Anti-ACSM3 Monoclonal Antibody

ACSM3 antibody

Boster Bio Anti-ACSM3 Monoclonal Antibody catalog # A08748. Tested in WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.

Product Info Summary

SKU: A08748
Size: 100μl
Reactive Species: Bovine, Human, Mouse, Pig, Rat
Host: Mouse
Application: WB

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Product Name

Anti-ACSM3 Monoclonal Antibody

View all ACSM3 Antibodies

SKU/Catalog Number

A08748

Size

100μl

Form

Liquid

Description

Boster Bio Anti-ACSM3 Monoclonal Antibody catalog # A08748. Tested in WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ACSM3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08748)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Clonality

Monoclonal

Clone Number

6H2

Isotype

IgG

Immunogen

Purified recombinant human ACSM3 (C-terminal) protein fragments expressed in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08748 is reactive to ACSM3 in Bovine, Human, Mouse, Pig, Rat

Applications

A08748 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

66.153kDa

Background of ACSM3

APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For ACSM3 (Source: Uniprot.org, NCBI)

Gene Name

ACSM3

Full Name

Acyl-coenzyme A synthetase ACSM3, mitochondrial

Weight

66.153kDa

Superfamily

ATP-dependent AMP-binding enzyme family

Alternative Names

acyl-CoA synthetase medium-chain family member 3Protein SA homolog; acyl-coenzyme A synthetase ACSM3, mitochondrial; Butyrate--CoA ligase 3; Butyryl-coenzyme A synthetase 3; EC 6.2.1; Middle-chain acyl-CoA synthetase 3; SA (rat hypertension-associated) homolog; SA hypertension-associated homolog (rat); SA hypertension-associated homolog; SA; SAH; SAHEC 6.2.1.2 ACSM3 SA, SAH acyl-CoA synthetase medium chain family member 3 acyl-coenzyme A synthetase ACSM3, mitochondrial|SA (rat hypertension-associated) homolog|SA hypertension-associated homolog|butyrate--CoA ligase 3|butyryl-coenzyme A synthetase 3|middle-chain acyl-CoA synthetase 3|propionate--CoA ligase|protein SA homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACSM3, check out the ACSM3 Infographic

ACSM3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACSM3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-ACSM3 Monoclonal Antibody

Question

My question regarding product A08748, anti-ACSM3 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08748 anti-ACSM3 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-20

Question

We are currently using anti-ACSM3 Monoclonal antibody A08748 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says bovine, human, mouse, pig, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The anti-ACSM3 Monoclonal antibody (A08748) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-15

Question

Does anti-ACSM3 Monoclonal antibody A08748 work for WB with kidney?

Verified Customer

Verified customer

Asked: 2019-01-21

Answer

According to the expression profile of kidney, ACSM3 is highly expressed in kidney. So, it is likely that anti-ACSM3 Monoclonal antibody A08748 will work for WB with kidney.

Boster Scientific Support

Answered: 2019-01-21

Question

I would like to test anti-ACSM3 Monoclonal antibody A08748 on human kidney for research purposes, then I may be interested in using anti-ACSM3 Monoclonal antibody A08748 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Z. Jones

Verified customer

Asked: 2018-07-12

Answer

The products we sell, including anti-ACSM3 Monoclonal antibody A08748, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-07-12

Question

I was wanting to use your anti-ACSM3 Monoclonal antibody for WB for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?

Verified Customer

Verified customer

Asked: 2017-11-13

Answer

As indicated on the product datasheet, A08748 anti-ACSM3 Monoclonal antibody has been validated for WB on bovine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-13

Question

Please see the WB image, lot number and protocol we used for kidney using anti-ACSM3 Monoclonal antibody A08748. Please let me know if you require anything else.

Z. Wu

Verified customer

Asked: 2014-11-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-11-11

Order DetailsPrice
A08748

100uL

$399

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A08748
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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