Overview
| Product Name |
Anti-Adiponectin/ADIPOQ Antibody Picoband™
See more information about Adiponectin/Acrp30, and browse related primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
PB9001 |
| Pack Size |
100μg/vial |
| Storage & Handling |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Description |
Polyclonal antibody for Adiponectin/ADIPOQ detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Adiponectin/ADIPOQ information: Molecular Weight: 26414 MW; Subcellular Localization: Secreted; Tissue Specificity: Synthesized exclusively by adipocytes and secreted into plasma. |
| Cite This Product |
Anti-Adiponectin/ADIPOQ Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9001)
|
| Antibodies Validation |
Antibodies Validation Information
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Anti-Adiponectin/ADIPOQ Antibody Picoband™ may replace the following items: sc 134814|sc 134815|sc 134816|sc 136131|sc 136794|sc 161992|sc 17044 R|sc 26496|sc 26497|sc 28019|sc 28020|sc 28021|sc 292399|sc 292399 X|sc 4862|sc 4863|sc 50108|sc 50109|sc 50110|sc 50111|sc 50113|sc 514273|sc 99578|sc 99579. |
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human |
| Applications |
IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
E.coli-derived human adiponectin recombinant protein (Position: E19-N244). Human adiponectin shares 85% amino acid (aa) sequence identity with mouse adiponectin. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For ADIPOQ (Source: Uniprot.org, NCBI)
| Uniprot Id | Q15848 |
|---|
| NCBI Gene Id | 9370 |
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| Species Of This Entry | Human |
|---|
| Gene Name | ADIPOQ |
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| Protein Name | Adiponectin |
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| Alternative Names | Adiponectin/Acrp30|ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein |
|---|
| Molecular Weight | 26414 |
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| Post Tranlational Modifications | Phosphorylation; Oxidation; Glycosylation; Acylation; Hydroxylation; Cleavage; Carboxylation; Ribosylation; Reduction; Ubiquitination; Nitration; Deacetylation; Acetylation; Methylation; Deglycosylation |
|---|
| Subcellular Localization | Secreted. |
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| Gene Location | 3q27.3, on Chromasome 3, gene sequence: NC_000003.12 (186842710..186858463) |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ADIPOQ (Source: Uniprot.org, NCBI)
| Protein Function | Significant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, improving glucose use and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in a variety of tissues such as liver and macrophages, and by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with different binding affinities, depending on the sort of complex, LMW, MMW or HMW. |
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| Tissue Specificity | Synthesized exclusively by adipocytes and secreted into plasma. |
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| Interacting Proteins | BNIP3L; CCDC155; SGTA |
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| Research Areas | Lipid and Metabolism |
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| Related Diseases | Obesity; Insulin Resistance; Diabetes Mellitus; Diabetes Mellitus, Non-insulin-dependent; Insulin Sensitivity; Inflammation; Cardiovascular Diseases; Metabolic Syndrome X; Atherosclerosis; Malignant Neoplasms; Coronary Artery Disease; Hypertensive Disease; Fatty Liver; Impaired Glucose Tolerance; Kidney Diseases; Liver Diseases; Neoplasms; Heart Diseases; Dyslipidemias |
|---|
| Related Pathways | Secretion; Pathogenesis; Glucose Homeostasis; Energy Homeostasis; Cell Proliferation; Insulin Secretion; Adiponectin Secretion; Inflammatory Response; Glomerular Filtration; Excretion; Cell Adhesion; Transport; Fatty Acid Oxidation; Immune Response; Aging; Bone Resorption; Cell Growth; Rna Interference; Localization; Angiogenesis |
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| Background | ADIPOQ (Adipocyte-, C1q-, and Collagen Domain-Containing),also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al. (2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al. (1999) showed that APM1 is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, they found links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Adiponectin is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones. |
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| Scientific References | PMID: 8619847 by Maeda K., et al. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1).
PMID: 10095105 by Saito K., et al. Organization of the gene for gelatin-binding protein (GBP28). |
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Our Boster Quality Guarantee for Anti-Adiponectin/ADIPOQ Antibody Picoband™ covers its use in the following applications.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti- Adiponectin antibody, PB9001, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti- Adiponectin antibody, PB9001, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti- Adiponectin antibody, PB9001, IHC(P)
IHC(P): Human Placenta Tissue
Anti- Adiponectin antibody, PB9001,Western blotting
All lanes: Anti Adiponectin (PB9001) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 30KD
Observed bind size: 30KD
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
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Total number of citations: 3
| Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev... |
PubMed ID 26077651 |
| Liu F, Mu J, Yuan Z, Wu G, Liu E, Zheng S, Lian Q, Ren K, Xu H. Nutrition. 2012 Apr;28(4):422-5. Doi: 10.1016/J.Nut.2011.08.018. Epub 2011 Dec 20. High Salt Intake Fails To Enhance Plasma Adiponectin In Normotensive Salt-Sensitive Subjects. |
PubMed ID 22189196 |
| Gao Y, Li F, Zhang Y, Dai L, Jiang H, Liu H, Zhang S, Chen C, Zhang J. Cell Physiol Biochem. 2013;31(2-3):452-61. Doi: 10.1159/000343381. Epub 2013 Mar 15. Silencing Of Adipoq Efficiently Suppresses Preadipocyte Differentiation In Porcine. |
PubMed ID 23548631 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to PB9001 Anti-Adiponectin/ADIPOQ Antibody Picoband™
16 Related Questions
Question
We ordered your anti-Adiponectin/ADIPOQ antibody for IHC on adipose tissue in a previous project. I am using human, and We are going to use the antibody for WB next. you antibody examining adipose tissue as well as adipose tissue of abdominal region in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Asked: 2020-04-03
Answer
I viewed the website and datasheets of our anti-Adiponectin/ADIPOQ antibody and it seems that PB9001 has been validated on human in both IHC and WB. Thus PB9001 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-03
Question
Please see the WB image, lot number and protocol we used for adipose tissue of abdominal region using anti-Adiponectin/ADIPOQ antibody PB9001. Please let me know if you require anything else.
Verified Customer
Asked: 2020-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-03
Question
I was wanting to use to test anti-Adiponectin/ADIPOQ antibody PB9001 on human adipose tissue of abdominal region for research purposes, then I may be interested in using anti-Adiponectin/ADIPOQ antibody PB9001 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Asked: 2019-12-30
Answer
The products we sell, including anti-Adiponectin/ADIPOQ antibody PB9001, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-30
Question
Would PB9001 anti-Adiponectin/ADIPOQ antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Asked: 2019-08-29
Answer
You can see on the product datasheet, PB9001 anti-Adiponectin/ADIPOQ antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-29
Question
My team were well pleased with the WB result of your anti-Adiponectin/ADIPOQ antibody. However we have observed positive staining in adipose tissue of abdominal region secreted. using this antibody. Is that expected? Could you tell me where is ADIPOQ supposed to be expressed?
Verified Customer
Asked: 2019-07-26
Answer
Based on literature, adipose tissue of abdominal region does express ADIPOQ. Generally ADIPOQ expresses in secreted. Regarding which tissues have ADIPOQ expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8619847
Boster Scientific Support
Answered: 2019-07-26
Question
We are currently using anti-Adiponectin/ADIPOQ antibody PB9001 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Asked: 2019-07-04
Answer
The anti-Adiponectin/ADIPOQ antibody (PB9001) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-04
Question
I have a question about product PB9001, anti-Adiponectin/ADIPOQ antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Asked: 2019-07-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9001 anti-Adiponectin/ADIPOQ antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-04
Question
I am looking for using your anti-Adiponectin/ADIPOQ antibody for negative regulation of macrophage derived foam cell differentiation studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Asked: 2019-07-02
Answer
Thanks for your inquiry. This PB9001 anti-Adiponectin/ADIPOQ antibody is tested on human placenta tissue, lung cancer tissue, tissue lysate, mammary cancer tissue, hela whole cell lysate, jurkat whole cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue of abdominal region using anti-Adiponectin/ADIPOQ antibody PB9001. Let me know if you need anything else.
Verified Customer
Asked: 2019-02-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-04
Question
Will anti-Adiponectin/ADIPOQ antibody PB9001 work for WB with adipose tissue of abdominal region?
M. Collins
Asked: 2018-08-28
Answer
According to the expression profile of adipose tissue of abdominal region, ADIPOQ is highly expressed in adipose tissue of abdominal region. So, it is likely that anti-Adiponectin/ADIPOQ antibody PB9001 will work for WB with adipose tissue of abdominal region.
Boster Scientific Support
Answered: 2018-08-28
Question
Is a blocking peptide available for product anti-Adiponectin/ADIPOQ antibody (PB9001)?
Verified Customer
Asked: 2018-07-10
Answer
We do provide the blocking peptide for product anti-Adiponectin/ADIPOQ antibody (PB9001). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-07-10
Question
I see that the anti-Adiponectin/ADIPOQ antibody PB9001 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Asked: 2018-05-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-31
Question
We have been able to see staining in human adipose tissue of abdominal region. What should we do? Is anti-Adiponectin/ADIPOQ antibody supposed to stain adipose tissue of abdominal region positively?
G. Lewis
Asked: 2017-12-21
Answer
According to literature adipose tissue of abdominal region does express ADIPOQ. According to Uniprot.org, ADIPOQ is expressed in adipose tissue of abdominal region, adipose tissue, among other tissues. Regarding which tissues have ADIPOQ expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8619847
Boster Scientific Support
Answered: 2017-12-21
Question
Is this PB9001 anti-Adiponectin/ADIPOQ antibody reactive to the isotypes of ADIPOQ?
Verified Customer
Asked: 2017-12-04
Answer
The immunogen of PB9001 anti-Adiponectin/ADIPOQ antibody is E.coli-derived human adiponectin recombinant protein (Position: E19-N244). Human adiponectin shares 85% amino acid (aa) sequence identity with mouse adiponectin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-04
Question
I was wanting to use your anti-Adiponectin/ADIPOQ antibody for WB for human adipose tissue of abdominal region on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adipose tissue of abdominal region identification?
B. Patel
Asked: 2017-01-10
Answer
You can see on the product datasheet, PB9001 anti-Adiponectin/ADIPOQ antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adipose tissue of abdominal region in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-10
Question
Do you have a BSA free version of anti-Adiponectin/ADIPOQ antibody PB9001 available?
R. Miller
Asked: 2013-10-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Adiponectin/ADIPOQ antibody PB9001 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-10-16
