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- Table of Contents
ADAM10 antibody (M00566) performs well in IP, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of ADAM10 using anti-ADAM10 antibody (M00566) .
ADAM10 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-ADAM10 Antibody (M00566) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
ADAM10 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-ADAM10 Antibody (M00566) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-ADAM10 Rabbit Monoclonal Antibody
ADAM10 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IHC, WB |
Tested Applications
ADAM10 antibody was positively detected by WB in:
human A431 whole cell, human Hacat whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ADAM10 antibody was positively detected by IHC in:
human rectum adenocarcinoma tissue, human glioblastoma tissue, human ovarian serous adenocarcinoma tissue, human lung adenocarcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Mouse
Recommend
Rat
Recommend
Product Information
Product Name
Anti-ADAM10 Rabbit Monoclonal Antibody
View all ADAM10 Antibodies
View all ADAM10 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAIC-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ADAM10
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
84 kDa
Calculated Molecular Weight
84.1 kDa
Cite This Product
Anti-ADAM10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00566)
Protocols
Western Blot protocol for ADAM10 antibody
Immunohistochemistry (IHC) protocol for ADAM10 antibody
Publications
Application:
Gemcitabine inhibits immune escape of pancreatic cancer by down regulating the soluble ULBP2 protein
PMID: 27602753
Review
Have you used Anti-ADAM10 Rabbit Monoclonal Antibody?
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1 Review For Anti-ADAM10 Rabbit Monoclonal Antibody
The antibody has good specificity, with a clear target band at the correct position and minimal non-specific bands.
| SKU | M00566 |
|---|---|
| Application | Western Blot |
| Sample | Human A549 cells, HCC1833 cells, LU65 cells, PC-9 cells |
| Sample Processing Description | After normal cell culture, total proteins were extracted using RIPA lysis buffer with protease inhibitors. Protein concentration was determined by BCA assay, and then the four samples were adjusted to a uniform concentration of 3 mg/mL using loading buffer. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | c-Fos Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Goat Anti-Rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The purpose of the experiment is to examine the differences in ADAM10 protein expression among different types of human lung cancer cells, in order to select suitable cells for subsequent experiments. |
Xu Hu, School of Life Sciences, Fudan University
Verified customer
Submitted 2025-12-11
Product Promise
We promise Anti-ADAM10 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M00566
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026