Rat Adiponectin ELISA Kit PicoKine®

Adiponectin/Acrp30 ELISA kit for Rat

Rat Adiponectin ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK1327
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Rat Adiponectin ELISA Kit PicoKine®

View all Adiponectin/Acrp30 ELISA kits

SKU/Catalog Number

EK1327

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat Adiponectin ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat Adiponectin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1327)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I19-N244

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1327 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1327 is reactive to ADIPOQ in Rat samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK1327 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Adiponectin/Acrp30

Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Kit Components

Catalog Number Description Quantity
EK1327-CAP Anti-Rat ADIPOQ Pre-coated 96-well strip microplate 1
EK1327-ST Rat ADIPOQ Standard 2 vials, 10 ng/tube
EK1327-DA Rat ADIPOQ Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0090.0590.1280.2270.4030.7061.2852.001

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Adiponectin/Acrp30, Dilution ratio of 1:2000, concentration in serum and plasma is 2.41-8.136 ug/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat Adiponectin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2921705426926516264075
Standard deviation16.93126.17170.7619.61133.33167.07
CV (%)5.8%7.4%4%7.4%8.2%4.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Adiponectin/Acrp30 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 129230729327729210.613.6%
Sample 217051526180717871706110.866.4%
Sample 342694351466746734490182.334%
*number of samples for each test n=16.

Gene/Protein Information For ADIPOQ (Source: Uniprot.Org, NCBI)

Gene Name

ADIPOQ

Full Name

Adiponectin

Weight

26.414kDa

Alternative Names

ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein ADIPOQ ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28 adiponectin, C1Q and collagen domain containing adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement-related 30 kDa protein|adipose most abundant gene transcript 1 protein|adipose specific collagen-like factor|gelatin-binding protein 28

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADIPOQ, check out the ADIPOQ Infographic

ADIPOQ infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ADIPOQ: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1327 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Combined Exposure to Fructose and Bisphenol A Exacerbates Abnormal Lipid Metabolism in Liver of Developmental Male Rats

Discovery of a Potent 9‐Deazaxanthine‐based Agent for the Treatment of Obesity‐Related Non‐alcoholic Fatty Liver Disease

Synthesis and Biological Evaluation of 5-Benzylidenepyrimidine-2,4,6(1H,3H,5H)-trione Derivatives for the Treatment of Obesity-Related Nonalcoholic Fatty Liver Disease

Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway

El Tabaa MM, Sokkar SS, Ramadan ES, Abd El Salam IZ, Zaid A. Neurochem Int. 2017 Mar 31. pii: S0197-0186(16)30518-6. doi: 10.1016/j.neuint.2017.03.019. Neuroprotective role of Ginkgo biloba against cognitive deficits associated with Bisphenol A ex...

Chen S, Li J, Zhang Z, Li W, Sun Y, Zhang Q, Feng X, Zhu W. Can J Physiol Pharmacol. 2012 Feb;90(2):237-42. Doi: 10.1139/Y11-123. Epub 2012 Feb 6. Effects Of Resveratrol On The Amelioration Of Insulin Resistance In Kkay Mice.

Hatami Zargaran Z, Salehi M, Heydari St, Babajafari S. Int Cardiovasc Res J. 2014 Apr;8(2):52-6. Epub 2014 Apr 1. The Effects Of 6 Isocaloric Meals On Body Weight, Lipid Profiles, Leptin, And Adiponectin In Overweight Subjects (Bmi ≫ 25).

Liu Xx, Liu Ky, Li P, Han S, Peng Xd, Shen L. Diabetes Metab. 2014 Nov;40(5):363-72. Doi: 10.1016/J.Diabet.2014.05.004. Epub 2014 Jun 27. Adiponectin Is Expressed In The Pancreas Of High-Fat-Diet-Fed Mice And Protects Pancreatic Endothelial Functi...

Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni...

Rasekhi H, Karandish M, Jalali Mt, Mohammadshahi M, Zarei M, Saki A, Shahbazian H. J Diabetes Metab Disord. 2015 Jan 14;14(1):1. Doi: 10.1186/S40200-014-0127-9. Ecollection 2015. Phylloquinone Supplementation Improves Glycemic Status Independent O...

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9 Customer Q&As for Rat Adiponectin ELISA Kit PicoKine®

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

B. Roberts

Verified customer

Asked: 2020-06-09

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-06-09

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

C. Collins

Verified customer

Asked: 2019-08-04

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-08-04

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Adiponectin using the EK1327 Rat Adiponectin Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-03-11

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-03-11

Question

Q: which procedure should I follow in order to thaw whole blood sample for Adiponectin ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-03-11

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Adiponectin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-03-11

Question

Q: if the enzyme conjugated Adiponectin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Adiponectin antigen?

H. Kulkarni

Verified customer

Asked: 2018-01-30

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-01-30

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2017-12-17

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-12-17

Question

Q: can I use citrate plasma as samples in Rat Adiponectin Picokine® ELISA Kit (Catalog # EK1327)?

S. Edwards

Verified customer

Asked: 2016-09-19

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Adiponectin causing degradation of its protein structure. Adiponectin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Adiponectin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-09-19

Question

Q: What is the optimal O.D. value for Adiponectin ELISA kit? I performed your Adiponectin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Adiponectin even though the O.D. values are not very high?

E. Yang

Verified customer

Asked: 2014-05-17

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Adiponectin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Adiponectin that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2014-05-17

Question

Q: Can Adiponectin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

P. Surve

Verified customer

Asked: 2014-01-04

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2014-01-04

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