Product Info Summary
SKU: | M00566 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, WB |
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Product info
Product Name
Anti-ADAM10 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00566
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ADAM10 Rabbit Monoclonal Antibody catalog # M00566. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADAM10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00566)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAIC-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ADAM10
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00566 is reactive to ADAM10 in Human, Mouse, Rat
Applications
M00566 is guaranteed for IP, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
84.142kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ADAM10 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ADAM10 (Source: Uniprot.org, NCBI)
Gene Name
ADAM10
Full Name
Disintegrin and metalloproteinase domain-containing protein 10
Weight
84.142kDa
Alternative Names
a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease ADAM10 AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz ADAM metallopeptidase domain 10 disintegrin and metalloproteinase domain-containing protein 10|a disintegrin and metalloprotease domain 10|kuzbanian protein homolog|mammalian disintegrin-metalloprotease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADAM10, check out the ADAM10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADAM10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADAM10 Rabbit Monoclonal Antibody (M00566)
Hello CJ!
M00566 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gemcitabine inhibits immune escape of pancreatic cancer by down regulating the soluble ULBP2 protein
Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-ADAM10 Rabbit Monoclonal Antibody
Question
I see that the anti-ADAM10 Rabbit Monoclonal antibody M00566 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-ADAM10 Rabbit Monoclonal antibody M00566. Let me know if you need anything else.
J. Gonzalez
Verified customer
Asked: 2020-03-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-13
Question
Can you help my question with product M00566, anti-ADAM10 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00566 anti-ADAM10 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-06
Question
We have observed staining in mouse platelet. Do you have any suggestions? Is anti-ADAM10 Rabbit Monoclonal antibody supposed to stain platelet positively?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
From literature platelet does express ADAM10. From Uniprot.org, ADAM10 is expressed in testis, placenta, platelet, liver, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699
Boster Scientific Support
Answered: 2020-01-17
Question
I was wanting to use your anti-ADAM10 Rabbit Monoclonal antibody for WB for rat testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat testis identification?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
As indicated on the product datasheet, M00566 anti-ADAM10 Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-09
Question
I am interested in to test anti-ADAM10 Rabbit Monoclonal antibody M00566 on rat testis for research purposes, then I may be interested in using anti-ADAM10 Rabbit Monoclonal antibody M00566 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The products we sell, including anti-ADAM10 Rabbit Monoclonal antibody M00566, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-03
Question
I would like using your anti-ADAM10 Rabbit Monoclonal antibody for degradation of the extracellular matrix studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
We appreciate your inquiry. This M00566 anti-ADAM10 Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-14
Question
Is a blocking peptide available for product anti-ADAM10 Rabbit Monoclonal antibody (M00566)?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
We do provide the blocking peptide for product anti-ADAM10 Rabbit Monoclonal antibody (M00566). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-14
Question
Do you have a BSA free version of anti-ADAM10 Rabbit Monoclonal antibody M00566 available?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ADAM10 Rabbit Monoclonal antibody M00566 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-26
Question
We are currently using anti-ADAM10 Rabbit Monoclonal antibody M00566 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
The anti-ADAM10 Rabbit Monoclonal antibody (M00566) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-20
Question
Please see the WB image, lot number and protocol we used for testis using anti-ADAM10 Rabbit Monoclonal antibody M00566. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-18
Question
Would M00566 anti-ADAM10 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-02
Answer
You can see on the product datasheet, M00566 anti-ADAM10 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-02
Question
My team were content with the WB result of your anti-ADAM10 Rabbit Monoclonal antibody. However we have seen positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is ADAM10 supposed to be expressed?
K. Evans
Verified customer
Asked: 2019-01-18
Answer
According to literature, placenta does express ADAM10. Generally ADAM10 expresses in cell membrane. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699
Boster Scientific Support
Answered: 2019-01-18
Question
We bought anti-ADAM10 Rabbit Monoclonal antibody for WB on cervix carcinoma a few years ago. I am using rat, and I plan to use the antibody for IP next. We are interested in examining cervix carcinoma as well as platelet in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
I looked at the website and datasheets of our anti-ADAM10 Rabbit Monoclonal antibody and it appears that M00566 has been tested on rat in both WB and IP. Thus M00566 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-03
Question
Is this M00566 anti-ADAM10 Rabbit Monoclonal antibody reactive to the isotypes of ADAM10?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
The immunogen of M00566 anti-ADAM10 Rabbit Monoclonal antibody is A synthesized peptide derived from human ADAM10. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-19
Question
Does anti-ADAM10 Rabbit Monoclonal antibody M00566 work for WB with testis?
E. Baker
Verified customer
Asked: 2016-09-29
Answer
According to the expression profile of testis, ADAM10 is highly expressed in testis. So, it is likely that anti-ADAM10 Rabbit Monoclonal antibody M00566 will work for WB with testis.
Boster Scientific Support
Answered: 2016-09-29