Product Info Summary
SKU: | AR4011 |
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Size: | 50 Assays |
Product info
Overview
Product Name | Protease Activity Assay Kit |
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SKU/Catalog Number | AR4011 |
Description | Proteases are enzymes, naturally present in all living cells and tissues, involved in a multitude of physiological reactions that includes digestions of proteins in the food to highly regulated cascades. Proteases can either break specific peptide bonds, depending on the amino acid sequence of a protein or completely breakdown peptides to amino acids. In certain physiological conditions, protease activity can abolish a protein's function or an activation of a function. For studying proteins, tissues and cells are lysed, which release proteases in the sample and their activities can be measured using a protease assay. Cepham Life Sciences, protease assay kit is a sensitive, universal protease assay kit, which is designed for the quantitative determination of the proteases present in the protein sample and can detect approximately 10ng/ml of proteases in 2 to 24 hours incubation. The kit is supplied with a protease substrate: casein labeled with resorufin and with protease activities in the test sample, resorufin-labeled peptides are released, that cannot be precipitated by the precipitating agent. The concentration of these resorufin-labeled peptides in the supernatant is directly proportional to the proteolytic activity present in the test sample, which can be measured spectrophotometrically at 574nm. |
Cite This Product | Protease Activity Assay Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR4011) |
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2 Customer Q&As for Protease Activity Assay Kit
Question
How many reactions AR4011 is suitable for?
Verified customer
Asked: 2022-04-26
Answer
The Protease Activity Assay Kit (AR4011) is suitable for 50 assays.
Boster Scientific Support
Answered: 2022-04-27
Question
Should AR4011 be used with a micropate reader or a cuve-spectrophotometer ?
Verified customer
Asked: 2021-01-04
Answer
The Protease Activity Assay Kit (AR4011) is designed for Microplate Reader.
Boster Scientific Support
Answered: 2021-01-04