Nuclear Fast Red


Nuclear Fast Red nuclear counterstain solution is a rapid staining procedure which is a popular alternative to hematoxylin and typically stains nuclei in five minutes. IHC related Cited in 5 publication(s).

Product Info Summary

SKU: AR0008
Size: From 12mL to 100 mL


Product Name Nuclear Fast Red
Form Supplied Solution
Format Ready-to-Use
Concentration 0.1% Nuclear Fast Red in distilled water
Appearance Red
Storage Upon receipt store at 4°C for one year.
Application Counterstain in IHC (BCIP/NBT chromogenic reaction)
Equivalent Vector Labs (Product No. H-3403), Millipore Sigma (Product No. N 8002)
Description Boster’s Nuclear Fast Red is a rapid staining solution which is a popular alternative to hematoxylin and typically stains nuclei in five minutes.

Chemical structures

Molecular formula: C14H8NNaO7S

Molecular weight: 357.28

CAS Number: 6409-77-4


Nuclear Fast Red is suitable for the determination of calcium. It is suitable for use in prussian blue staining in neural progenitor cells. It may be employed as highly sensitive "off/on" fluorescent probe for the determination of guanine. Nuclear fast red (2-anthracenesulfonic acid, 4-amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-, monosodium salt) may be used as selective complexing agent for the simultaneous determination of copper and bismuth by adsorptive stripping voltammetry.
Boster’s Nuclear Fast Red is an optimized nuclear counterstain solution that produces an intense nuclear stain. It is an anionic dye used in conjunction with an aluminium salt as a mordant. In contrast to hematoxylin, Nuclear Fast Red is a rapid staining procedure, which requires only step and stains nucleus in five minutes. It is an excellent alternative in multiple labeling when hematoxylin would otherwise obscure the substrate colors.


1. Rinse sections in distilled water.

2. Add 50 μl Nuclear Fast Red directly onto the tissue section and incubate for 5 minutes.

3. Wash with distilled water for 1 minute.

Validation Images & Assay Conditions

AR0008 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation

Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation

Li D, He W, Liu X, Zheng S, Qi Y, Li H, Mao F, Liu J, Sun Y, Pan L, Du K, Ye K, Li W, Sui J. Elife. 2017 Sep 26;6. pii: e26738. doi: 10.7554/eLife.26738. A potent human neutralizing antibody Fc-dependently reduces established HBV infections

Zhao JR, Cheng WW, Wang YX, Cai M, Wu WB, Zhang HJ. Cell Death Dis. 2018 Jan 24;9(2):94. doi: 10.1038/s41419-017-0108-2. Identification of microRNA signature in the progression of gestational trophoblastic disease.

Ni W, Zhang Y, Zhan Z, Ye F, Liang Y, Huang J, Chen K, Chen L, Ding Y. J Hematol Oncol. 2017 Apr 19;10(1):91. doi: 10.1186/s13045-017-0449-4. A novel lncRNA uc.134 represses hepatocellular carcinoma progression by inhibiting CUL4A-mediated ubiquit...

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1 Customer Q&As for Nuclear Fast Red


Is this the same nuclear fast red counter stain for IHC's?

Verified customer

Asked: 2019-05-30


Yes, our Nuclear Fast Red (AR0008) is for Counterstaining in IHC (BCIP/NBT chromogenic reaction).

Boster Scientific Support

Answered: 2019-05-30


Total: $15


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