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SKU AR0105
Pack size 50ml
Form Solution (liquid)
Applications Western blotting, ELISA, immunofluorescence, Immunoprecipitation; protein purification; SDS-PAGE; protein assays; epitope tagging; reporter gene analysis
Price: $25.00
Quantity:

Overview

Product Name RIPA Lysis Buffer
Synonyms RIPA buffer; Radio-immunoprecipitation assay buffer, RIPA Lysis and Extraction Buffer
Description RIPA Lysis Buffer is a complete cell lysis solution reagent used for rapid and efficient total cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells, effectively extracting cytoplasmic, nuclear and membrane proteins. RIPA lysis buffer is highly compatible with various downstream protein analysis applications.
Application Western blotting, ELISA, immunofluorescence, Immunoprecipitation; protein purification; SDS-PAGE; assays for protein expression, activity, modification, profiling, characterization and quantitative measurement; epitope tagging; reporter gene analysis
Pack Size 50 ml
Reagent Type Western Blotting related reagent; Cell lysis buffer; Universal tissue extraction buffer; Detergent solution
Content 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SDS
Usage Extraction of cytoplasmic, membrane and nuclear proteins

Properties

Form Supplied Ready-to-use 1X solution
Physical State Liquid
Pack Size 50 ml
Recommended working concentration 10 ml RIPA Lysis Buffer per gram of tissue
1 ml RIPA Lysis Buffer per 5.0x105 cells in suspension
1 ml RIPA Lysis Buffer per 5.0x105 adherent mammalian cells
0.5-1 ml RIPA lysis buffer per subconfluent monolayer on a 100 mm plate
Compatibility with reagents Fully compatible with Broad Spectrum Protease Inhibitor Cocktail and Broad Spectrum Phosphatase Inhibitor Cocktail
Storage Store at 4˚C for one year.

Chemical Information

Component: Effects:
NP-40 Non-ionic detergent to extract proteins
Sodium deoxycholate Ionic detergent to extract membrane proteins
SDS Ionic detergents to extract proteins

Usage and Handling

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. RIPA lysis buffer is highly compatible with immunoassays, protein purification procedures, immunoprecipitation, and western blotting.

Background

RIPA lysis extraction buffer contains non-ionic and ionic detergents which are able to extract protein from wide variety of cell types and membrane structures. RIPA buffer ensures efficient cell lysis and protein solubilization preventing protein degradation and interference with protein immunoreactivity and biological activity. Since most antibodies and protein antigens are not adversely affected by the components of this solution, RIPA buffer-conducted protein extraction is compatible with various downstream immunoprecipitation and molecular pull-down assays. RIPA buffer reagent minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions.

RIPA Lysis Buffer Images

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RIPA Lysis Buffer
RIPA Lysis Buffer (AR0105)
50 mL per pack, Solution (liquid)
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Publications

Tian, J., Liu, J., & Liang, J. (2017). HBx enhances cell viability, migration and invasion of hepatocellular carcinoma via PD-L1. Cancer Cell Research, 4(14), 351-355. Retrieved from http://www.cancercellresearch.org/PDF/20171405.pdf
Hu, Q.X., Li, X.D., Xie, P., Wu, C.C., Zheng, G.Z., Lin, F.X.,…, & Du, S.X. (2017). All-trans-retinoic acid activates SDF-1/CXCR4/ROCK2 signaling pathway to inhibit chondrogenesis. American Journal of Translational Research, 9(5), 2296-2305. Retrieved from http://www.ajtr.org/files/ajtr0048341.pdf
Liu, X., Guo, J., Zhou, M., Yang, Y., Liang, M., Bai, C., Zhao, Z., & Sun, B. (2017). RNA binding protein QKI contributes to WT1 mRNA and suppresses apoptosis in ST cells. Genes & Genomics, 1-8. doi:10.1007/s13258-017-0560-5
Xiao-Lei Xue, Xiao-Dan Han, Yuan Li, Xiao-Fei Chu, Wei-Min Miao, Jun-Ling ZhangEmail author and Sai-Jun FanEmail author Stem Cell Research & Therapy20178:7 DOI: 10.1186/s13287-016-0464-3 Astaxanthin attenuates total body irradiation-induced hematopoietic system injury in mice via inhibition of oxidative stress and apoptosis
Qian Yu, Minchao Li Journal of Biomechanics Available online 9 December 2016 Effects of transient receptor potential canonical 1 (TRPC1) on the mechanical stretch-induced expression of airway remodeling-associated factors in human bronchial epithelioid cells
Gaofeng Liang, Yang Li, Wenpo Feng, Xinshuai Wang, Aihua Jing, Jinghua Li, and Kaiwang Ma Int J Nanomedicine. 2016; 11: 6079–6088. Published online 2016 Nov 15. doi: 10.2147/IJN.S120828 Polyethyleneimine-coated quantum dots for miRNA delivery and its enhanced suppression in HepG2 cells
Tang W, Yang J, Zhang F, Guo H, Peng F, Wang X. Cytotherapy. 2014 Feb;16(2):225-33. Doi: 10.1016/J.Jcyt.2013.08.009. Epub 2013 Oct 8. Activation Of Extracellular Signal-Regulated Kinase 1/2 And Sp1 May Contribute To The Expression Of Tissue Inhibi...
Chen Z, Ma T, Huang C, Zhang L, Zhong J, Han J, Hu T, Li J. Eur J Pharm Sci. 2014 Mar 12;53:69-76. Doi: 10.1016/J.Ejps.2013.12.010. Epub 2013 Dec 21. Efficiency Of Transcellular Transport And Efflux Of Flavonoids With Different Glycosidic Units Fr...
Xiao W, Gao Z, Duan Y, Yuan W, Ke Y. Oncol Rep. 2015 Aug;34(2):739-46. Doi: 10.3892/Or.2015.4041. Epub 2015 Jun 8. Downregulation Of Mir-19A Exhibits Inhibitory Effects On Metastatic Renal Cell Carcinoma By Targeting Pik3Ca And Inactivating Notch ...
Sun Y, Chen H, Ma S, Liang L, Zheng Y, Guo X, Wang M, Wang W, Li G, Zhong D. Neurochem Res. 2015 Jul;40(7):1410-20. Doi: 10.1007/S11064-015-1608-Z. Epub 2015 May 22. Administration Of Sb203580, A P38 Mapk Inhibitor, Reduced The Expression Of Mmp9,...
Wang J, Liao Y, Fan J, Ye T, Sun X, Dong S. Inflammation. 2012 Aug;35(4):1466-76. Doi: 10.1007/S10753-012-9460-7. Apigenin Inhibits The Expression Of Il-6, Il-8, And Icam-1 In Dehp-Stimulated Human Umbilical Vein Endothelial Cells And In Vivo.
Wang Z, Dao R, Bao L, Dong Y, Wang H, Han P, Yue Y, Yu H. J Cell Mol Med. 2014 Sep;18(9):1807-15. Doi: 10.1111/Jcmm.12306. Epub 2014 May 30. Epigenetic Reprogramming Of Human Lung Cancer Cells With The Extract Of Bovine Parthenogenetic Oocytes.
Lv J, Sun X, Ma J, Ma X, Zhang Y, Li F, Li Y, Zhao Z. Biochem Biophys Res Commun. 2015 Aug 14;464(1):263-8. Doi: 10.1016/J.Bbrc.2015.06.140. Epub 2015 Jun 23. Netrin-1 Induces The Migration Of Schwann Cells Via P38 Mapk And Pi3K-Akt Signaling Path...
Dong L, Wang X, Tan J, Li H, Qian W, Chen J, Chen Q, Wang J, Xu W, Tao C, Wang S. J Cell Mol Med. 2014 Nov;18(11):2213-24. Doi: 10.1111/Jcmm.12353. Epub 2014 Aug 28. Decreased Expression Of Microrna-21 Correlates With The Imbalance Of Th17 And Tre...
Wang Yg, Xie P, Wang Yg, Li Xd, Zhang Tg, Liu Zy, Hong Q, Du Sx. Environ Toxicol Pharmacol. 2014 Sep;38(2):460-8. Doi: 10.1016/J.Etap.2014.07.008. Epub 2014 Jul 21. All-Trans-Retinoid Acid (Atra) Suppresses Chondrogenesis Of Rat Primary Hind Limb ...
Guo Sx, Jin Yy, Fang Q, You Cg, Wang Xg, Hu Xl, Han Cm. Plos One. 2015 Apr 13;10(4):E0124897. Doi: 10.1371/Journal.Pone.0124897. Ecollection 2015. Beneficial Effects Of Hydrogen-Rich Saline On Early Burn-Wound Progression In Rats.
Zhou J, Qu C, Sun Q, Wu L, Liu Y, Yang Z, Zhang J. Chem Biol Interact. 2014 Aug 5;219:57-63. Doi: 10.1016/J.Cbi.2014.05.009. Epub 2014 May 28. Sophoricoside Fails The Embryo Implantation By Compromising The Uterine Endometrial Receptivity At Impla...
Lyu Z, Mao Z, Wang H, Fang Y, Chen T, Wan Q, Wang M, Wang N, Xiao J, Wei H, Li X, Liu Y, Zhou Q. Biochem Biophys Res Commun. 2013 Nov 1;440(4):495-501. Doi: 10.1016/J.Bbrc.2013.09.059. Epub 2013 Sep 20. Mir-181B Targets Six2 And Inhibits The Proli...
Huang W, Lu C, Wu Y, Ouyang S, Chen Y. Biochem Biophys Res Commun. 2015 Mar 6;458(2):294-9. Doi: 10.1016/J.Bbrc.2015.01.103. Epub 2015 Jan 31. Identification And Functional Characterization Of Voltage-Gated Sodium Channels In Lymphocytes.

FAQs

Q: Can I use this buffer for tissue lysate preparation in ELISA? Keywords: protocol, alternative buffer, applications
A: Yes, RIPA lysis buffer can be used for tissue lysate preparation in ELISA.
Q: What is the RIPA Lysis Buffer (SKU: AR0105 ) composed of? Keyword: ingredients, composition
A: It is composed of SDS, NP-40, Sodium deoxycholate and 0.02% Sodium azide.