|Pack Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum and plasma(heparin).|
Data & Images
|Product Name||Mouse ACE/Cd143 PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse ACE. 96wells/kit, with removable strips.|
|Cite This Product||Mouse ACE/Cd143 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0558)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: L35-Q1264|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, serum and plasma(heparin).
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from rat|
|Detection Antibody||polyclonal antibody from goat|
|Storage||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|EK0558-CAP||96-well plate precoated with anti-Mouse Ace antibody||1|
|EK0558-ST||lyophilized recombinant Mouse Ace standard||10ng/tubex2|
|EK0558-DA||biotinylated anti-Mouse Ace antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
Washing buffer Preparation: Disolve AR0030-E to 1000ml distilled water and adjust pH to 7.2~7.6. Finally, adjust the total volume to 1L.
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Angiotensin-converting enzyme|
|Molecular Weight||150918 MW|
|Protein Function||Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm. .|
|Tissue Specificity||Testis-specific isoform is expressed in spermatocytes, adult testis.|
|Subcellular Localization||Angiotensin-converting enzyme, soluble form: Secreted .|
|Alternative Names||Angiotensin-converting enzyme;ACE;3.2.1.-;126.96.36.199;Dipeptidyl carboxypeptidase I;Kininase II;CD143;Angiotensin-converting enzyme, soluble form;Ace;Dcp1;|
|Research Areas|||cardiovascular|blood|serum proteins| stem cells|hematopoietic progenitors|surface molecules| signal transduction|metabolism|vitamins / minerals| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|aces| cardiovascular|vasculature|vasoconstriction| cancer|cancer metabolism|response to hypoxia| kits/ lysates/ other|elisa kits|cardiovascular elisa kits| metabolism|pathways and processes|cofactors, vitamins / minerals|metabolism processes||
Background for Angiotensin-converting enzyme
Mouse ACE/Cd143 PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Mouse ACE/Cd143 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 20-25min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
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