Product Info Summary
SKU: | A08748 |
---|---|
Size: | 100μl |
Reactive Species: | Bovine, Human, Mouse, Pig, Rat |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-ACSM3 Monoclonal Antibody
SKU/Catalog Number
A08748
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ACSM3 Monoclonal Antibody catalog # A08748. Tested in WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACSM3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08748)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Immunogen
Purified recombinant human ACSM3 (C-terminal) protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08748 is reactive to ACSM3 in Bovine, Human, Mouse, Pig, Rat
Applications
A08748 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
66.153kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis using ACSM3 Monoclonal antibody against K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ACSM3 (Source: Uniprot.org, NCBI)
Gene Name
ACSM3
Full Name
Acyl-coenzyme A synthetase ACSM3, mitochondrial
Weight
66.153kDa
Superfamily
ATP-dependent AMP-binding enzyme family
Alternative Names
acyl-CoA synthetase medium-chain family member 3Protein SA homolog; acyl-coenzyme A synthetase ACSM3, mitochondrial; Butyrate--CoA ligase 3; Butyryl-coenzyme A synthetase 3; EC 6.2.1; Middle-chain acyl-CoA synthetase 3; SA (rat hypertension-associated) homolog; SA hypertension-associated homolog (rat); SA hypertension-associated homolog; SA; SAH; SAHEC 6.2.1.2 ACSM3 SA, SAH acyl-CoA synthetase medium chain family member 3 acyl-coenzyme A synthetase ACSM3, mitochondrial|SA (rat hypertension-associated) homolog|SA hypertension-associated homolog|butyrate--CoA ligase 3|butyryl-coenzyme A synthetase 3|middle-chain acyl-CoA synthetase 3|propionate--CoA ligase|protein SA homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACSM3, check out the ACSM3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACSM3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACSM3 Monoclonal Antibody (A08748)
Hello CJ!
No publications found for A08748
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-ACSM3 Monoclonal Antibody
Question
My question regarding product A08748, anti-ACSM3 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08748 anti-ACSM3 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-20
Question
We are currently using anti-ACSM3 Monoclonal antibody A08748 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says bovine, human, mouse, pig, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The anti-ACSM3 Monoclonal antibody (A08748) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-15
Question
Does anti-ACSM3 Monoclonal antibody A08748 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
According to the expression profile of kidney, ACSM3 is highly expressed in kidney. So, it is likely that anti-ACSM3 Monoclonal antibody A08748 will work for WB with kidney.
Boster Scientific Support
Answered: 2019-01-21
Question
I would like to test anti-ACSM3 Monoclonal antibody A08748 on human kidney for research purposes, then I may be interested in using anti-ACSM3 Monoclonal antibody A08748 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Z. Jones
Verified customer
Asked: 2018-07-12
Answer
The products we sell, including anti-ACSM3 Monoclonal antibody A08748, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-12
Question
I was wanting to use your anti-ACSM3 Monoclonal antibody for WB for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
As indicated on the product datasheet, A08748 anti-ACSM3 Monoclonal antibody has been validated for WB on bovine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-13
Question
Please see the WB image, lot number and protocol we used for kidney using anti-ACSM3 Monoclonal antibody A08748. Please let me know if you require anything else.
Z. Wu
Verified customer
Asked: 2014-11-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-11