Anti-Alpha-tubulin TUBA1A Antibody (biotin)

Product Name

Anti-Alpha-tubulin TUBA1A Antibody (biotin)

View all TUBA1A Antibodies

SKU/Catalog Number

A03989-Biotin

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Alpha-tubulin TUBA1A Antibody (biotin) (Catalog # A03989-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken, Zebrafish.

Storage & Handling

Biotin-Alpha-tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Alpha-tubulin TUBA1A Antibody (biotin) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03989-Biotin)

Host

Rabbit

Contents

Biotin-Alpha-tubulin antibody is supplied in PBS containing 1% stabilizing protein and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Biotin-Alpha-tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-tubulin

Cross-reactivity

Biotin-Alpha-tubulin antibody is human, mouse, rat, rabbit, chicken, and zebrafish reactive.

Reactive Species

A03989-Biotin is reactive to TUBA1A in Chicken, Human, Mouse, Rabbit, Rat, Zebrafish

Observed Molecular Weight

68 kDa

Calculated molecular weight

50.1 kDa

Background of TUBA1A

Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and -Actin, this antibody makes an excellent loading control in immunoblots.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03989-Biotin is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Biotin-Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TUBA1A

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