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Product Info Summary
| SKU: | H00227 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated
SKU/Catalog Number
H00227
BM3896 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated catalog # H00227. Tested in WB application. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H00227)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
A-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GAPDH (HRP conjugated)
Reactive Species
H00227 is reactive to GAPDH in Human, Monkey, Mouse, Rat
Observed Molecular Weight
36 kDa
Calculated molecular weight
36.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
H00227 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
Positive Control
WB: Jurkat cell, A375 cell, Human hippocampus, Human fetal liver, COS-1 cell, Raw2647 cell, Mouse kidney, PC-12 cell, Rat brain
Validation Images & Assay Conditions
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Western blot analysis of GAPDH expression in (1) Jurkat cell lysate; (2) A375 cell lysate; (3) Human hippocampus lysate; (4) Human fetal liver lysate; (5) COS-1 cell lysate; (6) Raw264.7 cell lysate; (7) Mouse kidney lysate; (8) PC-12 cell lysate; (9) Rat brain lysate
Specific Publications For Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated (H00227)
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4 Customer Q&As for Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated
Question
We have seen staining in mouse lung. Any tips? Is anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
From literature lung does express GAPDH. From Uniprot.org, GAPDH is expressed in smooth muscle tissue, liver, lung, placenta, astrocytoma, leukemia, colon adenocarcinoma, eye, kidney, lung, lymph placenta, muscle, platelet, prostatic carcinoma, brain, cajal-retzius cell fetal brain cortex, heart, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305
Boster Scientific Support
Answered: 2020-04-28
Question
My team were satisfied with the WB result of your anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated. However we have observed positive staining in lymphoblast cytosol using this antibody. Is that expected? Could you tell me where is GAPDH supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
From literature, lymphoblast does express GAPDH. Generally GAPDH expresses in cytoplasm, cytosol. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305
Boster Scientific Support
Answered: 2018-06-13
Question
We are currently using anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated H00227 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
The anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated (H00227) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-19
Question
We need using your anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated for killing of cells of other organism studies. Has this antibody been tested with western blotting on mouse kidney? We would like to see some validation images before ordering.
R. Huang
Verified customer
Asked: 2016-01-19
Answer
I appreciate your inquiry. This H00227 anti-GAPDH Rabbit Monoclonal antibody, HRP Conjugated is tested on jurkat cell lysate, mouse kidney. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-01-19
