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Product Info Summary
| SKU: | M01687 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-14-3-3 epsilon YWHAE Rabbit Monoclonal Antibody
SKU/Catalog Number
M01687
BM4755 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-14-3-3 epsilon YWHAE Rabbit Monoclonal Antibody catalog # M01687. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 epsilon YWHAE Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01687)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HHD-25
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human 14-3-3 epsilon
Reactive Species
M01687 is reactive to YWHAE in Human, Mouse, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
29.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01687 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of 14-3-3 epsilon expression in 293T cell lysate.
Specific Publications For Anti-14-3-3 epsilon YWHAE Rabbit Monoclonal Antibody (M01687)
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Customer Q&As
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2 Customer Q&As for Anti-14-3-3 epsilon YWHAE Rabbit Monoclonal Antibody
Question
Is this M01687 anti-14-3-3 epsilon Rabbit Monoclonal antibody reactive to the isotypes of YWHAE?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
The immunogen of M01687 anti-14-3-3 epsilon Rabbit Monoclonal antibody is A synthesized peptide derived from human 14-3-3 epsilon . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-23
Question
We are currently using anti-14-3-3 epsilon Rabbit Monoclonal antibody M01687 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
K. Baker
Verified customer
Asked: 2014-08-01
Answer
The anti-14-3-3 epsilon Rabbit Monoclonal antibody (M01687) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-08-01
