Click image to see more details
Product Info Summary
| SKU: | M02014-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Actin ACTA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02014-2
BM4983 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Actin ACTA1 Rabbit Monoclonal Antibody catalog # M02014-2. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Actin ACTA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02014-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAOF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Actin
Reactive Species
M02014-2 is reactive to ACTA1 in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02014-2 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IP 1:50
Positive Control
WB: HeLa cell, NIH/3T3 cell, C6 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Actin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.
Specific Publications For Anti-Actin ACTA1 Rabbit Monoclonal Antibody (M02014-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Actin ACTA1 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Actin ACTA1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-Actin ACTA1 Rabbit Monoclonal Antibody
Question
We have seen staining in mouse skeletal muscle. Do you have any suggestions? Is anti-Actin Rabbit Monoclonal antibody supposed to stain skeletal muscle positively?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
According to literature skeletal muscle does express ACTA1. According to Uniprot.org, ACTA1 is expressed in biceps brachii, skeletal muscle, among other tissues. Regarding which tissues have ACTA1 expression, here are a few articles citing expression in various tissues:
Skeletal muscle, Pubmed ID: 6190133, 15489334
Boster Scientific Support
Answered: 2020-04-29
Question
We are currently using anti-Actin Rabbit Monoclonal antibody M02014-2 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
The anti-Actin Rabbit Monoclonal antibody (M02014-2) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-20
Question
I see that the anti-Actin Rabbit Monoclonal antibody M02014-2 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-Actin Rabbit Monoclonal antibody M02014-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-20
Question
See below the WB image, lot number and protocol we used for skeletal muscle using anti-Actin Rabbit Monoclonal antibody M02014-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-09
Question
Does anti-Actin Rabbit Monoclonal antibody M02014-2 work for IP with skeletal muscle?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
According to the expression profile of skeletal muscle, ACTA1 is highly expressed in skeletal muscle. So, it is likely that anti-Actin Rabbit Monoclonal antibody M02014-2 will work for IP with skeletal muscle.
Boster Scientific Support
Answered: 2019-08-07
Question
I have a question about product M02014-2, anti-Actin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02014-2 anti-Actin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-28
Question
My lab would like using your anti-Actin Rabbit Monoclonal antibody for skeletal muscle thin filament assembly studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
E. Kulkarni
Verified customer
Asked: 2018-11-06
Answer
Thank you for your inquiry. This M02014-2 anti-Actin Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-06
Question
Is this M02014-2 anti-Actin Rabbit Monoclonal antibody reactive to the isotypes of ACTA1?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
The immunogen of M02014-2 anti-Actin Rabbit Monoclonal antibody is A synthesized peptide derived from human Actin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-02
Question
We are interested in to test anti-Actin Rabbit Monoclonal antibody M02014-2 on human skeletal muscle for research purposes, then I may be interested in using anti-Actin Rabbit Monoclonal antibody M02014-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Miller
Verified customer
Asked: 2018-01-16
Answer
The products we sell, including anti-Actin Rabbit Monoclonal antibody M02014-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-16
Question
We ordered your anti-Actin Rabbit Monoclonal antibody for IP on skeletal muscle a few months ago. I am using human, and We are going to use the antibody for WB next. I would like examining skeletal muscle as well as biceps brachii in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
I have checked the website and datasheets of our anti-Actin Rabbit Monoclonal antibody and it appears that M02014-2 has been validated on human in both IP and WB. Thus M02014-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-05-26
Question
Is a blocking peptide available for product anti-Actin Rabbit Monoclonal antibody (M02014-2)?
S. Miller
Verified customer
Asked: 2017-04-18
Answer
We do provide the blocking peptide for product anti-Actin Rabbit Monoclonal antibody (M02014-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-04-18
Question
Does M02014-2 anti-Actin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Thomas
Verified customer
Asked: 2016-03-03
Answer
You can see on the product datasheet, M02014-2 anti-Actin Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-03
Question
I was wanting to use your anti-Actin Rabbit Monoclonal antibody for IP for human skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skeletal muscle identification?
N. Krishna
Verified customer
Asked: 2016-01-06
Answer
As indicated on the product datasheet, M02014-2 anti-Actin Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-06
Question
My boss were content with the WB result of your anti-Actin Rabbit Monoclonal antibody. However we have observed positive staining in biceps brachii cytoskeleton. using this antibody. Is that expected? Could you tell me where is ACTA1 supposed to be expressed?
T. Jones
Verified customer
Asked: 2015-05-19
Answer
From literature, biceps brachii does express ACTA1. Generally ACTA1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have ACTA1 expression, here are a few articles citing expression in various tissues:
Skeletal muscle, Pubmed ID: 6190133, 15489334
Boster Scientific Support
Answered: 2015-05-19
Question
Is there a BSA free version of anti-Actin Rabbit Monoclonal antibody M02014-2 available?
B. Baker
Verified customer
Asked: 2013-08-15
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Actin Rabbit Monoclonal antibody M02014-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-08-15
