, 2 and 8 (Either beta-1 or gamma-1) and which chain or isomer is this kit specific for?
Verified customer
Asked: 2019-08-16
Answer
The Human Laminin ELISA Kit PicoKine EK0434 is able to detect all forms of Laminin. It is not isoform specific.
Boster Scientific Support
Answered: 2019-08-16
Question
Are the capture and detection antibodies for EK0434 rabbit polyclonals? Do they have the same immunogen and are they both towards the native human protein?
Verified customer
Asked: 2019-05-03
Answer
Both capture and detection antibodies of the Human Laminin ELISA Kit PicoKine™ (EK0434) have the same immunogen. They are both towards the native human protein.
Boster Scientific Support
Answered: 2019-05-03
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring LAMA1; LAMB1; LAMC1 using the EK0434 Human LAMA1; LAMB1; LAMC1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-01-20
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-01-20
Question
My main concert is if this kit would be suitable for the laminin isomer I will be using it for. Which chain/isomer is this kit specific for?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
EK0434 is able to detect all forms of Laminin. It is not isoform specific.
Boster Scientific Support
Answered: 2018-10-01
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-02-06
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-02-06
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
L. Gandhi
Verified customer
Asked: 2016-08-04
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-08-04
Question
The protocol for EK0434 states that PBS or TBS should be used as wash buffer. Is there any difference between PBS and TBS (i.e. depending on sample type)?
Verified Customer
Verified customer
Asked: 2014-09-04
Answer
PBS and TBS are only different in recipes. You are free to choose either of them.
Boster Scientific Support
Answered: 2014-09-04
