Boster offers the full range of reagents one needs for performing PCR, RT-PCR and qPCR. From extraction and purification of DNA and RNA, to polymerase chain reaction and reverse transcriptase PCR (RT-PCR), Boster provides convenient solutions to save your time and maximize reproducibility. Our popular miniprep kits for DNA/RNA purification and PCR master mix kits provide simple, time-saving protocols, and reduces contamination by minimizing the number of steps for PCR set up. Amplify DNA from any template efficiently and accurately, with the assurance that every product is covered by the Boster Guarantee.
PCR reaction requires multiple components to run. Some of these components are not sample-specific. A PCR mastermix is a premixed solution (and Boster offers it as both solution and lyophilized, pellet-in-PCR-tube, format) with all such components mixed in and ready to use. The exact components include DNA polymerase, dNTPs, MgCl2, and some additives in a buffer optimized for PCR. The same goes for RT-PCR and qPCR. To use a master mix for your PCR experiment, simply add template, primers, probes (if being used), and purified free water if needed. In real-time or quatitative PCR (qPCR), one or more dyes are added for quantitative measurement of mRNA.
Evaluation of primer pair performance for probe-based real-time PCR
qPCR PreMix is a kit for providing accurate data from your various samples speedily. There are no additional mixing steps because it already prepared and dried for your convenient. This product makes you monitor your gene amplification of Ct in every steps of amplified florescent cycle because we add “probe” which include florescence oligo-nucleotide and primers.
A plate of 12 individual 8-tube strips filled with a lyophilized pellet of PCR Master Mix. This innovative format saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up..
A plate of 12 individual 8-tube strips filled with a lyophilized pellet of PCR Master Mix. This innovative format saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. This version is without loading dye.