Multiplex Cytokine Assay Service

Samples

Requirements:

Sample Types – The samples can be serum, plasma, cell culture supernatant, cell lysate, cell culture media, and/or tissue homogenates. Please provide a list of the samples in the shipment.
Sample Volume – It is optimal for at least 200 μl of each sample to be sent per array. This volume allows for triplicate testing of each sample.
Minimum number of 8 samples per array is required.

Sample Types: We test a wide variety of sample types and species from bioresearch. The most common samples customers send us are: serum, plasma, cell culture supernates, cell lysates, and tissue homogenates.

Common Sample Species:

Human
Mouse
Rat
Primate (non-human)
Cat
Pig
Rabbit

Common Sample Types

Cell Culture

Cell Culture Media
Conditioned Media
Media + Sera
Cell Culture Supernatants
Cell Culture Lysates

Tissue

Tissue Homogenates
Biopsy Tissues
Tumors
Eye
Liver

Fluids

Blood; Plasma/Sera
Bronchoalveolar Lavage
Aqueous Humor
Peritoneal Fluid
Urine

Assays: Our immunoassay sample testing service allows for measurement of multiple protein biomarkers. We offer an extensive targeted menu of assays performed on our multiplex ELISA array platform and applicable single ELISAs. The list below contains the most common analytes we’re asked to test for.

Validated Human Analytes

Adiponectin	GCSF	IL-1Ra	IL-27	PDGF
Adipsin	Ghrelin	IL-2	IL-33	PF-4
AGP	Glucagon	IL-4	IP-10	P-Selectin
Ang-2	GMCSF	IL-5	Leptin	RANTES
poly-His	GROα	IL-6	MCP-1	RBP4
CD-14	HCGβ	IL-6R	MCP-2	Resistin
CD-26	HGF	IL-7	MCP-3	S100A9
CD-163	HRP2	IL-8	MIG	sFAS
Cortisol	I-309	IL-10	MIP-1α	sTfR
C-Peptide	IFNα	IL-12p40	MIP-1β	TARC
CRP	IFNβ	IL-12p70	MMP 1	Tg
CTACK	IFNλ	IL-13	MMP 2	TGFβ
CXCL-5	IFNω	IL-15	MMP 3	TIMP-1
E1G	IFNγ	IL-16	MMP 7	TIMP-2
E2	IL-1α	IL-17	MMP 9	TNFα
Eotaxin	IL-1β	IL-18	MMP 13	TNFβ
Ferritin	IL-1R1	IL-18BPa	MPO	TNFRI
FGF	IL-1R2	IL-21	P4	TNFRII
Fractalkine	IL-1R3	IL-22	PAI-1	VEGF
FSH	IL-1R4	IL-23	PDG

Validated Mouse Analytes

Eotaxin	IL-1α	IL-5	IL-17	MIP-2
GMCSF	IL-1β	IL-6	KC	RANTES
IFNα	IL-2	IL-10	MCP-1	TARC
IFNβ	IL-3	IL-12	MDC	TCA-3
IFNγ	IL-4	IL-13	MIP-1α	TNFα

Validated Rat Analytes

IFNγ	IL-1β	IL-4	IL-10	TNFα
IL-1α	IL-2	IL-6	IL-12p70

Validated Porcine Analytes

IL-1β

IL-6

IL-8

TNFα

Process

We appreciate the trust you place in us, that’s why we emphasize quality in everything we do. We’ll implement the following measures to ensure you’re satisfied with our results.

Samples are handled with care and respect to ensure continuity from your lab to ours.
Validated and time proven protocols are meticulously followed from sample receipt to report generation to ensure reproducibility.
All assays are prepared by highly experienced technicians, so you can be confident in the results.
All samples are run in replicate and all results are thoroughly evaluated for reproducibility and reliability.
Results are obtained using highly sensitive validated kits and delivered to you in a detailed report.

Results

All results will be reported in a Microsoft Excel spreadsheet format. The spreadsheet will contain raw data, charts, and calculated concentrations derived from regression models from each of the samples tested. A completed report of the samples will be emailed to the customer typically within 2 weeks (depending on the assays requested). To request a quote, contact us at support@bosterbio.com today!