Anti-ACE1 Monoclonal Antibody

ACE/CD143 antibody

Boster Bio Anti-ACE1 Monoclonal Antibody catalog # M00251. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse. Cited in 1 publication(s).

Product Info Summary

SKU: M00251
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IHC, WB

Product Name

Anti-ACE1 Monoclonal Antibody

View all ACE/CD143 Antibodies

SKU/Catalog Number

M00251

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ACE1 Monoclonal Antibody catalog # M00251. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ACE1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00251)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADCO-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ACE1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00251 is reactive to ACE in Human, Mouse

Applications

M00251 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

149.715kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:200
FC 1:30

Validation Images & Assay Conditions

Gene/Protein Information For ACE (Source: Uniprot.org, NCBI)

Gene Name

ACE

Full Name

Angiotensin-converting enzyme

Weight

149.715kDa

Superfamily

peptidase M2 family

Alternative Names

ACE; ACE1angiotensin converting enzyme, somatic isoform; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; carboxycathepsin; CD143 antigen; CD143; DCP; DCP1; DCP1angiotensin-converting enzyme; dipeptidyl carboxypeptidase 1; Dipeptidyl carboxypeptidase I; EC 3.2.1.-; EC 3.4.15.1; Kininase II; MGC26566; MVCD3; peptidase P; testicular ECA ACE ACE1, CD143, DCP, DCP1 angiotensin I converting enzyme angiotensin-converting enzyme|CD143 antigen|angiotensin I converting enzyme (peptidyl-dipeptidase A) 1|carboxycathepsin|dipeptidyl carboxypeptidase 1|dipeptidyl carboxypeptidase I|kininase II|peptidase P

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACE, check out the ACE Infographic

ACE infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00251 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Telmisartan protects chronic intermittent hypoxic mice via modulating cardiac renin-angiotensin system activity

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3 Customer Q&As for Anti-ACE1 Monoclonal Antibody

Question

Our team were well pleased with the WB result of your anti-ACE1 Monoclonal antibody. However we have seen positive staining in umbilical vein endothelial cell soluble form: using this antibody. Is that expected? Could you tell me where is ACE supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

Based on literature, umbilical vein endothelial cell does express ACE. Generally ACE expresses in angiotensin-converting enzyme, soluble form:, cell membrane. Regarding which tissues have ACE expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 2558109
Plasma, Pubmed ID: 16335952
Testis, Pubmed ID: 14702039
Umbilical vein endothelial cell, Pubmed ID: 9642152

Boster Scientific Support

Answered: 2019-12-05

Question

We are currently using anti-ACE1 Monoclonal antibody M00251 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-17

Answer

The anti-ACE1 Monoclonal antibody (M00251) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-17

Question

We have been able to see staining in mouse plasma. Any tips? Is anti-ACE1 Monoclonal antibody supposed to stain plasma positively?

V. Jackson

Verified customer

Asked: 2014-09-22

Answer

Based on literature plasma does express ACE. Based on Uniprot.org, ACE is expressed in testis, brain, lung, umbilical vein endothelial cell, plasma, liver, among other tissues. Regarding which tissues have ACE expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 2558109
Plasma, Pubmed ID: 16335952
Testis, Pubmed ID: 14702039
Umbilical vein endothelial cell, Pubmed ID: 9642152

Boster Scientific Support

Answered: 2014-09-22

Size

Total: $370

SKU:M00251

Backordered.

Lead time for this item is typically 3-4 weeks

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M00251
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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