Overview
| Product Name |
Human ACE/Cd143 ELISA Kit PicoKine™
|
| Catalog# |
EK0557 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Human ACE/Cd143 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACE in cell culture supernatants, serum, plasma (heparin) and saliva.. Sensitivity: 5pg/ml. |
| Cite This Product |
Human ACE/Cd143 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0557)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum, plasma (heparin) and saliva. |
| Sensitivity |
<5 pg/ml |
| Assay Range |
0.78 ng/ml - 50 ng/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: L30-L1261 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For ACE (Source: Uniprot.org, NCBI)
| Uniprot Id | B4DKH4 |
|---|
| Gene Name | ACE |
|---|
| Molecular Weight | 53750 MW |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ACE, check out the ACE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for ACE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the ACE infographic
Kit Components And Assay QC Data Details Of Human ACE/Cd143 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0557-CAP |
Anti-Human ACE Pre-coated 96-well strip microplate | 1 |
| EK0557-ST | Human ACE Standard | 2 vials, 50 ng/tube |
| EK0557-DA | Human ACE Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0557 Human ACE/Cd143 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.039 | 0.183 | 0.299 | 0.512 | 0.916 | 1.371 | 1.923 | 2.265 |
We measured random samples of Human ACE/Cd143 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1278 | 6839 | 24157 | 1358 | 6350 | 25528 |
| Standard deviation | 62.62 | 465.05 | 1087.06 | 86.57 | 482.6 | 1225.34 |
| CV (%) | 4.9% | 6.8% | 4.5% | 6.5% | 7.6% | 4.8% |
We measured the performance consistency of Human ACE/Cd143 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 1278 | 1310 | 1292 | 1425 | 1326 | 58.13 | 4.3% |
| Sample 2 | 6839 | 6994 | 7039 | 6669 | 6885 | 145.23 | 2.1% |
| Sample 3 | 24157 | 24884 | 22207 | 21600 | 23212 | 1350.66 | 5.8% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 4
| Protective Role of Antioxidant Huskless Barley Extracts on TNF-?-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells |
PubMed ID 29861828 |
| Role of Renin?Angiotensin-converting Enzyme Level and ACE Gene Polymorphism in Patients with Nonalcoholic Fatty Liver Disease |
PubMed ID 29201746 |
| Angiotensin(1-7) and ACE2, ?The Hot Spots? of Renin-Angiotensin System, Detected in the Human Aqueous Humor |
PubMed ID 25926900 |
| Autonomic Cardiovascular Responses in Acclimatized Lowlanders on Prolonged Stay at High Altitude: A Longitudinal Follow Up Study |
PubMed ID 24404157 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0557 Human ACE/Cd143 ELISA Kit PicoKine™
8 Related Questions
Question
Can the Human ACE PicoKine™ ELISA Kit (EK0557) detect ACE2 as well as ACE1?
Verified Customer
Asked: 2017-04-26
Answer
The immunogen of the antibodies in the Human ACE PicoKine™ ELISA Kit is the recombinant human ACE protein(L30-L1261) and was designed to detect only ACE1.
Boster Scientific Support
Answered: 2017-04-26
Question
Q: if the enzyme conjugated CD143 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD143 antigen?
T. Bhatt
Asked: 2020-05-03
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-05-03
Question
Q: can I use EDTA plasma as samples in Human CD143 Picokine® ELISA Kit (Catalog # EK0557)?
Verified Customer
Asked: 2020-03-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD143 causing degradation of its protein structure. CD143 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CD143 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-03-16
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD143 in Human saliva? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Asked: 2019-12-07
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD143 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-12-07
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CD143 using the EK0557 Human CD143 Picokine® ELISA Kit?
H. Anderson
Asked: 2018-05-01
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-05-01
Question
Q: What is the optimal O.D. value for CD143 ELISA kit? I used your CD143 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD143 even though the O.D. values are not very high?
Verified Customer
Asked: 2016-07-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD143 ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of CD143 that can be declared/interpreted as positive by the above standard is 5pg/ml.
Boster Scientific Support
Answered: 2016-07-15
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2014-02-24
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-02-24
Question
Is EK0557 suitable with humane bronchoalveolare Lavage (BAL) samples?
Answer
Unfortunately, our lab hasn't worked with humane bronchoalveolare Lavage (BAL) samples using the Human ACE/Cd143 ELISA Kit PicoKine™ (EK0557). We are uncertain if the kit is able to work for BAL samples. Please run a pilot test if you want to try. In theory, if the expression level of the target protein in the sample falls in the assay range of the kits, the kits are able to detect the proteins in the samples.
Boster Scientific Support
Answered: 2021-02-16
