Goat Anti-Mouse IgG (H+L) Secondary Antibody, TRITC Conjugated

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Cited in 10 publication(s).

Product Info Summary

SKU: BA1089
Size: 0.5ml
Reactive Species: Mouse
Host: Goat
Application: Flow Cytometry, IF

Product Overview

Product Name Goat Anti-Mouse IgG (H+L) Secondary Antibody, TRITC Conjugate
Synonyms TRITC-conjugated Goat Anti-Mouse IgG; Goat Anti-Mouse IgG-TRITC Secondary Antibody; Rhodamine-labeled Goat Anti-Mouse IgG Secondary Antibody
Description Goat Anti-Mouse IgG (H+L) Secondary Antibody, TRITC Conjugate, for detection, localization and quantification of target proteins in a sample via indirect immunofluorescence in IHC-P, IHC-F or ICC
Reagent Type Fluorophore-conjugated secondary antibody
Conjugate TRITC
Host Goat
Target Species Mouse
Antibody Class IgG
Clonality Polyclonal
Immunogen Whole molecule mouse IgG
Purification Immunoaffinity chromatography
Specificity Mouse IgG specific
Form Supplied Liquid: concentrated buffered stock solution
Formulation 0.5 mg TRITC-conjugated secondary antibody
0.01 M PBS (PH 7.4)
5mg/mL BSA
50% glycerol
Pack Size 0.5 ml
Concentration 1 mg/ml
Application IF, Flow Cytometry
Our Boster Guarantee covers the use of this product in the above marked tested applications.
Storage At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type Mouse primary-antibody-probed formalin-fixed paraffin-embedded (FFPE) tissue sections on slides (IHC-P), or thawed frozen samples (IHC-F)
Assay Type Immunoanalytical
Technique Indirect immunofluorescence
Assay Purpose Protein detection/quantification
Equipment Needed Excitation light source, filter set and detector, fluorescence microscope (can be combined with confocal microscope), fluorescence plate-reader, flow cytometer, or cell sorter

Main Advantages

Specific High signal-to-noise ratio
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody, multiple TRITC molecules bind to a single secondary antibody
Fast Fewer processing steps - no need to add a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; signal can be observed directly
Quantifiable The digital nature of the gold signal + high precision in allocating gold labels to defined structures makes it easy to count and quantify
Flexible No need to label each antibody against each target protein with a fluorescent dye, the small size of TRITC causes no steric interference with proper biological function of target proteins or antibodies
Multiplex Compatible Compatible with colocalization studies (multiple antigens concurrent detection) even in close proximity using primary antibodies from different host species for simultaneous detection by fluorophore-conjugated secondary antibodies, or using multiple differently colored fluorophores (FITC and TRITC) in the same experiment for target differentiation.

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and then modified with antibody fragmentation, label conjugation, etc., to generate highly specific detection reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, fluorescent dyes/proteins, or gold particles. Here, the antibody provides the specificity to locate the protein of interest by recognizing a primary antibody that targets a particular antigen, and the label generates a detectable signal in the area of the formed immune complexes. The label of choice depends upon the experimental application.

Fluorescent reporters widely used in biological research are of two types: organic compounds with a low molecular weight (0.2-1 kDa) typically containing numerous aromatic groups, or plane or cyclic moieties with π bonds (e.g.FITC, TRITC, Alexa Fluor Dyes, DyLight Fluors), and biological fluorophores (e.g.green fluorescent protein (GFP), R-Phycoerythrin). TRITC (tetramethylrhodamine isothiocyanate )is a bright orange-fluorescent dye. It is a derivative of rhodamine (a member of the fluorone dyes family) where a hydrogen atom on the bottom ring of the structure is replaced by isothiocyanate functional group (-N=C=S), making it more reactive to amine and sulfhydryl groups on proteins. The bond to antibodies is based on this reactive group. The excitation and emission wavelengths of TRITC are 550 nm and 573 nm respectively. The optimal degree of conjugation for least changes in the antibody affinity and maximal specific staining (fluorescence) is at a molecular ratio TRITC/Ab of approximately 2. Like most fluorochromes, TRITC is prone to photobleaching, i.e. losing fluorescing properties due to molecule structure degradation. TRITC is inherently a quite stable dye, and thus exhibits less photobleaching than fluorescein, which is commonly used as a photobleaching standard.Loss of activity caused by photobleaching can be controlled by reducing the intensity or time-span of light exposure, by increasing the concentration of the fluorophore, or by employing more robust fluorophores that are less prone to bleaching (e.g., Alexa Fluors, Seta Fluors, or DyLight Fluors). Analogs of TRITC with greater photostability and higher fluorescence intensity tailored in various biological applications are Alexa 555 and DyLight 550.

Validation Images & Assay Conditions

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BA1089 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Spred2 inhibits epithelial‑mesenchymal transition of colorectal cancer cells by impairing ERK signaling

Taurolidine promotes cell apoptosis by enhancing GRIM‑19 expression in liver cancer

2-Methoxyestradiol inhibits the proliferation and migration and reduces the radioresistance of nasopharyngeal carcinoma CNE-2 stem cells via NF-κB/HIF-1 signaling pathway inactivation and EMT reversal

Effects of valproic acid on the susceptibility of human glioma stem cells for TMZ and ACNU

Munc13‑4 mediates human neutrophil elastase‑induced airway mucin5AC hypersecretion by interacting with syntaxin2

Resveratrol alleviates sepsis‑induced myocardial injury in rats by suppressing neutrophil accumulation, the induction of TNF‑α and myocardial apoptosis via activation of Sirt1

Localization and expression of heat shock protein 70 with rat myocardial cell damage induced by heat stress in vitro and in vivo

SOX9 overexpression plays a potential role in idiopathic congenital talipes equinovarus

Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin‑angiotensin system

Identification of Telocytes in the Pancreas of Turtles—A role in Cellular Communication

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