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- Table of Contents
FDX1 antibody (M05441) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IF analysis of FDX1 using anti-FDX1 antibody (M05441).
FDX1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-FDX1 Antibody (M05441) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
FDX1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-FDX1 Antibody (M05441) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-ADX Rabbit Monoclonal Antibody
FDX1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
FDX1 antibody was positively detected by WB in:
human A549 whole cell, human CACO-2 whole cell, human SiHa whole cell, human 293T whole cell, rat PC-12 whole cell, rat C6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FDX1 antibody was positively detected by ICC/IF in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Recommend
Product Information
Product Name
Anti-ADX Rabbit Monoclonal Antibody
View all FDX1 Antibodies
View all FDX1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
28F80
Isotype
IgG
Immunogen
A synthesized peptide derived from human ADX
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
13 kDa
Calculated Molecular Weight
19.4 kDa
Cite This Product
Anti-ADX Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05441)
Protocols
Western Blot protocol for FDX1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FDX1 antibody
Publications
Application:
Species:
UBE2S Promotes KIRC Progression by Regulating CDC20 Expression and Suppressing FDX1-Mediated Cuproptosis, While Promoting M2 Macrophage Polarization
Published: 2026-08-30
Journal: MOLECULAR CARCINOGENESIS
Application: WB,IP
Impact Factor: 3.50
Species: Human
PMID: 42669192
Shenxiaohuangqi decoction alleviates oxaliplatin-induced peripheral neuropathy by inhibiting cuproptosis
Published: 2025-11-14
Journal: Journal of Radiation Research and Applied Sciences
Application: IHC
Impact Factor: 2.50
Species: Rat
Hypoxia-induced autophagy attenuates ferredoxin 1-mediated cuproptosis in colorectal cancer cells
Published: 2025-04-28
Journal: HUMAN & EXPERIMENTAL TOXICOLOGY
Application: WB
Impact Factor: 3.20
Species: Human
PMID: 40294285
Copper metabolism-related risk score identifies hepatocellular carcinoma subtypes and SLC27A5 as a potential regulator of cuproptosis
Published: 2023-12-28
Journal: Aging-US
Application: WB
Impact Factor: 5.20
Species: Human
PMID: 38157255
Cuproptosis-related gene FDX1 as a prognostic biomarker for kidney renal clear cell carcinoma correlates with immune checkpoints and immune cell infiltration
Published: 2023-01-23
Journal: Frontiers in Genetics
Application: IHC
Impact Factor: 3.70
Species: Human
PMID: 36755576
Review
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Product Promise
We promise Anti-ADX Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M05441
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026