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- Table of Contents
TXNIP antibody (A01409-1) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of THP-1 cells using anti-TXNIP antibody (A01409-1).
Overlay histogram showing THP-1 cells stained with A01409-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TXNIP Antibody (A01409-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A01409-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TXNIP Antibody (A01409-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TXNIP Antibody Picoband®
TXNIP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
TXNIP antibody was positively detected by WB in:
rat brain tissue, rat kidney tissue, rat PC-12 whole cell, mouse brain tissue, mouse kidney tissue, mouse NIH-3T3 whole cell, mouse RAW2647 whole cell, human K562 whole cell, human Hela whole cell, human HEK293 whole cell, human HL-60 whole cell, human A549 whole cell, human Caco-2 whole cell, human Raji whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TXNIP antibody was positively detected by IHC in:
human ovarian cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TXNIP antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TXNIP Antibody Picoband®
View all TXNIP Antibodies
View all TXNIP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TXNIP recombinant protein (Position: K5-Q391).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated Molecular Weight
43.7 kDa
Background of TXNIP
Thioredoxin-interacting protein is a protein that in humans is encoded by the TXNIP gene. It is mapped to 1q21.1. This gene encodes a thioredoxin-binding protein that is a member of the alpha arrestin protein family. Thioredoxin is a thiol-oxidoreductase that is a major regulator of cellular redox signaling which protects cells from oxidative stress. This protein inhibits the antioxidative function of thioredoxin resulting in the accumulation of reactive oxygen species and cellular stress. This protein also functions as a regulator of cellular metabolism and of endoplasmic reticulum (ER) stress. This protein may also function as a tumor suppressor. Alternate splicing results in multiple transcript variants.
Cite This Product
Anti-TXNIP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01409-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
98%
High Homology
Rat
97%
High Homology
Rabbit
97%
High Homology
Sheep
96%
High Homology
Mouse
96%
High Homology
Cow
95%
High Homology
Zebrafish
61%
Low homology
Drosophila
24%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TXNIP antibody
Immunohistochemistry (IHC) protocol for TXNIP antibody
Flow Cytometry (FC) protocol for TXNIP antibody
Publications
Application:
Species:
Upregulation of TXNIP contributes to granulosa cell dysfunction in polycystic ovary syndrome via activation of the NLRP3 inflammasome
Published: 2022-11-28
Journal: MOLECULAR AND CELLULAR ENDOCRINOLOGY
Application: IP,WB
Impact Factor: 4.37
Species: Mouse
PMID: 36450326
Identification and validation of a ferroptosis-related prognostic risk-scoring model and key genes in small cell lung cancer
Published: 2022-07-01
Journal: Translational Lung Cancer Research
Application: IHC
Impact Factor: 4.73
Species: Human
PMID: 35958330
Effects of Hypoxic Environment on Periodontal Tissue through the ROS/TXNIP/NLRP3 Inflammasome Pathway
Published: 2022-01-17
Journal: Biomed Research International
Application: WB,IF,ICC
Impact Factor: 3.25
Species: Rat
PMID: 35083332
Review
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1 Review For Anti-TXNIP Antibody Picoband®
Anti-TXNIP Antibody (A01409-1) delivered specific IHC staining with low background and confirmed weaker TXNIP expression in human colorectal carcinoma versus adjacent non-tumor tissues.
| SKU | A01409-1 |
|---|---|
| Application | Immunohistochemistry |
| Sample | Human colorectal cancer and adjacent non-tumor tissue samples |
| Sample Processing Description | ① Human colon cancer tissue ② Human adjacent non-tumor colon tissue |
| Other Reagents | Goat serum, Two-Step Histochemistry Kit |
| Primary Antibody | TXNIP Antibody Picoband® |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Secondary Antibody | Two-Step Histochemistry Kit |
| Secondary Incubation | Incubate at 37°C for 45 minutes |
| Detection | Imaging system: Leica DM2500 |
| Results Summary | TXNIP is widely recognized as an important tumor suppressor gene; it is downregulated in most cancers, and its expression level is significantly correlated with patient prognosis. Experimental results have also confirmed this. |
Xiang Li, China Pharmaceutical University
Verified customer
Submitted 2026-09-16
Product Promise
We promise Anti-TXNIP Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01409-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026