|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-ACTH Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Pro-opiomelanocortin(POMC) detection. Tested with IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-ACTH Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9039)|
|Replacement Item||This antibody may replace the following items: sc-20148|sc-18263 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human ACTH(138-176aa SYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF), different from the related mouse and rat sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||29424 MW|
|Protein Function||ACTH stimulates the adrenal glands to release cortisol.|
|Tissue Specificity||ACTH and MSH are produced by the pituitary gland.|
|Sequence Similarities||Belongs to the POMC family.|
|Alternative Names||Pro-opiomelanocortin;POMC;Corticotropin-lipotropin;NPP;Melanotropin gamma;Gamma-MSH;Potential peptide;Corticotropin;Adrenocorticotropic hormone;ACTH;Melanotropin alpha;Alpha-MSH;Corticotropin-like intermediary peptide;CLIP;Lipotropin beta;Beta-LPH;Lipotropin gamma;Gamma-LPH;Melanotropin beta;Beta-MSH;Beta-endorphin;Met-enkephalin;POMC;|
|Research Areas|||neuroscience|neurotransmitter|neuropeptides|hormones| signal transduction|growth factors/hormones|metabolism|energy metabolism| neuroscience|neurology process|endocrine system|hypothalamic pituitary adrenal axis| cancer|tumor biomarkers|cancer metabolism|metabolic signaling pathway|hormone biosynthesis| metabolism|pathways and processes|endocrine metabolism|types of disease||
Background for Pro-opiomelanocortin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ACTH Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ACTH Picoband™ Antibody Images
Click the images to enlarge.
IHC(P): Mouse Kidney Tissue
IHC(P): Rat Brain Tissue
IHC(P): Rat Kidney Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,