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GAPDH antibody (H00227) performs well in WB and shows reactivity with Human, Monkey, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 16 Publications

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H00227 GAPDH primary antibodies WB testing 1

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Western blot analysis of GAPDH expression in (1) Jurkat cell lysate; (2) A375 cell lysate; (3) Human hippocampus lysate; (4) Human fetal liver lysate; (5) COS-1 cell lysate; (6) Raw264.7 cell lysate; (7) Mouse kidney lysate; (8) PC-12 cell lysate; (9) Rat brain lysate

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Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated

GAPDH antibody

16 Publications

Reactive Species	Host	Application
Human, Monkey, Mouse, Rat	Rabbit	WB

Tested Applications

GAPDH antibody was positively detected by WB in:

Jurkat cell, A375 cell, Human hippocampus, Human fetal liver, COS-1 cell, Raw2647 cell, Mouse kidney, PC-12 cell, Rat brain

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

Human

Tested

Mouse

Tested

Rat

Tested

Product Information

Product Name

Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated
View all GAPDH Antibodies

Form

Liquid

Uniprot

P04406

Gene ID

2597

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

A-7

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human GAPDH (HRP conjugated)

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

36 kDa

Calculated Molecular Weight

36.1 kDa

Cite This Product

Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H00227)

Protocols

Western Blot protocol for GAPDH antibody

Review Protocol

Publications

A cell-free browning strategy: Exosomal miR-21a-5p from ADSCs targets PDCD4 to reshape adipose metabolism

Published: 2026-07-14

Journal: iScience

Application: WB

Impact Factor: 4.50

Species: Mouse

PMID: 42519062

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Free Secondary Antibody

Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated

GAPDH antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

A cell-free browning strategy: Exosomal miR-21a-5p from ADSCs targets PDCD4 to reshape adipose metabolism

Bioinformatic and experimental analysis reveals ITGA5 as a driver of ovarian clear cell carcinoma progression and platinum resistance

Heme oxygenase 1‑overexpressing bone marrow mesenchymal stem cell‑derived exosomes suppress interleukin‑1 beta‑induced apoptosis and aging of nucleus pulposus cells

IFI-16 inhibition attenuates myocardial remodeling following myocardial infarction

Electrochemical detection mechanism of estrogen effect induced by cadmium: The regulation of purine metabolism by the estrogen effect of cadmium

The response of electrochemical method to estrogen effect and the tolerance to culture factors: Comparison with MTT and cell counting methods

Genistein-induced mitochondrial dysfunction and FOXO3a/PUMA expression in non-small lung cancer cells

Serotonin system is partially involved in immunomodulation of Nile tilapia (Oreochromis niloticus) immune cells

Normal mesenchymal stem cells can improve the abnormal function of T cells in psoriasis via upregulating transforming growth factor-β receptor

SP protects Nile tilapia (Oreochromis niloticus) against acute Streptococcus agalatiae infection

MicroRNA‑203‑3p inhibits the proliferation, invasion and migration of pancreatic cancer cells by downregulating fibroblast growth factor 2

Human Umbilical Cord Mesenchymal Stem Cells Attenuate Abdominal Aortic Aneurysm Progression in Sprague-Dawley Rats: Implication of Vascular Smooth Muscle Cell Phenotypic Modulation

Metformin attenuates cardiac remodeling in mice through the Nrf2/Keap1 signaling pathway

Exenatide alleviates mitochondrial dysfunction and cognitive impairment in the 5×FAD mouse model of Alzheimer’s disease

Downregulation of syndecan‑1 expression induces activation of hepatic stellate cells via the TGF‑β1/Smad3 signaling pathway

Inhibition of autophagy via activation of PI3K/Akt/mTOR pathway contributes to the protection of hesperidin against myocardial ischemia/reperfusion injury

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