Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody

Product Name

Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody

View all TUBA1 Antibodies

SKU/Catalog Number

M03989-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (Catalog# M03989-1). Tested in IP, WB, IHC-P, ICC, ELISA application(s). This antibody reacts with Mouse, Rat, Pig, Nicotiana, Human, Dog.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03989-1)

Host

Mouse

Contents

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

TU-16

Isotype

Mouse IgM

Immunogen

Porcine brain microtubule protein MTP-1. The antibody TU-16 reacts with alpha-tubulin of all tested species, under denaturing and non-denaturing conditions.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M03989-1 is reactive to TUBA1 in Dog, Human, Mouse, Pig, Rat, Nicotiana

Observed Molecular Weight

42 kDa

Calculated molecular weight

50.1 kDa

Background of TUBA1

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –; this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03989-1 is guaranteed for ELISA, IP, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 10 μg/ml.
Immunoprecipitation: Reducing conditions.
Western blotting: 1-2 μg/ml. This antibody can be used for Western blotting, but its alternative TU-02 (11-447-C100) gives better signal in this application.

Validation Images & Assay Conditions

See full target information TUBA1

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