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Product Info Summary
| SKU: | A02419-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Alpha 1 microglobulin/AMBP Antibody Picoband®
SKU/Catalog Number
A02419-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha 1 microglobulin/AMBP Antibody Picoband® catalog # A02419-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha 1 microglobulin/AMBP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02419-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Alpha 1 microglobulin recombinant protein (Position: G20-V203).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02419-3 is reactive to AMBP in Human
Observed Molecular Weight
45 kDa
Calculated molecular weight
39.0 kDa
Background of AMBP
Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02419-3 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue, human HEP-2 whole cell, human Hela whole cell, human A549 whole cell, human PANC-1 whole cell
IHC: human liver cancer tissue, human liver cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Alpha 1 microglobulin using anti-Alpha 1 microglobulin antibody (A02419-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HEP-2 whole cell lysate,
Lane 3: human Hela whole cell lysate,
Lane 4: human A549 whole cell lysate,
Lane 5: human PANC-1 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 microglobulin antigen affinity purified polyclonal antibody (Catalog # A02419-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 microglobulin at approximately 45KD. The expected band size for Alpha 1 microglobulin is at 39KD.
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IHC analysis of Alpha 1 microglobulin using anti-Alpha 1 microglobulin antibody (A02419-3).
Alpha 1 microglobulin was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha 1 microglobulin Antibody (A02419-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of Alpha 1 microglobulin using anti-Alpha 1 microglobulin antibody (A02419-3).
Alpha 1 microglobulin was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha 1 microglobulin Antibody (A02419-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Alpha 1 microglobulin/AMBP Antibody Picoband® (A02419-3)
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5 Customer Q&As for Anti-Alpha 1 microglobulin/AMBP Antibody Picoband®
Question
I was wanting to use your anti-Alpha 1 microglobulin/AMBP antibody for WB for human plasma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human plasma identification?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
You can see on the product datasheet, A02419-3 anti-Alpha 1 microglobulin/AMBP antibody has been tested for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-16
Question
Can you help my question with product A02419-3, anti-Alpha 1 microglobulin/AMBP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02419-3 anti-Alpha 1 microglobulin/AMBP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-18
Question
Does anti-Alpha 1 microglobulin/AMBP antibody A02419-3 work for WB with plasma?
Verified Customer
Verified customer
Asked: 2018-03-05
Answer
According to the expression profile of plasma, AMBP is highly expressed in plasma. So, it is likely that anti-Alpha 1 microglobulin/AMBP antibody A02419-3 will work for WB with plasma.
Boster Scientific Support
Answered: 2018-03-05
Question
We are currently using anti-Alpha 1 microglobulin/AMBP antibody A02419-3 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
The anti-Alpha 1 microglobulin/AMBP antibody (A02419-3) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Alpha 1 microglobulin/AMBP antibody A02419-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-04
