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Product Info Summary
| SKU: | M01005 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-Htr2a Antibody (N-term)
SKU/Catalog Number
M01005
Size
80 µl
Description
Boster Bio Anti-Htr2a Antibody (N-term) (Catalog # M01005). Tested in WB, IHC-P application(s). This antibody reacts with Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Htr2a Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01005)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Htr2a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-30 amino acids from the N-terminal region of human Htr2a.
Reactive Species
M01005 is reactive to Htr2a in Mouse
Calculated molecular weight
52.8 kDa
Background of Htr2a
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5- dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca(2+) ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01005 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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Anti-Htr2a Antibody (N-term). ctrlat 1:2000 dilution + mouse heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 53 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M01005 staining Htr2a in mouse skeletal muscle sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-Htr2a Antibody (N-term) (M01005)
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