Product Info Summary
| SKU: | EK0557 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin) and saliva. |
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Product info
Product Name
Human ACE/Cd143 ELISA Kit PicoKine®
SKU/Catalog Number
EK0557
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ACE/Cd143 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACE in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ACE/Cd143 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0557)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L30-L1261
Sensitivity
<5 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0557 is reactive to ACE in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) and saliva.
Application Guarantee
EK0557 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ACE/CD143
Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0557-CAP | Anti-Human ACE Pre-coated 96-well strip microplate | 1 |
| EK0557-ST | Human ACE Standard | 2 vials, 50 ng/tube |
| EK0557-DA | Human ACE Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.039 | 0.099 | 0.183 | 0.299 | 0.512 | 0.916 | 1.371 | 1.923 |
Data Example Images
Click image to see more details
Human ACE PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ACE/Cd143 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1278 | 6839 | 24157 | 1358 | 6350 | 25528 |
| Standard deviation | 62.62 | 465.05 | 1087.06 | 86.57 | 482.6 | 1225.34 |
| CV (%) | 4.9% | 6.8% | 4.5% | 6.5% | 7.6% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ACE/CD143 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1278 | 1310 | 1292 | 1425 | 1326 | 58.13 | 4.3% |
| Sample 2 | 6839 | 6994 | 7039 | 6669 | 6885 | 145.23 | 2.1% |
| Sample 3 | 24157 | 24884 | 22207 | 21600 | 23212 | 1350.66 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For ACE (Source: Uniprot.Org, NCBI)
Uniprot ID
P12821
Gene ID
1636
Gene Name
ACE
Full Name
Angiotensin-converting enzyme
Weight
149.715kDa
Superfamily
peptidase M2 family
Alternative Names
ACE; ACE1angiotensin converting enzyme, somatic isoform; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; carboxycathepsin; CD143 antigen; CD143; DCP; DCP1; DCP1angiotensin-converting enzyme; dipeptidyl carboxypeptidase 1; Dipeptidyl carboxypeptidase I; EC 3.2.1.-; EC 3.4.15.1; Kininase II; MGC26566; MVCD3; peptidase P; testicular ECA ACE ACE1, CD143, DCP, DCP1 angiotensin I converting enzyme angiotensin-converting enzyme|CD143 antigen|angiotensin I converting enzyme (peptidyl-dipeptidase A) 1|carboxycathepsin|dipeptidyl carboxypeptidase 1|dipeptidyl carboxypeptidase I|kininase II|peptidase P
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ACE, check out the ACE Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ACE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human ACE/Cd143 ELISA Kit PicoKine® (EK0557)
EK0557 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Autonomic Cardiovascular Responses in Acclimatized Lowlanders on Prolonged Stay at High Altitude: A Longitudinal Follow Up Study
Malvidin and its Glycosides from Vaccinium ashei Improve Endothelial Function by Anti-inflammatory and Angiotensin I-Converting Enzyme Inhibitory Effects:
Protective Role of Antioxidant Huskless Barley Extracts on TNF-α-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells
Differential responses of autonomic function in sea level residents, acclimatized lowlanders at >3500 m and Himalayan high altitude natives at >3500 m: A cross-sectional study
Combination of serum tumor markers dickkopf-1, DCP and AFP for the diagnosis of primary hepatocellular carcinoma
Angiotensin‐converting enzyme insertion/deletion gene polymorphism in Egyptian children with CAP: A case‐control study
Protective Role of Antioxidant Huskless Barley Extracts on TNF-?-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells
Role of Renin?Angiotensin-converting Enzyme Level and ACE Gene Polymorphism in Patients with Nonalcoholic Fatty Liver Disease
Angiotensin(1-7) and ACE2, ?The Hot Spots? of Renin-Angiotensin System, Detected in the Human Aqueous Humor
Autonomic Cardiovascular Responses in Acclimatized Lowlanders on Prolonged Stay at High Altitude: A Longitudinal Follow Up Study
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
8 Customer Q&As for Human ACE/Cd143 ELISA Kit PicoKine®
Question
Is EK0557 suitable with humane bronchoalveolare Lavage (BAL) samples?
Verified customer
Asked: 2021-02-12
Answer
Unfortunately, our lab hasn't worked with humane bronchoalveolare Lavage (BAL) samples using the Human ACE/Cd143 ELISA Kit PicoKine™ (EK0557). We are uncertain if the kit is able to work for BAL samples. Please run a pilot test if you want to try. In theory, if the expression level of the target protein in the sample falls in the assay range of the kits, the kits are able to detect the proteins in the samples.
Boster Scientific Support
Answered: 2021-02-16
Question
Q: if the enzyme conjugated CD143 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD143 antigen?
T. Bhatt
Verified customer
Asked: 2020-05-03
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-05-03
Question
Q: can I use EDTA plasma as samples in Human CD143 Picokine® ELISA Kit (Catalog # EK0557)?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD143 causing degradation of its protein structure. CD143 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CD143 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-03-16
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD143 in Human saliva? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-12-07
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD143 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-12-07
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CD143 using the EK0557 Human CD143 Picokine® ELISA Kit?
H. Anderson
Verified customer
Asked: 2018-05-01
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-05-01
Question
Can the Human ACE PicoKine™ ELISA Kit (EK0557) detect ACE2 as well as ACE1?
Verified Customer
Verified customer
Asked: 2017-04-26
Answer
The immunogen of the antibodies in the Human ACE PicoKine™ ELISA Kit is the recombinant human ACE protein(L30-L1261) and was designed to detect only ACE1.
Boster Scientific Support
Answered: 2017-04-26
Question
Q: What is the optimal O.D. value for CD143 ELISA kit? I used your CD143 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD143 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-07-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD143 ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of CD143 that can be declared/interpreted as positive by the above standard is 5pg/ml.
Boster Scientific Support
Answered: 2016-07-15
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2014-02-24
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-02-24
