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Anti-SLC10A1/NTCP1 Antibody Picoband™
Boster Bio Anti-SLC10A1/NTCP1 Antibody Picoband™ catalog # A06872. Tested in WB applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SLC10A1/NTCP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06872)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence of mouse SLC10A1/NTCP1 (DQTKITYKAAATEDATPAALEK).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A06872 is reactive to Slc10a1 in Rat
A06872 is guaranteed for WB Boster Guarantee
Background of Slc10a1
Sodium/bile acid cotransporter also known as the Na+-taurocholate cotransporting polypeptide (NTCP) or liver bile acid transporter (LBAT) is a protein that in humans is encoded by the SLC10A1 (solute carrier family 10 member 1) gene. It is mapped to 12 C3-D1; 12 37.21 cM. The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC10A1/NTCP1 using anti-SLC10A1/NTCP1antibody (A06872).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1/NTCP1 antigen affinity purified polyclonal antibody (Catalog # A06872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1/NTCP1 at approximately 50KD. The expected band size for SLC10A1/NTCP1 is at 50KD.
Gene/Protein Information For Slc10a1 (Source: Uniprot.Org, NCBI)
Sodium/bile acid cotransporter
bile acid:sodium symporter (BASS) (TC 2.A.28) family
Cell growth-inhibiting gene 29 protein; Na(+)/bile acid cotransporter; Na(+)/taurocholate transport protein; Na/taurocholate cotransporting polypeptide; NTCP; NTCP1; NTCPgrowth-inhibiting protein 29; sodium/bile acid cotransporter; sodium/taurocholate cotransporter; Sodium/taurocholate cotransporting polypeptide; solute carrier family 10 (sodium/bile acid cotransporter family), member 1; Solute carrier family 10 member 1 Slc10a1|Nt, Ntcp|solute carrier family 10 (sodium/bile acid cotransporter family), member 1|sodium/bile acid cotransporter|Na(+)/bile acid cotransporter|Na(+)/taurocholate transport protein|bile acid cotransporting polypeptide|sodium bile acid cotransporting polypeptide|sodium-taurocholate cotransporting polypeptide*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Slc10a1, check out the Slc10a1 Infographic
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In this infographic you will see the following information for Slc10a1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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