Anti-ADAM10 Rabbit Monoclonal Antibody

ADAM10 antibody

Boster Bio Anti-ADAM10 Rabbit Monoclonal Antibody catalog # M00566. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M00566
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, WB

Product Name

Anti-ADAM10 Rabbit Monoclonal Antibody

View all ADAM10 Antibodies

SKU/Catalog Number

M00566

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ADAM10 Rabbit Monoclonal Antibody catalog # M00566. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADAM10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00566)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAIC-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ADAM10

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00566 is reactive to ADAM10 in Human, Mouse, Rat

Applications

M00566 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

84.142kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ADAM10 (Source: Uniprot.org, NCBI)

Gene Name

ADAM10

Full Name

Disintegrin and metalloproteinase domain-containing protein 10

Weight

84.142kDa

Alternative Names

a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease ADAM10 AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz ADAM metallopeptidase domain 10 disintegrin and metalloproteinase domain-containing protein 10|a disintegrin and metalloprotease domain 10|kuzbanian protein homolog|mammalian disintegrin-metalloprotease

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADAM10, check out the ADAM10 Infographic

ADAM10 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADAM10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00566 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gemcitabine inhibits immune escape of pancreatic cancer by down regulating the soluble ULBP2 protein

Have you used Anti-ADAM10 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-ADAM10 Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-ADAM10 Rabbit Monoclonal Antibody

Question

I see that the anti-ADAM10 Rabbit Monoclonal antibody M00566 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-16

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-ADAM10 Rabbit Monoclonal antibody M00566. Let me know if you need anything else.

J. Gonzalez

Verified customer

Asked: 2020-03-13

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-13

Question

Can you help my question with product M00566, anti-ADAM10 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-06

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00566 anti-ADAM10 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-06

Question

We have observed staining in mouse platelet. Do you have any suggestions? Is anti-ADAM10 Rabbit Monoclonal antibody supposed to stain platelet positively?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

From literature platelet does express ADAM10. From Uniprot.org, ADAM10 is expressed in testis, placenta, platelet, liver, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699

Boster Scientific Support

Answered: 2020-01-17

Question

I was wanting to use your anti-ADAM10 Rabbit Monoclonal antibody for WB for rat testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat testis identification?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

As indicated on the product datasheet, M00566 anti-ADAM10 Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-09

Question

I am interested in to test anti-ADAM10 Rabbit Monoclonal antibody M00566 on rat testis for research purposes, then I may be interested in using anti-ADAM10 Rabbit Monoclonal antibody M00566 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

The products we sell, including anti-ADAM10 Rabbit Monoclonal antibody M00566, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-03

Question

I would like using your anti-ADAM10 Rabbit Monoclonal antibody for degradation of the extracellular matrix studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

We appreciate your inquiry. This M00566 anti-ADAM10 Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-14

Question

Is a blocking peptide available for product anti-ADAM10 Rabbit Monoclonal antibody (M00566)?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

We do provide the blocking peptide for product anti-ADAM10 Rabbit Monoclonal antibody (M00566). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-14

Question

Do you have a BSA free version of anti-ADAM10 Rabbit Monoclonal antibody M00566 available?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ADAM10 Rabbit Monoclonal antibody M00566 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-26

Question

We are currently using anti-ADAM10 Rabbit Monoclonal antibody M00566 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

The anti-ADAM10 Rabbit Monoclonal antibody (M00566) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-20

Question

Please see the WB image, lot number and protocol we used for testis using anti-ADAM10 Rabbit Monoclonal antibody M00566. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-18

Question

Would M00566 anti-ADAM10 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-04-02

Answer

You can see on the product datasheet, M00566 anti-ADAM10 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-02

Question

My team were content with the WB result of your anti-ADAM10 Rabbit Monoclonal antibody. However we have seen positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is ADAM10 supposed to be expressed?

K. Evans

Verified customer

Asked: 2019-01-18

Answer

According to literature, placenta does express ADAM10. Generally ADAM10 expresses in cell membrane. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699

Boster Scientific Support

Answered: 2019-01-18

Question

We bought anti-ADAM10 Rabbit Monoclonal antibody for WB on cervix carcinoma a few years ago. I am using rat, and I plan to use the antibody for IP next. We are interested in examining cervix carcinoma as well as platelet in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?

Verified Customer

Verified customer

Asked: 2018-05-03

Answer

I looked at the website and datasheets of our anti-ADAM10 Rabbit Monoclonal antibody and it appears that M00566 has been tested on rat in both WB and IP. Thus M00566 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-05-03

Question

Is this M00566 anti-ADAM10 Rabbit Monoclonal antibody reactive to the isotypes of ADAM10?

Verified Customer

Verified customer

Asked: 2018-01-19

Answer

The immunogen of M00566 anti-ADAM10 Rabbit Monoclonal antibody is A synthesized peptide derived from human ADAM10. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-19

Question

Does anti-ADAM10 Rabbit Monoclonal antibody M00566 work for WB with testis?

E. Baker

Verified customer

Asked: 2016-09-29

Answer

According to the expression profile of testis, ADAM10 is highly expressed in testis. So, it is likely that anti-ADAM10 Rabbit Monoclonal antibody M00566 will work for WB with testis.

Boster Scientific Support

Answered: 2016-09-29

Size

Total: $370

SKU:M00566

Backordered.

Lead time for this item is typically 3-4 weeks

Get A Quote
Eddy test
In stock
Order Product
M00566
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.