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SKU AR0008
Pack size 12 mL(for 240 assays)
Applications Counterstain in IHC (BCIP/NBT chromogenic reaction)

Overview

Product Name Nuclear Fast Red
SKU/Catalog Number AR0008
Pack Size 12 mL(for 240 assays)
Form Supplied Solution
Format Ready-to-Use
Concentration 0.1% Nuclear Fast Red in distilled water
Appearance Red
Storage Upon receipt store at 4°C for one year.
Application Counterstain in IHC (BCIP/NBT chromogenic reaction)
Equivalent Vector Labs (Product No. H-3403), Millipore Sigma (Product No. N 8002)
Description Boster’s Nuclear Fast Red is a rapid staining solution which is a popular alternative to hematoxylin and typically stains nuclei in five minutes.
Cite This Product Nuclear Fast Red (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0008)

Chemical structures

Molecular formula: C14H8NNaO7S

Molecular weight: 357.28

CAS Number: 6409-77-4

Background

Nuclear Fast Red is suitable for the determination of calcium. It is suitable for use in prussian blue staining in neural progenitor cells. It may be employed as highly sensitive "off/on" fluorescent probe for the determination of guanine. Nuclear fast red (2-anthracenesulfonic acid, 4-amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-, monosodium salt) may be used as selective complexing agent for the simultaneous determination of copper and bismuth by adsorptive stripping voltammetry.
Boster’s Nuclear Fast Red is an optimized nuclear counterstain solution that produces an intense nuclear stain. It is an anionic dye used in conjunction with an aluminium salt as a mordant. In contrast to hematoxylin, Nuclear Fast Red is a rapid staining procedure, which requires only step and stains nucleus in five minutes. It is an excellent alternative in multiple labeling when hematoxylin would otherwise obscure the substrate colors.

Protocol

1. Rinse sections in distilled water.

2. Add 50 μl Nuclear Fast Red directly onto the tissue section and incubate for 5 minutes.

3. Wash with distilled water for 1 minute.

Nuclear Fast Red Images

Click the images to enlarge.

Nuclear Fast Red
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Publications

A potent human neutralizing antibody Fc-dependently reduces established HBV infections
Li D, He W, Liu X, Zheng S, Qi Y, Li H, Mao F, Liu J, Sun Y, Pan L, Du K, Ye K, Li W, Sui J. Elife. 2017 Sep 26;6. pii: e26738. doi: 10.7554/eLife.26738. A potent human neutralizing antibody Fc-dependently reduces established HBV infections
Zhao JR, Cheng WW, Wang YX, Cai M, Wu WB, Zhang HJ. Cell Death Dis. 2018 Jan 24;9(2):94. doi: 10.1038/s41419-017-0108-2. Identification of microRNA signature in the progression of gestational trophoblastic disease.
Ni W, Zhang Y, Zhan Z, Ye F, Liang Y, Huang J, Chen K, Chen L, Ding Y. J Hematol Oncol. 2017 Apr 19;10(1):91. doi: 10.1186/s13045-017-0449-4. A novel lncRNA uc.134 represses hepatocellular carcinoma progression by inhibiting CUL4A-mediated ubiquitination of LATS1.