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Product Info Summary
| SKU: | A00227-Biotin |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Chicken, Human, Mouse, Rabbit, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GAPDH Antibody (biotin)
SKU/Catalog Number
A00227-Biotin
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GAPDH Antibody (biotin) (Catalog # A00227-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken.
Storage & Handling
Biotin-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-GAPDH Antibody (biotin) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00227-Biotin)
Host
Rabbit
Contents
Biotin-GAPDH antibody is supplied in PBS containing 1% stabilizing protein and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Biotin-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
Cross-reactivity
Biotin-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.
Reactive Species
A00227-Biotin is reactive to GAPDH in Chicken, Human, Mouse, Rabbit, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
36.1 kDa
Background of GAPDH
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00227-Biotin is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 μ/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GAPDH in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT4, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, mouse lung, mouse liver, rat liver, and chicken small intestine lysate with Biotin-GAPDH antibody at 1 μg/mL.
Specific Publications For Anti-GAPDH Antibody (biotin) (A00227-Biotin)
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