Product Info Summary
SKU: | A03622 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF |
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Product info
Product Name
Anti-Adrenergic Receptor alpha-2B ADRA2B Antibody
View all alpha-2B Adrenergic R/ADRA2B Antibodies
SKU/Catalog Number
A03622
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Adrenergic Receptor alpha-2B ADRA2B Antibody (Catalog # A03622). Tested in IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Adrenergic Receptor alpha-2B ADRA2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03622)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from internal of human Adrenergic Receptor alpha-2B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03622 is reactive to ADRA2B in Human
Applications
A03622 is guaranteed for IF Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
49.954kDa
Background of alpha-2B Adrenergic R/ADRA2B
Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is clonidine > norepinephrine > epinephrine = oxymetazoline > dopamine > p-tyramine = phenylephrine > serotonin > p-synephrine / p-octopamine. For antagonists, the rank order is yohimbine > chlorpromazine > phentolamine > mianserine > spiperone > prazosin > alprenolol > propanolol > pindolol.
Lomasney J.W., Proc. Natl. Acad. Sci. U.S.A. 87:5094-5098(1990).
Weinshank R.L., Mol. Pharmacol. 38:681-688(1990).
Small K.M., J. Biol. Chem. 276:4917-4922(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of HepG2 cells, using Adrenergic Receptor alpha-2B antibody A03622.
Protein Target Info & Infographic
Gene/Protein Information For ADRA2B (Source: Uniprot.org, NCBI)
Gene Name
ADRA2B
Full Name
Alpha-2B adrenergic receptor
Weight
49.954kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
a-2B AdrenergicR; ADRA2B adrenergic, alpha-2B-, receptor; ADRA2B; ADRA2L1; ADRA2L1adrenergic receptor alpha 2B; ADRA2RL1; ADRARL1; adrenergic, alpha-2B-, receptor; Alpha-2 adrenergic receptor subtype C2; alpha-2-adrenergic receptor-like 1; alpha-2B Adrenergic R; alpha-2B adrenergic receptor; alpha2B AdrenergicR; Alpha-2B adrenoceptor; Alpha-2B adrenoreceptor; alpha-2B-adrenergic receptor; ALPHA2BAR; alpha-2BAR; G-protein coupled receptor ADRA2B ADRA2L1, ADRA2RL1, ADRARL1, ALPHA2BAR, FAME2, alpha-2BAR adrenoceptor alpha 2B alpha-2B adrenergic receptor|ADRA2B adrenergic, alpha-2B-, receptor|G-protein coupled receptor|alpha-2 adrenergic receptor subtype C2|alpha-2-adrenergic receptor-like 1|alpha-2B adrenoreceptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADRA2B, check out the ADRA2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADRA2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Adrenergic Receptor alpha-2B ADRA2B Antibody (A03622)
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No publications found for A03622
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Adrenergic Receptor alpha-2B ADRA2B Antibody
Question
I was wanting to use your anti-Adrenergic Receptor alpha-2B antibody for IF for human apex of heart on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human apex of heart identification?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
You can see on the product datasheet, A03622 anti-Adrenergic Receptor alpha-2B antibody has been tested for IF on human tissues. We have an innovator award program that if you test this antibody and show it works in human apex of heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-11
Question
I see that the anti-Adrenergic Receptor alpha-2B antibody A03622 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-25
Question
We are currently using anti-Adrenergic Receptor alpha-2B antibody A03622 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
R. Evans
Verified customer
Asked: 2015-06-15
Answer
The anti-Adrenergic Receptor alpha-2B antibody (A03622) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-06-15
Question
you antibody to test anti-Adrenergic Receptor alpha-2B antibody A03622 on human apex of heart for research purposes, then I may be interested in using anti-Adrenergic Receptor alpha-2B antibody A03622 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Williams
Verified customer
Asked: 2013-07-18
Answer
The products we sell, including anti-Adrenergic Receptor alpha-2B antibody A03622, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-07-18