Rabbit Anti-Mouse IgG (H+L) Secondary Antibody, Unconjugated

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all rabbit serum proteins, except the specific antibody for mouse IgG. Cited in 20 publication(s).

Product Info Summary

SKU: BA1048
Size: 1mg
Reactive Species: Mouse
Host: Rabbit

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Product NameAffiniPure Rabbit Anti-mouse IgG (H+L)
SKU/Catalog NumberBA1048
Ig TypeIgG
Pack Size1 mg
Formlyophilized
ImmunogenMouse IgG (whole molecular).
PurityThe antibody was purified from antiserum by immunoaffinity chromatography..
HostRabbit
ClonePolyclonal
Contents1 mg of affinity purified specific antibody (purification>99%). 0.01M PBS.
Usage MethodRecommended Dilutions: Optimal working dilutions must be determined by end users. Working solution can not be preserved for long after being diluted with 0.01M PBS buffer solution (pH7.0-7.6) or 1% sodium acetate unless adding antiseptic as 0.05% NaN3 and BSA to 1%.
Cite This ProductRabbit Anti-mouse IgG (H+L) (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1048)

Validation Images & Assay Conditions

Hello CJ!

BA1048 has been cited in 20 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Suppression of wingless‑type MMTV integration site family, member 1 expression by small interfering RNA inhibits U251 glioma cell growth in vitro

Trimetazidine improves hepatic lipogenesis and steatosis in non‑alcoholic fatty liver disease via AMPK‑ChREBP pathway

Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation

PINK1/Parkin-Mediated Mitophagy Regulation by Reactive Oxygen Species Alleviates Rocaglamide A-Induced Apoptosis in Pancreatic Cancer Cells

miR-138-5p suppresses autophagy in pancreatic cancer by targeting SIRT1

rhIL-1Ra reduces hepatocellular apoptosis in mice with acetaminophen-induced acute liver failure

Astragaloside IV Regulates Expression of ATP-sensitive Potassium Channel Subunits after Ischemia-reperfusion in Rat Ventricular Cardiomyocytes

Neuronal zinc-α2-glycoprotein is decreased in temporal lobe epilepsy in patients and rats

Role of Rho-mediated ROCK-Semaphorin3A signaling pathway in the pathogenesis of Parkinsons disease in a mouse model

Combination of Ligusticum chuanxiong and Radix Paeoniae ameliorate focal cerebral ischemic in MCAO rats via endoplasmic reticulum stress-dependent apoptotic signaling pathway

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3 Customer Q&As for Rabbit Anti-Mouse IgG (H+L) Secondary Antibody, Unconjugated

Question

If BA1048 is lyophilized then itt contains affinity purified antibodies. The Buffer is described as 0.01M PBS, 0.01% Thimerosal. Is this the buffer before lyophilization? Do we then only need to add sterile water for reconstitution? We would like to know what volume needs to be added to restore the buffer before lyophilization (0.01M PBS, 0.01% Thimerosal), if we receive 5mg vial of #ba1048-5. Is it 5ml? Was the concentration 1mg/ml?

Verified customer

Asked: 2022-06-14

Answer

For the Rabbit Anti-Mouse IgG (H+L) Secondary Antibody, Unconjugated (BA1048), the Buffer is described as 0.01M PBS, 0.01% Thimerosal. This is the buffer before lyophilization. 400ul of distilled water needs to be added into 5mg vial to restore the buffer before lyophilization (0.01M PBS, 0.01% Thimerosal).

Boster Scientific Support

Answered: 2022-06-15

Question

Could you please let us know the volume of 0.01M PBS buffer that is recommended for reconstitution of BA1048?

Verified customer

Asked: 2022-06-09

Answer

The volume of 0.01M PBS buffer that is recommended for reconstitution of Rabbit Anti-Mouse IgG (H+L) Secondary Antibody, Unconjugated (BA1048) depends on the conjugation type (Biotin, HRP, FITC) and application of the antibody (IHC/IC/ELISA, WB/ELISA, IF/ELISA).

Boster Scientific Support

Answered: 2022-06-10

Question

Could you please let us know how to reconstitute BA1048?

Verified customer

Asked: 2022-06-07

Answer

For the Rabbit Anti-Mouse IgG (H+L) Secondary Antibody, Unconjugated (BA1048), please see the following Usage Method for reconstitution. Usage Method Recommended Dilutions: Optimal working dilutions must be determined by end users. Working solution can not be preserved for long after being diluted with 0.01M PBS buffer solution (pH7.0-7.6) or 1% sodium acetate unless adding antiseptic as 0.05% NaN3 and BSA to 1%. Reconstitute the antibody with 0.01M PBS buffer solution (pH7.0-7.6) or 1% sodium acetate.

Boster Scientific Support

Answered: 2022-06-08

Size

Total: $50

SKU:BA1048

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