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ABI2 antibody (PA2068) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of SH-SY5Y cells using anti-ABI2 antibody (PA2068).
Overlay histogram showing SH-SY5Y cells stained with PA2068 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABI2 Antibody (PA2068, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SH-SY5Y cells stained with PA2068 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABI2 Antibody (PA2068, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Abl interactor 2 ABI2 Antibody Picoband®
ABI2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, ICC, WB |
Tested Applications
ABI2 antibody was positively detected by WB in:
human SH-SY5Y whole cell, human 293T whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ABI2 antibody was positively detected by ICC/IF in:
A549 cell
ABI2 antibody was positively detected by FC in:
SH-SY5Y cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-Abl interactor 2 ABI2 Antibody Picoband®
View all ABI2 Antibodies
View all ABI2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ABI2, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56-70 kDa
Calculated Molecular Weight
55.7 kDa
Background of ABI2
ABI2 (ABL Interactor 2), is a protein that in humans is encoded by the ABI2 gene. By analysis of a YAC and a BAC, Machado et al. (2000) mapped the ABI2 gene to 2q31-q33. ABI2 possesses a basic N terminus with homology to a homeodomain protein; a central serine-rich region; 3 PEST sequences, which are implicated in susceptibility to protein degradation; several proline-rich stretches; and an acidic C terminus with multiple phosphorylation sites and an SH3 domain. Dai and Pendergast (1995) suggested that the ABI proteins may function to coordinate the cytoplasmic and nuclear functions of the ABL1 tyrosine kinase.
Cite This Product
Anti-Abl interactor 2 ABI2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2068)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ABI2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ABI2 antibody
Flow Cytometry (FC) protocol for ABI2 antibody
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Product Promise
We promise Anti-Abl interactor 2 ABI2 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2068
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026