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- Table of Contents
ABCB11 antibody (PB9414) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
BSEP/ABCB11 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BSEP/ABCB11 Antibody (PB9414) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
BSEP/ABCB11 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BSEP/ABCB11 Antibody (PB9414) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-ABCB11 Antibody Picoband®
ABCB11 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, WB |
Tested Applications
ABCB11 antibody was positively detected by WB in:
human HCCT tissue, rat liver tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ABCB11 antibody was positively detected by IHC in:
Mouse Liver tissue, Rat Liver tissue, Human Liver Cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ABCB11 antibody was positively detected by IF in:
mouse liver tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunofluorescence
2μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Tested
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-ABCB11 Antibody Picoband®
View all ABCB11 Antibodies
View all ABCB11 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB11, different from the related mouse and rat sequences by three amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
146 kDa
Calculated Molecular Weight
146.4 kDa
Background of ABCB11
Bile Salt Export Pump (BSEP) is a protein which in humans is encoded by the ABCB 11 gene. It is a member of the superfamily of ATP-binding cassette (ABC) transporters, also known as ABCB11. It is mapped to chromosome 2q24. The BSEP protein is mainly expressed in the liver. ABCB11 is a gene associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) which caused by mutations in the ABCB11 gene will increases the risk of hepatocellular carcinoma in early life.
Cite This Product
Anti-ABCB11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9414)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
96%
High Homology
Rabbit
92%
High Homology
Cow
92%
High Homology
Sheep
92%
High Homology
Mouse
88%
Moderate Homology
Rat
88%
Moderate Homology
Dog
88%
Moderate Homology
Zebrafish
64%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ABCB11 antibody
Immunohistochemistry (IHC) protocol for ABCB11 antibody
Immunofluorescence (IF) protocol for ABCB11 antibody
Publications
Application:
Species:
Lactobacillus johnsonii mediates the protective effects of pristimerin against ulcerative colitis and concomitant liver injury through remodeling hepatic lipid metabolism via LXRα–SCD1 axis
Published: 2026-07-17
Journal: Gut Microbes
Application: WB
Impact Factor: 15.30
Species: Mouse
PMID: 42470108
Emodin and physcion alleviate cholestatic liver injury by targeting FXR: hepatoprotective components identified in processed Polygonum multiflorum Thunb. using a comprehensive two-dimensional biochromatography system
Published: 2025-12-19
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 41487497
Generation and characterization of a mouse model for bile salt export pump deficiency with the p.E297G mutation
Published: 2025-10-17
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Application: WB,IF
Impact Factor: 3.30
Species: Mouse
PMID: 41110689
Geniposide induces hepatotoxicity via the bile acid-induced activation of NLRP3 inflammasome and regulation of the FXR/PERK/TXNIP pathway
Published: 2025-10-01
Journal: TOXICOLOGY LETTERS
Application: WB
Impact Factor: 2.90
Species: Rat
PMID: 41043658
Female ethanolamine phosphate phospholyase knockout mice resisted high-fat diet-induced obesity with attenuated hepatic cholesterol deposition
Published: 2025-07-18
Journal: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
Application: WB
Impact Factor: 3.30
Species: Mouse
PMID: 40679478
Achyranthes bidentata polysaccharides alleviate alcohol-induced gut-liver injury via Farnesoid X Receptor pathway
Published: 2025-05-02
Journal: Food Bioscience
Application: WB
Impact Factor: 5.90
Species: Mouse
A liver-specific mouse model for MYO5B-associated cholestasis reveals a toxic gain-of-function as underlying disease mechanism
Published: 2025-03-20
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: IF
Impact Factor: 2.20
Species: Mouse
PMID: 40127562
Loss of hepatocyte Usp53 protects mice from a form of xenobiotic-induced liver injury
Published: 2024-12-19
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Application: IF
Impact Factor: 4.20
Species: Mouse
PMID: 39705897
Molecular alterations associated with pathophysiology in liver-specific ZO-1 and ZO-2 knockout mice
Published: 2024-10-26
Journal: CELL STRUCTURE AND FUNCTION
Application: WB,IF
Impact Factor: 2.00
Species: Mouse
PMID: 39322562
Hypercholesterolemia risk associated Abca6 does not regulate lipoprotein metabolism in mice or hamster
Published: 2021-07-16
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Application: WB
Impact Factor: 4.70
Species: Mouse
PMID: 34274505
The zonula occludens protein family regulates the hepatic barrier system in the murine liver
Published: 2020-10-22
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Application: WB
Impact Factor: 4.35
Species: Mouse
PMID: 33184034
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Product Promise
We promise Anti-ABCB11 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9414
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026