|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-5HT2A Receptor Antibody|
|Description||Rabbit IgG polyclonal antibody for 5-hydroxytryptamine receptor 2A(HTR2A) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-5HT2A Receptor Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1373)|
|Replacement Item||This antibody may replace the following items: sc-15073|sc-15074|sc-166775|sc-32537|sc-32538|sc-50396 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT2A Receptor(418-432aa AYKSSQLQMGQKKNS), different from the mouse sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||5-hydroxytryptamine receptor 2A(5-HT-2/5-HT-2A)|
|Molecular Weight||52603 MW|
|Protein Function||G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5- dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca(2+) ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction. .|
|Tissue Specificity||Detected in brain cortex (at protein level). Detected in blood platelets. .|
|Sequence Similarities||Belongs to the G-protein coupled receptor 1 family.|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein. Cell projection, dendrite . Cell projection, axon . Cytoplasmic vesicle . Membrane, caveola . Localizes to the postsynaptic thickening of axo-dendritic synapses. .|
|Alternative Names||5-hydroxytryptamine receptor 2A;5-HT-2;5-HT-2A;Serotonin receptor 2A;HTR2A;HTR2;|
|Research Areas|||neuroscience|neurotransmission|receptors / channels|gpcr|serotonin receptors| metabolism|types of disease|obesity||
Background for 5-hydroxytryptamine receptor 2A(5-HT-2/5-HT-2A)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-5HT2A Receptor Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-5HT2A Receptor Antibody Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
Lane 5: U87 Cell Lysate
Lane 6: SMMC Cell Lysate
Lane 7: HT1080 Cell Lysate
Lane 8: COLO320 Cell Lysate
IHC(P): Rat Brain Tissue
- Review by Saikat Chakraborty
Source: Customer Feedback Submission
"It works far better compared to other vendors"
--Saikat Chakraborty, PhD, Feinberg School of Medicine, Northwestern University
Applications Western Blot Sample Mouse brain lysate Detection Licor Imaging and ImageJ
"We have tested PA1373 trial sample in our lab and it works far better compared to other vendors. We are extremely happy to get the results. We looked for 5HT2A expression in various brain regions. Our bands show clear expression level of 5HT2A. We now plan to buy this antibody for all our laboratory needs."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,