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Molecular Biology Resource Center

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Molecular Biology Technical Resources

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Principles of Molecular Biology

Molecular biology has long been the basis for the understanding of each individual step in the biology central dogma: DNA replication, DNA transcription into RNA, and RNA translation into proteins. Manipulation and investigation of genetic material is done through various techniques including traditional molecular cloning (library construction), Polymerase Chain Reaction (PCR), and gel electrophoresis. These molecular biology techniques have various broad and useful applications in our scientific community, so understanding the fundamental principles will be useful in your journey.

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Molecular Biology Sample Preparation and Protocols

Every experiment begins with a solid protocol, and here we have included the standard operating procedures for the basic techniques of molecular biology. If you need instructions for DNA and RNA extraction and purification, for molecular cloning, or Polymerase Chain Reaction (PCR) and primer design, then you have come to the right place. Use our step-by-step guides as reference, and be sure to check the product datasheets for specific details. Following an optimized protocol will lead to better and more reproducible data results.

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Molecular Biology Troubleshooting Guide

An unsuccessful experiment can be disheartening, but we have provided here a simplified table for solving your troubles. If you encounter problems such as low yields or contamination when extracting DNA and RNA, we have some common solutions to help. If you have no idea why your PCR experiment failed, then check our troubleshooting table for advice on how to fix your specific problem.

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Molecular Biology FAQs

Molecular biology is a complex and broad field of science. Read the answers to common questions that all molecular biologists have so you will be prepared before you dive in. Our FAQ will guide you to make informed decisions, optimize your experiments, and get better results. Feel free to contact us with any further questions, and our support team will respond within 24 hours.

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