PCR master mix and reagents

Boster offers the full range of reagents one needs for performing PCR, RT-PCR and qPCR. From extraction and purification of DNA and RNA, to polymerase chain reaction and reverse transcriptase PCR (RT-PCR), Boster provides convenient solutions to save your time and maximize reproducibility. Our popular miniprep kits for DNA/RNA purification and PCR master mix kits provide simple, time-saving protocols, and reduces contamination by minimizing the number of steps for PCR set up. Amplify DNA from any template efficiently and accurately, with the assurance that every product is covered by the Boster Guarantee.

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What is PCR master mix

PCR reaction requires multiple components to run. Some of these components are not sample-specific. A PCR mastermix is a premixed solution (and Boster offers it as both solution and lyophilized, pellet-in-PCR-tube, format) with all such components mixed in and ready to use. The exact components include DNA polymerase, dNTPs, MgCl2, and some additives in a buffer optimized for PCR. The same goes for RT-PCR and qPCR. To use a master mix for your PCR experiment, simply add template, primers, probes (if being used), and purified free water if needed. In real-time or quatitative PCR (qPCR), one or more dyes are added for quantitative measurement of mRNA.



Displaying 7 Items

    qPCR PreMix Kit (MB1001-2)

    SKU:MB1001-2
    Application:Gene expression profiling
    Target DNA quantification
    Microbial detection
    Viral/bacterial pathogen load determination
    Evaluation of primer pair performance for probe-based real-time PCR
    Description:qPCR PreMix is a kit for providing accurate data from your various samples speedily. There are no additional mixing steps because it already prepared and dried for your convenient. This product makes you monitor your gene amplification of Ct in every steps of amplified florescent cycle because we add “probe” which include florescence oligo-nucleotide and primers.

    Miniprep Kit For Plasmid DNA Extraction And Purification (MB1005)

    • Q&As (1)
    SKU:MB1005
    Application:PCR DNA extraction
    Description:Miniprep kit for plasmid DNA extraction

    Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye (MB1004)

    SKU:MB1004
    Application:qPCR (cDNA synthesis + qPCR in one reaction)
    Description:This Kit contains all the reagents necessary for first‐strand cDNA synthesis and qPCR, without the template.

    One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction) (MB1003)

    SKU:MB1003
    Application:first‐strand cDNA synthesis and PCR
    Description:This Kit contains all the reagents necessary for first‐strand cDNA synthesis and PCR, without the template.

    Reverse Transcriptase RT PCR Master Mix (5X) (MB1002)

    SKU:MB1002
    Application:first‐strand cDNA synthesis
    Description:This Master Mix contains all reagents needed for first‐strand cDNA synthesis except the templates. Downstream applications include qRT-PCR.

    PCR Master Mix, Ready To Load (96 well, 12x8 strip-well plate, lyophilized with loading dye) (MB1001)

    SKU:MB1001
    Application:PCR
    Description:A plate of 12 individual 8-tube strips filled with a lyophilized pellet of PCR Master Mix. This innovative format saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up..

    PCR Master Mix, Ready To Load (96 well, 12x8 strip-well plate, lyophilized without loading dye) (MB1001-1)

    SKU:MB1001-1
    Application:PCR
    Description:A plate of 12 individual 8-tube strips filled with a lyophilized pellet of PCR Master Mix. This innovative format saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. This version is without loading dye.

Displaying 7 Items