Anti-NELF NSMF Antibody

Product Name

Anti-NELF NSMF Antibody

View all NELF Antibodies

SKU/Catalog Number

A08385

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NELF NSMF Antibody (Catalog # A08385). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

NELF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-NELF NSMF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08385)

Host

Rabbit

Contents

NELF Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Rabbit polyclonal NELF antibody was raised against a 17 amino acid peptide near the center of human NELF. The immunogen is located within amino acids 230 - 280 of NELF.

Reactive Species

A08385 is reactive to NELF in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

60.1 kDa

Background of NELF

NELF (nasal embryonic luteinizing hormone-releasing hormone factor) is a 530 amino acid transcription factor involved in the migration of LHRH neurons, outgrowth of olfactory axons and suppression of transcription elongation. NELF is found in the peripheral and central nervous system during embryonic development, and is highly expressed in adult testis, kidney and brain. Known to couple NMDA receptor signaling to the nucleus, NELF knockdown impaired GnRH neuronal migration of NLT cells in vitro and the gene encoding NELF has been linked to the development of Idiopathic hypogonadotropic hypogonadism (IHH), a disorder resulting in impaired pubertal maturation and reproductive function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08385 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NELF

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