Custom Antibody Development that works for you

Custom antibody production service

Need an antibody for your application? Use Boster Bio's battle-proven antibody discovery systems to make antibodies that work for you.

Since 1993, we have:

1. Designed and produced 40,000+ antibodies

2. Been cited in 30,000+ research publications

3. Contributed to many diagnostic and clinical projects

4. Earned recognition as one of the largest antibody manufacturers

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POPULAR CUSTOM ANTIBODY SERVICES

Click one of the following service to see details.

Custom Rabbit Polyclonal
Antibody

Boster offers custom polyclonal antibody generation service starting at $1300. Great for WB, IP, IHC

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Custom Mouse Monoclonal
Antibody

Boster offers custom hybridoma generation service starting at $4,000. Great for IHC, IF, Flow, WB, IP, MassSpec

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$600 Polyclonal
Promotion

For only $600! This is the big hit among scientists who use zebrafish, drosophila, yeast, plants, and anything other than human, mouse or rat.

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Custom Recombinant Rabbit Monoclonal Antibody

Our proprietary technology screens for plasma B cells only, which isolates candidate clones with the highest affinities right from the start.

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Custom Antibody Service overview

You need reliable antibodies for your assays. There might not be commercially available antibodies that work for you. Worry not, because Boster Bio has systems in place to make you the antibodies you need, fast, whether they are

1. low cost research-use-only (RUO) antibodies or

2. the critical reagents in your diagnostic assays (DX), or

3. the therapeutic candidates you will develop into drugs

The antibody experts at Boster Bio have helped our clients develop all of the above successfully, since 1993, and we have a variety of antibody discovery platforms to fit your unique needs:

1. rabbit polyclonal--low cost, RUO.
PS: We have an amazing $600 promotion (75%+ off) for polyclonal, check it out

2. mouse monoclonal--affordable, RUO and DX

3. recombinant rabbit monoclonal--advanced, supreme quality for DX and therapeutics

From antigen design to antibody scale up production, our antibody experts have you covered. Take advantage of our free consultations and discuss anything you want to. Below are some reasons for choosing Boster Bio for your custom antibody production:

PhD-level support
great communication

Experience
since 1993

30,000+
publications

Proprietary
technologies

40,000+
antibodies made

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ready to decide ?

If you already decided that you want to get some free consultations from us, click the button above and get started! And if not...

We understand that it takes time to research and evaluate your options. To make it easy for you, our antibody experts have curated a guide covering the important things to consider when deciding on antibody production methods/vendors. We have also collected the most common options for antibody discovery and compared these options for your convenience. We care about your success, and we are happy to give you personalized advices for free, book a consultation call with us, so our antibody production experts can sort through your unique needs with you.

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5 things to consider for ANTIBODY production

When it comes to making antibodies, think about these decisions. Ask us anything.

  • 1. Designing Antigen

    If your biomarker is a protein or peptide, there are 4 common ways to design antigens for it. You can make a peptide or recombinant protein that reflects the epitope(s) you want the antibody to specifically bind to. Or you can immunize with full length proteins or whole cell/tissue lysates, and screen for the clones that are specific to your biomarker(s)--this is for monoclonal only. In general, recombinant protein is the best option as it beats peptide on high immunogenicity, and beats whole protein or cell/tissue on specificity.

  • 2. deciding the discovery platform

    There are 4 common platforms for antibody discovery. Polyclonal, hybridoma monoclonal, in vivo recombinant (such as our rabbit monoclonal platform), and phage display.

    Cost: polyclonal < hybridoma monoclonal < rabbit recombinant monoclonal = phage display

    Application: polyclonal and hybridoma monoclonal are good for RUO, while recombinant monoclonal and phage display are good for DX and therapeutic applications.

    If you want antibodies for difficult biomarkers, or for diagnostics or therapeutics, go with the more expensive platforms. If your biomarkers are easy to make antibodies against and you want a fast turnaround time, use the low cost platforms.

  • 3. Choosing the host

    Common hosts for antibody production are rabbit and mouse.

    In 1980s, scientists including Georges Köhler and César Milstein pioneered the first generation of monoclonal technology, using mouse. Today, many scientists can make mouse monoclonal antibodies.

    Rabbit has a better immune system and can generate higher affinity antibodies. Rabbit monoclonal antibodies have earned a reputation for themselves in the past decade and are sought after by researchers and assay developers.

    In case of multiplexing staining, you will need antibodies from different hosts to make distinct stains of your biomarkers, and you might need to explore more exotic hosts such as donkeys, guinea pigs, goats, llamas, and sometimes, sharks (dododo, dodododo...).

    Click to read a more in-depth article about benefits of rabbit monoclonal.

  • 4. Using The Right Adjuvant

    If you are using a platform that involves immunization (i.e. anything other than phage display), you might run into the problem of low immune response from the host. Most of the time the class Freund's Adjuvant is sufficient in eliciting immune response but we have run into situations where a custom adjuvant was the solution to a difficult to make antibody. If you have exhausted everything else, look into adjuvants and you might find something.

  • 5. Selecting the vendors

    While the principle of antibody development is not beyond comprehension, there are many uncertainties that go into the process. A lot of these uncertainties can be mitigated with expertise and experience. When selecting vendors, pick the ones with most experience with your particular application is key to success.
    Communication is also important, so in your initial contacts with potential vendors, look for the ones who exhibit a customer-service centric culture and good communication habits, because if something goes awry, and more than likely something will, you want a responsive point of contact to be there for you and solve the problems with you.

Antibody production
decision Guide

Click to enlarge

Compare Your options

Here are some common options for custom antibody production services. We organized their pros and cons for you. Choose an option to see details

Boster Solutions

Polyclonal for rare species

Standard rabbit polyclonal at only $600. Only for rare species (not human or murine) targets.

Cost - lowest in market
Turnaround ++ 3+ months
Specificity ++ good
Repeatability ++ good
Scenarios
Research using model organisms that are hard to find antibodies for (not human or murine)

Polyclonal by various hosts

We can make your antibody in goat, chicken, guinea pig, etc. Great for multiplex staining.

Cost + low
Turnaround ++ 3+ months
Specificity ++ good
Repeatability ++ good
Scenarios
Research use only. Great for multiplex IHC/IF staining

Mono hybridoma

The classic monoclonal method, great for research and diagnostic use.

Cost ++ high
Turnaround ++ 6+ months
Specificity +++ very good
Repeatability +++ very good
Scenarios
Diagnostics and Research

Rabbit recombinant

State of art recombinant rabbit monoclonal platform powered by our PCD technology.

Cost +++ quite high
Turnaround ++ 6+ months
Specificity ++++ best there is
Repeatability +++ very good
Scenarios
Diagnostics, theraputics (easy for humanization)
vs.

Workarounds

do it in-house

Generate antibodies in-house.

Cost ??
Turnaround ??
Specificity ??
Repeatability ??
Scenarios
Unless you have strong reasons for it, generating antibody in-house is not recommended.

Knock-in tag

Knock in a fluorescent tag to your protein of interest.

Cost ++ high
Turnaround + a few weeks
Specificity ++++ best there is
Repeatability ++++ best there is
Scenarios
Only works well if you have a fast reproducing model organism and the skills. The applications are also limited

In situ hybridization/qPCR

Give up on protein level evidence and go for mRNA instead.

Cost + low
Turnaround + a few weeks
Specificity - not ideal
Repeatability + OK
Scenarios
For studies that only require mRNA level evidence and studies in exploratory stages.

Phage display

Screen phages with randomized CDRs against target of interest, then improve specificity via antibody engineering.

Cost++++very high
Turnaround+++
many months
Specificity ++good
Repeatability +++very good
Scenarios
Unless you have strong reasons for it, generating antibody in-house is not recommended.

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How to work with us ?

It is as easy as 1, 2, 3.

Provide us your antigen

3 options: give us 2mg+ of the antigen, or provide the exact sequence so we can synthesize it, or tell us the biomarker and we will design the antigen for you.

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We Produce your antibody

Over the next few months, we will carry out the antibody production for you turn-key style. You will receive regular updates from us about your project progress.

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Receive the antibody

Upon successful production, receive the antibody with a final QC report. Additional validations (WB, IHC, ELISA, Flow etc.) can be ordered.

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Example data & reports

A sneak peak to what you can expect from us.

Example results images

Sample report

FAQs

Frequently asked questions about custom antibody services.

Q1. Do I qualify for the $600 polyclonal package?

You have to either use a model organism that is not human or murine, or trying to replace a discontinued antibody from another company.

Q2. What are benefits of your recombinant rabbit monoclonal antibody?

Rabbit monoclonals have higher affinity compared to mouse monoclonals. Rabbits have more diverse CDR repitior which allows their immunesystems to come up with clones that more tightly bind to the antigen. They are great for theraputic and diagnostic antibodies.

Q3. What host should I choose?

If you are doing double staining you could use our "exotic host" package to generate antibodies from goat, chicken, guinea pig, and various other hosts. For other situations, check out our decision guide infographic (on this page) for more information.

Q4. Do you validate the custom antibodies?

We provide very affordable validation services at additional costs. We can perform WB, IHC/IF/ICC, ELISA, Flow and various other assays if we can obtain the samples. The cost is a fraction of what it would cost for you to do it in house and our customers find this option attractive.

Q5. How much antibodies do I get?

With the default pacakge, you will receive 100ug with the $600 promotional pacakge, and 1-10 mg from other packages. See individual pages for details.