Polymerase Chain Reaction (PCR) Technical Resources

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Technical Resources Overview

PCR Principle

Molecular biology has long been the basis for the understanding of each individual step in the biology central dogma: DNA replication, DNA transcription into RNA, and RNA translation into proteins. Manipulation and investigation of genetic material is done through various techniques including traditional molecular cloning (library construction), Polymerase Chain Reaction (PCR), and gel electrophoresis. These molecular biology techniques have various broad and useful applications in our scientific community, so understanding the fundamental principles will be useful in your journey.

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PCR Sample Preparation

Every experiment begins with sample preparation. We have included the standard operating procedures for the basic techniques of molecular biology. If you need instructions for DNA and RNA extraction and purification or for molecular cloning (DNA library preparation), you have come to the right place. Use our step-by-step guides as reference, and be sure to check the product datasheets for specific details.

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PCR Protocols

Your experiment requires a solid protocol. We have provided guidelines for designing primers and performing polymerase chain reaction. If you're looking for protocols of PCR, reverse transcription, and qRT-PCR, we've posted step-by-step guides for your reference. Be sure to check the product datasheets for specific details. Following an optimized protocol will lead to better and more reproducible data results.

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PCR Troubleshooting Tips

An unsuccessful experiment can be disheartening, but we have provided here a simplified table for solving your troubles. If you encounter problems such as low yields or contamination when extracting DNA and RNA, we have some common solutions to help. If you have no idea why your PCR experiment failed, then check our troubleshooting table for advice on how to fix your specific problem.

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